LOCUS       sequence-232160-       14847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ptet-on::cexA::ttrpC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14847)
  AUTHORS   Steiger MG, Rassinger A, Mattanovich D, Sauer M
  TITLE     Engineering of the citrate exporter protein enables high citric acid
            production in Aspergillus niger.
  JOURNAL   Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. 
            Epub 2018 Dec 13.
  PUBMED    30553933
REFERENCE   2  (bases 1 to 14847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-03"; volume: "52:224-231. doi"; pages: " 
            10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13"
COMMENT     Sequence Label: pMST-713
FEATURES             Location/Qualifiers
     source          1..14847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          639..754
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(772..1389,1390..1395,1396..1431,1432..1434,1435..1470,
                     1471..1473,1474..1509,1510..1518)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    join(1866..1884,1885..1907,1908..1926,1927..1949,1950..1968,
                     1969..1991,1992..2010,2011..2033,2034..2052,2053..2075,
                     2076..2094,2095..2117,2118..2136)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          2206..2321
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     terminator      3920..4693
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      9744..9753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10337..11362
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10337..11362
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(12025..12041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12025..12041)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      12089..12120
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        12121..12223
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             12224..13039
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(12297..12316)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      13063..13090
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(13102..13689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13182..13201)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13729..13746
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13730..13746
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cagtcaatgg caaggaaact ggagcgcaaa aaaccccgcc cctgacaggg cggggttttt
       61 tcgcgatcgg agtcgataag cttcggagaa tatggagctt catcgaatca ccggcagtaa
      121 gcgaaggaga atgtgaagcc aggggtgtat agccgtcggc gaaatagcat gccattaacc
      181 taggtacaga agtccaattg cttccgatct ggtaaaagat tcacgagata gtaccttctc
      241 cgaagtaggt agagcgagta cccggcgcgt aagctcccta attggcccat ccggcatctg
      301 tagggcgtcc aaatatcgtg cctctcctgc tttgcccggt gtatgaaacc ggaaaggccg
      361 ctcaggagct ggccagcggc gcagaccggg aacacaagct ggcagtcgac ccatccggtg
      421 ctctgcactc gacctgctga ggtccctcag tccctggtag gcagctttgc cccgtctgtc
      481 cgcccggtgt gtcggcgggg ttgacaaggt cgttgcgtca gtccaacatt tgttgccata
      541 ttttcctgct ctccccacca gctgctcttt tcttttctct ttcttttccc atcttcagta
      601 tattcatctt cccatccaag aacctttatt tcccctaagt aagtactttg ctacatccat
      661 actccatcct tcccatccct tattcctttg aacctttcag ttcgagcttt cccacttcat
      721 cgcagcttga ctaacagcta ccccgcttga gcagacatca ccgaattcac catgtctaga
