LOCUS       sequence-232134-       10478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CenpC:eGFP (centromere marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1079
FEATURES             Location/Qualifiers
     source          1..10478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(568..570,571..1284)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(568..570,571..1281)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(610..631)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(871..890)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1218..1239
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1286..1302)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1286..1302)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1666..2691
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     primer_bind     complement(3703..3720)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3703..3719)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3712..3734)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3922..3946
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5247..5876
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6305..7378
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7444..7638
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7874..7896)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7982..8122
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8037..8056
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8137..8154)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8308..8896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8388..8407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8983..9777)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10202..10226
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    10304..10478
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt cgaggacgaa gaaccttcac cctgggaaca ggatcctggc gagctgatcg
       61 tgccagtcca gcagtggcag cctgagcacg aatttgaccc acccgggcct gatgacgagc
      121 tgcccgaggg cgaggacgta gtagcggtat caggaccagc gattaggact cgcgagatcc
      181 ttgggtcgag cttccggttc accaagacgc taagcttacc cttctttgga actggagtgg
      241 tggatctacc gccaggggct gagaaaaagg ccaagaactc acgcaagatg cacatgacct
      301 ttatagtgca cacggggcgg gtcactgtga cggtcaacaa cacggccttt tcgattggca
      361 agggtggcat gtggtttgtg cccagaggta tgtgtgcggt ccttttcatg tgttatgcgc
      421 ctcaaatact gatatatttc tacaggcaac tattacagca ttgcgaatga gggcgccaag
      481 tcgtccagga tattcttcgc gcaggcttgt gaatatagaa gacctgatga agatgagcag
      541 gacgaaatga ctgaaagcag cggtaccgtg agcaagggcg aggagctgtt caccggggtg
      601 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      661 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      721 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      781 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      841 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      901 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      961 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1021 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1081 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1141 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1201 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1261 ggcatggacg agctgtacaa gtaaggatcc actagttcta gagtcgagga tattgaagga
     1321 gcactttttg ggcttggctg gagctagtgg aggtcaacaa tgaatgccta ttttggttta
     1381 gtcgtccagg cggtgagcac aaaatttgtg tcgtttgaca agatggttca tttaggcaac
     1441 tggtcagatc agccccactt gtagcagtag cggcggcgct cgaagtgtga ctcttattag
     1501 cagacaggaa cgaggacatt attatcatct gctgcttggt gcacgataac ttggtgcgtt
     1561 tgtcaagcaa ggtaagtgga cgacccggtc ataccttctt aagttcgccc ttcctccctt
     1621 tatttcagat tcaatctgac ttacctattc tacccaagca tccaaatgaa aaagcctgaa
     1681 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
     1741 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     1801 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     1861 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga gtttagcgag
     1921 agcctgacct attgcatctc ccgccgttca cagggtgtca cgttgcaaga cctgcctgaa
     1981 accgaactgc ccgctgttct acaaccggtc gcggaggcta tggatgcgat cgctgcggcc
     2041 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     2101 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     2161 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     2221 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     2281 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     2341 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     2401 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccacgact ccgggcgtat
     2461 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2521 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2581 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2641 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaagaaata gagtagatgc
     2701 cgaccggaac cagtcgacct gcagtaattc atcgcggtgg gttggtctgg ttgacggata
     2761 aaaaagtatg ataccaatgt tgacgatctg ttttacgccc aattcttgtt tacctagctc
     2821 attgtctgta tacccatttg aaccctgcct aacacgtatt ctttctaatc tttgggtcgt
     2881 attgcttgag gatcctgtgc atgtgatgtg attgcgttgc tttgaattaa atacaacggg
     2941 ctcgacccag gtacgaccct ctttacctga cttttcacct gtccttctat ctaccttcca
     3001 tttttactta tattcgcgtc taccttctca gctaaaacca gttgaaaccc ccggaataat
     3061 attaatcaaa cctaagccga aacggcatta tggcattgta ttcaggctgt tcccgccgcg
     3121 atttagagac ttagaaggac tgctagactc ccagctatgt gaatgacttc agttaaaacc
     3181 agcagaaact cccgaaacga tattgatcaa accccaagcc aaaatcccat tccatgcgtt
     3241 atttccagcc cgtttccacc gcgatttcat caaagcagcc gagaaatact cttgccatcg
     3301 ataacagcga tcagattcta gctcttcgtc agggccgaat atccaccctg gcagcctcgc
     3361 tgccaaccat gccgtctcgg agctgttgct gcaactgcgc cgtattagca catccgactc
     3421 agcttcctcc caaccgacaa tttgcctttt ggtatctgga ctgtcggcat cctcggtctt
     3481 aattttttta tcaggcattg cggctgattg atggcgaata tcccgaaata tgtagccaac
     3541 tgcctattgg gtgttatgtt ggaactactt tgaaaatgtg ctattagacg cgatgttagt
     3601 gaatcaagtg cggcgaggaa gaataggggt ggatcgggcg tattgaggtt cgacggaccc
     3661 aaatcgtctg cgattataat gagggagggc cagaagcttg gcactggccg tcgttttaca
     3721 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     3781 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     3841 cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct
     3901 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     3961 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttggtccatt
     4021 tgtatgtcca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
     4081 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     4141 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     4201 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     4261 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     4321 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     4381 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     4441 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     4501 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     4561 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     4621 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     4681 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     4741 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     4801 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     4861 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     4921 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     4981 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     5041 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     5101 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     5161 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     5221 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     5281 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     5341 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     5401 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     5461 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     5521 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     5581 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     5641 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     5701 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     5761 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     5821 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     5881 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     5941 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     6001 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     6061 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     6121 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     6181 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     6241 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     6301 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     6361 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     6421 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     6481 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     6541 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     6601 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     6661 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     6721 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     6781 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     6841 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     6901 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     6961 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     7021 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     7081 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     7141 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     7201 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     7261 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     7321 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     7381 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     7441 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     7501 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     7561 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     7621 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     7681 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     7741 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     7801 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     7861 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     7921 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     7981 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     8041 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     8101 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     8761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8941 cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     9001 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     9061 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     9121 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     9181 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     9241 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     9301 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     9361 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     9421 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     9481 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     9541 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     9601 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     9661 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
     9721 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
     9781 ttatttcctt cctcttttct atagtattta aagatacccc aagaagctaa ttataacaag
     9841 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
     9901 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
     9961 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
    10021 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
    10081 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
    10141 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
    10201 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
    10261 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
    10321 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
    10381 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
    10441 ccttatctgg gaactactca cacattatta tggagaaa
//
