LOCUS       sequence-232119-       10377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hH1:mCherry (nuclear marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1170R
FEATURES             Location/Qualifiers
     source          1..10377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1061..1768
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1471..1490
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2161..2712
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(3602..3619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3602..3618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3611..3633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3821..3845
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5146..5775
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6204..7277
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7343..7537
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7773..7795)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7881..8021
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7936..7955
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8036..8053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8207..8795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8287..8306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8882..9676)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10101..10125
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10203..10377
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagcctc ccaagaagga aaccaaggct gagggcgctg cccccaagac accttcgcac
       61 cctacctacc aggtttgtct cgccattttt tcttcttcga cttcaagacg ctcgatatag
      121 ttcattggat gctaacatat tacaggctat gatcactgat gccatcatca acgtaattgc
      181 tcccgcttcc caaattccgg cgtgtcacgg gcgcgtcgga gacttgatgg ctgttctctc
      241 tttgatcacg caaccagaca tttggttatt ctagatcatg agaccacttt tgaacctatg
      301 gcatgacacc agattatgtt gctgactctt ctttttgcca ctcttcagct caaggaccgt
      361 aatggcagca ggtaatttcc cctttcattt tattctcgtt tgcaacctca gatgcatcac
      421 gtgctaactc gattcaccag ccgcccgcaa ctcaagaagt atgtcaaggc caacaacaag
      481 ctcggcgagg taacggacag catgttcgac tcgcttttca accgtgccct caaggccggt
      541 gtcgacaagg gagtgtttga gcaacccaag ggtaagttat gtgacagtcg atattttcat
      601 tgtgcaatct ctaataagaa ttacaggacc atctggaggt accaagctgg ccaagaaggc
      661 tccgcccaag aaggaggctg ctcctaagaa ggtggccaag aaggacgacg ccaaggccgc
      721 accaaagaag gccgcaccga agaaggcggc agctcccaag gagaaggaga aggacgccgc
      781 cgaaaagcct gctgccccca agaaggcggc tcccaagaag cccgctgccc ccaagaagga
      841 cgccgctccc aaggagaaga aggagcccaa ggagaagaag cccgccgcag acaaggcact
      901 cagcaagacc aagactggac gtgtttctaa gactgctgct ccagccaagc cagcagcagc
      961 caagaaggcc gcaccgaaga aggcaacggc cccgaagaag gccaccccca agaagactga
     1021 aaaggccgag gcacccgccg cagaattcga gctcggtacc gtgagcaagg gcgaggagga
     1081 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     1141 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1201 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1261 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1321 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1381 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1441 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     1501 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     1561 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     1621 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     1681 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     1741 cggcggcatg gacgagctgt acaagtaagg atccactaga gtcgaggata ttgaaggagc
     1801 actttttggg cttggctgga gctagtggag gtcaacaatg aatgcctatt ttggtttagt
     1861 cgtccaggcg gtgagcacaa aatttgtgtc gtttgacaag atggttcatt taggcaactg
     1921 gtcagatcag ccccacttgt agcagtagcg gcggcgctcg aagtgtgact cttattagca
     1981 gacaggaacg aggacattat tatcatctgc tgcttggtgc acgataactt ggtgcgtttg
     2041 tcaagcaagg taagtgaacg acccggtcat accttcttaa gttcgccctt cctcccttta
     2101 tttcagattc aatctgactt acctattcta cccaagcaac gcttcgatta ggaagtaacc
     2161 atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg
     2221 gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg
     2281 caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc
     2341 gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggtccctg gaaggcacgc
     2401 aacgcctacg actggacggc cgaatcgacc gtgtacgtct ccccccgcca ccagcggacg
     2461 ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag
     2521 agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc
     2581 ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac
     2641 gtgggtttct ggcagctgga cttcagcctg ccggttccgc cccgtccggt cctgcccgtc
     2701 accgagattt aggtcgacct gcagtaaagg gacgctgacg aacttttttg ttacttcata
     2761 tttcttcttc atttgtcttt tcactttggc gtttagagcg tttcttatgg gtaaattctt
     2821 ttgggaggtg cgacggaaag ctatctgggg ttcagtgtct gggattttta tttctgtctt
     2881 gtgtttctgt gttcaccgca gggctgtgat gctttgggcc cttttttttc catgtctctt
     2941 gtcatgacgc gtgcgggtgg cgggccttgg gcgtatgagt cgtgtgtctt gataccattt
     3001 ggcgcgcgcg cagggtcctc ttgagatttt ttttgggggc gattgtggga gggttttcaa
     3061 agctgggatg cgcgtataat tataccacaa atgaatgcat ggtgaatatt tcaaattctt
     3121 gtttcttttg ccaatcggga gcttgtcgat tagtcttgtc cttgatgcgc ctttttcgaa
     3181 ccgtttgaga tttgtttact tacttagatt ctatcccatg cccagacgcg tgctggaaat
     3241 ctcgctcgct tggtttggtc agggggtgtc acgtgggaat agagccacct tttatgtccg
     3301 tgcttactta gtactgctat cttctgtcca tgcgcccaac tgactgactg ttcaatccag
     3361 tcacgcacgc tcacctcagc aatcacgagt gctactgtgt gtggatggtg ccttggctaa
     3421 agtactgtac gtcacgggcg cttaggttca tccaaccctg ggcagacttg aattgattgg
     3481 gaatgggata tctgccttgc agacgacgga tggaaatgtc catagcggaa taaaacgaca
     3541 aaggaaaggg ttctacagta cggcaagggt ttccctttcc cgtcaaagaa agaagcttgg
     3601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3721 gcccttccca acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc
     3781 agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg
     3841 taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag
     3901 gtttatccgt tggtccattt gtatgtccat gccaaccaca gggttcccct cgggatcaaa
     3961 gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     4021 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     4081 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     4141 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     4201 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     4261 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     4321 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     4381 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     4441 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     4501 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     4561 ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     4621 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     4681 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     4741 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     4801 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     4861 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     4921 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
     4981 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     5041 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     5101 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     5161 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     5221 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     5281 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     5341 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     5401 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     5461 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     5521 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     5581 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     5641 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     5701 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     5761 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     5821 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     5881 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     5941 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     6001 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     6061 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     6121 acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga
     6181 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     6241 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     6301 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     6361 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     6421 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     6481 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     6541 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6601 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     6661 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     6721 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     6781 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     6841 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     6901 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6961 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     7021 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     7081 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     7141 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     7201 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     7261 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     7321 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     7381 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     7441 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     7501 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     7561 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     7621 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     7681 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     7741 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7801 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7861 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     7921 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7981 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     8041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     8881 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
     8941 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
     9001 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
     9061 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     9121 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     9181 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     9241 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     9301 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     9361 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     9421 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     9481 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
     9541 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     9601 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     9661 ttctcatttt agccatttat tatttccttc ctcttttcta tagtatttaa agatacccca
     9721 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     9781 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     9841 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     9901 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     9961 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
    10021 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
    10081 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
    10141 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
    10201 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
    10261 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
    10321 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaa
//