      781 ctggacaaga gcaaagtcat aaacggcgct ctggaattac tcaatggagt cggtatcgaa
      841 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
      901 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
      961 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     1021 aagtcattcc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     1081 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     1141 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     1201 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     1261 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     1321 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     1381 gaaagcggcg ggccggccga cgcccttgac gattttgact tagacatgct cccagccgat
     1441 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     1501 gacatgctcc ccgggtaact aagtaaggat ccactagtac agcagaagaa tctctctccg
     1561 ctgttgcttc agtgtctgcc atgcattaac ttcatcctac tgtcctaccc gcagtaccca
     1621 ttcacatttg cggcagatac caggttgttt tctattccct cggttcttca gttcttcaga
     1681 tattacatta ctcgagaaat tggcggcgat gcaggagttt ggttggtttt aattgtttca
     1741 gtctccttga cctgatttgt atgacatgca atgtttcgac ggatgactat ctcggtgaat
     1801 atacgtcatg aatcatgcgg ccgcgcgtat cacgaggccc tttcgacttc actcgagttt
     1861 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     1921 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctccccatc
     2161 ttcagtatat tcatcttccc atccaagaac ctttatttcc cctaagtaag tactttgcta
     2221 catccatact ccatccttcc catcccttat tcctttgaac ctttcagttc gagctttccc
     2281 acttcatcgc agcttgacta acagctaccc cgcttgagca gacatcaccg tttaaacacc
     2341 atgtcctcaa ccacgtcctc atcaagatca gaccttgaaa aggtccccgt accacaggtc
     2401 acccctagag acagtgactc cgataaggga tccctctctc cggagccttc gaccctcgag
     2461 gctcagtcat ccgagaagcc accgcatcat atcttcacac gatctcgcaa gctgcaaatg
     2521 gtttgcatcg tctccctcgc tgccatattt tctccgcttt cgtcgaacat ttacttccct
     2581 gccctggatg atgtctcgaa atccctcaac atcagcatgt cgctcgcaac actcaccatc
     2641 acggtgtaca tgatcgtcca aggcctcgct cccagcttct ggggttccat gtcagacgcc
     2701 acaggtagac ggcctgtctt tattggaaca ttcattgttt acctcgtagc caatattgct
     2761 ctggccgaat ccaagaacta tggtgagctc atggccttcc gagccttgca ggctgctggt
     2821 agcgcagcca ccatctcaat cggagctgga gtgattggtg atatcacaaa ctcggaagaa
     2881 agaggtagct tggtgggtat cttcggtgga gttcgcatgc ttggacaggg aatcgggccg
     2941 gttttcggcg gcattttcac ccagtatctc ggatatcgat ctatcttttg gttcctcacg
     3001 attgctggag gcgtgagtct cctgtccatt ctggtgcttc ttccggagac attgaggcca
     3061 attgctggaa atggaactgt gaagctcaat ggcattcata agcccttcat ctacacgatc
     3121 accggccaga cgggggttgt cgagggagcg caaccggaag cgaaaaagac caaaaccagc
     3181 tggaagtctg tttttgctcc tttgacattc ctcgtcgaaa aggacgtttt catcaccctg
     3241 ttctttggaa gtatcgtgta cacagtgtgg agcatggtga catccagtac caccgacctc
     3301 ttcagcgaag tgtacggcct gtcatccctg gacattggac tcactttcct aggcaatggc
     3361 tttggatgta tgtctggctc ttatctggtc ggctacctta tggattacaa ccaccgtctt
     3421 accgaacgcg aatattgcga gaaacacggt tatccggcag gcacacgtgt caatctgaaa
     3481 tcacaccccg acttccccat tgaggtcgcc cggatgcgca atacctggtg ggtgattgcg
     3541 atcttcatcg tgacagttgc tttgtacggc gtgtctttgc ggacacatct ggcggtgcct
     3601 atcattctgc agtacttcat tgcgttctgc tcaacaggac tcttcaccat caacagcgcc
     3661 ctggtcatcg atctttaccc aggtgctagc gccagtgcga cagcagtgaa caatctgatg
     3721 cggtgcctgc ttggagctgg cggtgtggct atcgtgcaac ctatcctgga cgccttgaag
     3781 ccggattata ctttcctctt gcttgccggc atcacccttg tgatgactcc gttgctgtac
     3841 gtcgaagatc gatggggtcc tggctggcga catgcccgcg aaaggagact caaggccaaa
     3901 gccaacggca actaggcttg atccacttaa cgttactgaa atcatcaaac agcttgacga
     3961 atctggatat aagatcgttg gtgtcgatgt cagctccgga gttgagacaa atggtgttca
     4021 ggatctcgat aagatacgtt catttgtcca agcagcaaag agtgccttct agtgatttaa
     4081 tagctccatg tcaacaagaa taaaacgcgt ttcgggttta cctcttccag atacagctca
     4141 tctgcaatgc attaatgcat tggacctcgc aaccctagta cgcccttcag gctccggcga
     4201 agcagaagaa tagcttagca gagtctattt tcattttcgg gagacgagat caagcagatc
     4261 aacggtcgtc aagagtccta cgagactgag gaatccgctc ttggctccac gcgactatat
     4321 atttgtctct aattgtactt tgacatgctc ctcttcttta ctctgatagc ttgactatga
     4381 aaattccgtc accagcccct gggttcgcaa agataattgc actgtttctt ccttgaactc
     4441 tcaagcctac aggacacaca ttcatcgtag gtataaacct cgaaaatcat tcctactaag
     4501 atgggtatac aatagtaacc atgcatggtt gcctagtgaa tgctccgtaa cacccaatac
     4561 gccggccgaa acttttttac aactctccta tgagtcgttt acccagaatg cacaggtaca
     4621 cttgtttaga ggtaatcctt ctttctagaa gtcctcgtgt actgtgtaag cgcccactcc
     4681 acatctccac tcgctccggg gagacgggca caaggttcat gaaccgaaga ggatgggaac
     4741 cccgaccatg gcctgcggcc gcagctatca gtcctctcag ctgacgcccc tcacgaaccc
     4801 tcgcactctc ccgaaatgct cttcggtgtc catggcgcat catctcagca gcatcccatg
     4861 tcaaaccatg gctttgggcc cacggactct gtggccctgc cgcaacatca ccatcaccat
     4921 cgactcccgc cccatgcagc gctgcgcgcc ccagggcgtc atggagtgaa tgtggaatct
     4981 cctcctttgc cctcgggccc gccgagtgtg gtgggccagc ctggaatgcc tgatccagcc
     5041 cccaggcctc gaggaccaaa actgaagttt actcccgaag aggacgctct actggtggag
     5101 ttgaaggaaa acaagaactt gacgtggaag caaattgcag acttcttccc gggccgaacg
     5161 agcggtacct tgcaagtccg atactgcacc aagctgaagg ctaaggatgt agcttggagt
     5221 gacgaaatgg ttcgatttgc tcctgatgta tttctacgcc tgtctcacac atgctaatga
     5281 agggaatagg tacaaaggct gcagcgggca atgcacgagt acgagaacga tcggtggcgc
     5341 atcattgcag ggaaggttgg aaatggcttc accccagctg cttgccgcga gaaagccatg
     5401 cagctccatg agtaaaagcg ttggggaatt ttcatattta tatctactgt cgccagattc
     5461 ggccctgctt ggaccctctg atctccttac tctccatatt ggttcaaatg tcgggtcacc
     5521 gatagggctg gtggtgcagg cttgttgtag gcacgggagg atgatcagca taactctgag
     5581 tcactatagg gacgggttga tgtaaggtat taagtgatgt atgataattc attttagccc
     5641 gggggaacat atggcgccgg catttgttcg ttcgcaatga accgacacta gcgtccgctc
     5701 tcgcagttta gcaccggctg atcccgggct gaacgcggcc attgctcggc cggggcatgt
     5761 gttccttatc tacggcagac cgcagatgac cactggagca gattatagac cctaagccct
     5821 aagccggaca cccaatcgag taggtctgcg gaccaggtca ctgcgggcag ccggagaagc
     5881 tccgcaacca atcaatcccc ggcgctgact aagggcaggc gaccacgggc cgaagcggct
     5941 tcaaactcac ctcaacctcc aaactccctc atctccaaac gtccttgcct tgtctgccgt
     6001 cattgcaacc cacccaccag gacacatgag cagcaagtcc cagctcacct acactgcccg
     6061 tgccagcaag catcccaatg ctctggcgaa gaggctgttc gagattgccg aggccaagaa
     6121 aaccaatgtg actgtctcgg ctgacgttac caccactaag gagctactag atcttgctga
     6181 ccgtctcggt ccctacattg ccgtgatcaa aacccacatc gatatcctct ctgatttcag
     6241 caacgagact attgagggac ttaaggctct cgcgcagaag cacaactttc tcatcttcga
     6301 ggaccgcaag ttcattgaca tcggcaacac ggtccagaag caataccacg gcggtaccct
     6361 ccgtatctcg gaatgggccc acatcatcaa ctgcagcatt ctccctggtg agggtatcgt
     6421 cgaggctctc gctcagacgg cgtctgcacc ggacttcgcc tacggccccg aacgcggtct
     6481 gttgatcttg gcagagatga cctctaaggg ctccttggct accggccagt acactacttc
     6541 ctcggtcgat tatgcccgga aatacaagaa cttcgttatg ggattcgtgt cgacgcgcgc
     6601 gttgggtgag gtgcagtcgg aagtcagctc tccttcggat gaggaggact ttgtggtttt
     6661 cacgactggt gtgaacatta acctccaagt cgcaattgac ctacgcttat gctgcagacg
     6721 cgttacgtat cggatccaga attcgtgata tctgaattcg tcgacaagct tattttttgt
     6781 atactgtttt gtgatagcac gaagtttttc cacggtatct tgttaaaaat atatatttgt
     6841 ggcgggctta cctacatcaa attaataaga gactaattat aaactaaaca cacaagcaag
     6901 ctactttagg gtaaaagttt ataaatgctt ttgacgtata aacgttgctt gtatttatta
     6961 ttacaattaa aggtggatag aaaacctaga gactagttag aaactaatct caggtttgcg
     7021 ttaaactaaa tcagagcccg agaggttaac agaacctaga aggggactag atatccgggt
     7081 agggaaacaa aaaaaaaaaa caagacagcc acatattagg gagactagtt agaagctagt
     7141 tccaggacta ggaaaataaa agacaatgat accacagtct agttgacaac tagatagatt
     7201 ctagattgag gccaaagtct ctgagatcca ggttagttgc aactaatact agttagtatc
     7261 tagtctccta taactctgaa gctagaataa cttactacta ttatcctcac cactgttcag
     7321 ctgcgcaaac ggagtgattg caaggtgttc agagactagt tattgactag tcagtgacta
     7381 gcaataacta acaaggtatt aacctaccat gtctgccatc accctgcact tcctcgggct
     7441 cagcagcctt ttcctcctca ttttcatgct cattttcctt gtttaagact gtgactagtc
     7501 aaagactagt ccagaaccac aaaggagaaa tgtcttacca ctttcttcat tgcttgtctc
     7561 ttttgcatta tccatgtctg caactagtta gagtctagtt agtgactagt ccgacgagga
     7621 cttgcttgtc tccggattgt tggaggaact ctccagggcc tcaagatcca caacagagcc
     7681 ttctagatga ctggtcaata actagttggt ctttgtctga gtctgactga cttacgaggt
     7741 tgcatactcg ctccctttgc ctcgtcaatc gatgagaaaa agcgccaaaa ctcgcaatat
     7801 ggctttgaac cacacggtgc tgagactagt tagaatctag tcccaaacta gcttggatag
     7861 cttacctttg ccctttgcgt tgcgacaggt cttgcagggt atggttcctt tctcaccagc
     7921 tgatttagct gccttgctac cctcacggcg gatctgcata aagagtggct agaggttata
     7981 aattagcact gatcctaggt acggggctga atgtaacttg cctttccttt ctcatcgcgc
     8041 ggcaagacag gcttgctcaa attcctacca gtcacagggg tatgcacggc gtacggacca
     8101 cttgaactag tcacagatta gttagcaact agtctgcatt gaatggctgt acttacgggc
     8161 cctcgccatt gtcctgatca tttccagctt caccctcgtt gctgcaaagt agttagtgac
     8221 tagtcaagga ctagttgaaa tgggagaaga aactcacgaa ttctcgacac ccttagtatt
     8281 gtggtccttg gacttggtgc tgctatatat tagctaatac actagttaga ctcacagaaa
     8341 cttacgcagc tcgcttgcgc ttcttggtag gagtcggggt tgggagaaca gtgccttcaa
     8401 acaagccttc ataccatgct acttgactag tcagggacta gtcaccaagt aatctagata
     8461 ggacttgcct ttggcctcca tcagttcctt catagtggga ggtccattgt gcaatgtaaa
     8521 ctccatgccg tgggagttct tgtccttcaa gtgcttgacc aatatgtttc tgttggcaga
     8581 gggaacctgt caactagtta ataactagtc agaaactagt atagcagtag actcactgta
     8641 cgcttgaggc atcccttcac tcggcagtag acttcatatg gatggatatc aggcacgcca
     8701 ttgtcgtcct gtggactagt cagtaactag gcttaaagct agtcgggtcg gcttactatc
     8761 ttgaaatccg gcagcgtaag ctccccgtcc ttaactgcct cgagatagtg acagtactct
     8821 ggggactttc ggagatcgtt atcgcgaatg ctcggcatac taatcgttga ctagtcttgg
     8881 actagtcccg agcaaaaagg attggaggag gaggaggaag gtgagagtga gacaaagagc
     8941 gaaataagag cttcaaaggc tatctctaag cagtatgaag gttaagtatc tagttcttga
     9001 ctagatttaa aagagatttc gactagttat gtacctggag tttggatata ggaatgtgtt
     9061 gtggtaacga aatgtaaggg ggaggaaaga aaaagtcggt caagaggtaa ctctaagtcg
     9121 gccattcctt tttgggaggc gctaaccata aacggcatgg tcgacttaga gttagctcag
     9181 ggaatttagg gagttatctg cgaccaccga ggaacggcgg aatgccaaag aatcccgatg
     9241 gagctctagc tggcggttga caaccccacc ttttggcgtt tctgcggcgt tgcaggcggg
     9301 actggatact tcgtagaacc agaaaggcaa ggcagaacgc gctcagcaag agtgttggaa
     9361 gtgatagcat gatgtgcctt gttaactagg tcaaaatctg cagtatgctt gatgttatcc
     9421 aaagtgtgag agaggaaggt ccaaacatac acgattggga gagggcctag gtataagagt
     9481 ttttgagtag aacgcatgtg agcccagcca tctcgaggag attaaacacg ggccggcatt
     9541 tgatggctat gttagtaccc caatggaaag cctgagagtc cagtggcgct aattcatgaa
     9601 taacggtgag actagcggcc ggtcccctta tcccagctgt tccacgttgg cctgcccctc
     9661 agttagcgct caactcaatg cccctcactg gcgaggcgag ggcaaggatg gaggggcagc
     9721 atcgcctgag ttggagcaaa gcggccgcca tgggagcagc gaaccaacgg agggatgccg
     9781 tgctttgtcg tggctgctgt ggccaatccg ggcccttggt tggctcacag agcgttgctg
     9841 tgagtccatg agctattatt gctaggtaca gtatagagag aggagagaga gagagagaga
     9901 gagaggggaa aaaaggtgag gttgaagtga gaaaaaaaaa aaaaaaaatc caaccactga
     9961 cggctgccgg ctctgccacc cccctccctc caccccagac cacctgcaca ctcagcgcgc
    10021 agcatcacct aatcttggct cgccttcccg cagctcaggt tgtttttttt ttctctctcc
    10081 ctcgtcgaag ccgcccttgt tcccttattt atttccctct ccatccttgt ctgcctttgg
    10141 tccatctgcc cctttgtctg catctctttt gcacgcatcg ccttatcgtc gtctcttttt
    10201 tcactcacgg gagcttgacg atgacctgac tcgtgagcct cacctgctga tttctctccc
    10261 cccctcccga ccggcttgac ttttgtttct cctccagtac cttatcgcga agccggaaga
    10321 acctcttaac ctctagatga aaaagcctga actcaccgcg acgtctgtcg agaagttcct
    10381 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
    10441 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
    10501 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
    10561 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
    10621 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
    10681 cgcggaggcc atggatgcga tcgctgcggc cgatctcagc cagacgagcg ggttcggccc
    10741 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
    10801 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
    10861 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
    10921 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
    10981 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
    11041 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcacccgga
    11101 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
    11161 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
    11221 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
    11281 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
    11341 tcgtccgagg gcaaaggaat agatgcatgg ctttcgtgac cgggcttcaa acaatgatgt
    11401 gcgatggtgt ggttcccggt tggcggagtc tttgtctact ttggttgtct gtcgcaggtc
    11461 ggtagaccgc aaatgagcaa ctgatggatt gttgccagcg atactataat tcacatggat
    11521 ggtctttgtc gatcagtagc tagtgagaga gagagaacat ctatccacaa tgtcgagtgt
    11581 ctattagaca tactccgaga ataaagtcaa ctgtgtctgt gatctaaaga tcgattcggc
    11641 agtcgagtag cgtataacaa ctccgagtac cagcgaaagc acgtcgtgac aggagcaggg
    11701 ctttgccaac tgcgcaacct tgcttgaatg aggatacacg gggtgcaaca tggctgtact
    11761 gatccatcgc aaccaaaatt tctgtttata gatcaagctg gtagattcca attactccac
    11821 ctcttgcgct tctccatgac atgtaagtgc acgtggaaac catacccaaa ttgcctacag
    11881 ctgcggagca tgagcctatg gcgatcagtc tggtcatgtt aaccagcctg tgctctgacg
    11941 ttaatgcaga atagcttcgc gtgatcttac ggcattatac gtatgatcgg tccacgatca
    12001 gctagattat ctagtcagct tgatgtcata gctgtttcct gaggctcaat actgaccatt
    12061 taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag gcttttgact
    12121 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
    12181 attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg
    12241 ggaaacgtct tgcttgaagc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
    12301 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
    12361 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
    12421 agatgagatg gtcaggctaa actggctgac ggaatttatg cctcttccga ccatcaagca
    12481 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatcccag ggaaaacagc
    12541 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
    12601 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaacg gcgatcgcgt
    12661 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttggtg cgagtgattt
    12721 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactctt
    12781 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
    12841 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
    12901 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
    12961 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcacttgat
    13021 gctcgatgag tttttctaat gaggacctaa atgtaatcac ctggctcacc ttcgggtggg
    13081 cctttctgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    13141 tcgatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    13201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    13261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    13321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    13381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    13441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    13501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    13561 cgctctgctg aagccagtta cctcggaaaa agagttggta gctcttgatc cggcaaacaa
    13621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    13681 ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg taaaacgacg
    13741 gccagtttat ctagtcagct tgattctagc tgatcgtgga ccggaaggtg agccagtgag
    13801 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat gcggcccgct
    13861 gtaggtcaat tgcgacttgg aggaagggat ccgccaagga tgctctggat ccgcatccgg
    13921 ccgctcttgc gccccatcaa tcgcccgact ataaatcgaa ctactttcgg catcttctag
    13981 acttcctaat accgcctagt catagcagat tcaagctgag aacaccacaa gtaaatatca
    14041 cccatcatgc ttaccctgac cgtccctgaa aactacgggt atgtgccaat tctacaattc
    14101 cttgcagaca atgccattct ccccatgaag tctgatgcta actatcctgc agctctgtca
    14161 ttgccgtcgc tctgggtgcc atccccgtcc tgagcttcgt ccatggcgcc gtcgtgtctc
    14221 gtctccgcaa ggaagctgat tgcccctacc ctcactgcta tgcgaccgta gagcagtgca
    14281 agaccaacgt aagccaacct cacacaaaca ggattcctcg agctaacata cattccgaac
    14341 cgtgcagccc aaggccgagc agttcaactg cgctcagcgc gctcatgcca acttccttga
    14401 gaactccagc caaactatgc tcttcctcct ggtagctgga ctgaagtacc cccagttggc
    14461 gactggcctc ggaagcatct gggtcctcgg tcgctcactg ttcctttacg gatatgtgta
    14521 ctccggcaag ccgcggggtc gcggtcgttt gtacggcagc ttctacttgc ttgcacaggg
    14581 agctctctgg ggcttgacgt cttttggagt tgcgagggag ttgatttcct acttctaagt
    14641 ttggactgaa tccgtggtgt gattgaggtg attggcgatg tttggctata ccagctatat
    14701 gtaataatct ctactgtata ctactattca acgcatttta ctatgcgtgc tgctagggtc
    14761 ggcaatgaca atggcaatct gactgacgtg gtctatttct ccatgtgcag cagggaatac
    14821 gagctccaat ggacctcggg agtggca
//
