LOCUS       sequence-232161-       14082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pmbfA::cexA::ttrpC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14082)
  AUTHORS   Steiger MG, Rassinger A, Mattanovich D, Sauer M
  TITLE     Engineering of the citrate exporter protein enables high citric acid
            production in Aspergillus niger.
  JOURNAL   Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. 
            Epub 2018 Dec 13.
  PUBMED    30553933
REFERENCE   2  (bases 1 to 14082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-03"; volume: "52:224-231. doi"; pages: " 
            10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13"
COMMENT     Sequence Label: pMST-712
FEATURES             Location/Qualifiers
     source          1..14082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      3155..3928
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     regulatory      8979..8988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9572..10597
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9572..10597
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(11260..11276)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11260..11276)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      11324..11355
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        11356..11458
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             11459..12274
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(11532..11551)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      12298..12325
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(12337..12924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12417..12436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12964..12981
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12965..12981
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cagtcaatgg caaggaaact ggagcgcaaa aaaccccgcc cctgacaggg cggggttttt
       61 tcgcgatcgg aggtacagtg gccatgaaat ccaatcattt ccttctggcc gccctcgggc
      121 aagagatagt gccgcagagg actctcacag catctacatc tgcgaccgca acagccacca
      181 agcgaggcgc acatgagctt gtcctcctcc catgccaaag tttggccctc ttcgtttctg
      241 tgatgctgaa ggaagtcaaa ctcgtcgatg ataggaccag atggtttgtc aagggtcaac
      301 gctttccatg ccttctggca ccggtagtaa tgctcttctg caagggagac ttgacgtttc
      361 ggatccgcgg gccccgggac atgctggaag ggattttctg gctcaatacc acgtctgtat
      421 ttgacccttt ccagacagtt aatccgctgc aggagggcga actgtagctc ctcgttctcc
      481 ttgtagcgct tgatccagtc tttttggatg ttgcacttgc ttggcctatg cttctcatat
      541 aatcttgccc tgtcatagag acgacgtctg agattgtagc gttcgtcttt gatcacccgg
      601 agccagatag gcctgagtat atctgacatt agatcaaagg gtctgtggat agtctccttc
      661 agcatcagcg acgcatgtga ctcgcatgtc ggagagagct tgtgggtggt catctttgat
      721 ggcgtcctct gctttccctt gattttcgtt gattgttttt cgaaagttaa gtctggaagt
      781 caagagaatc cttctgccag acattatatt tacgtatact gacgtagtag aaacagcgtc
      841 aggatgagga catggtgtgt gctggaccac ggaatcatag ttcatcagta tattgggttg
      901 gacaaataac gctgagcatg tatatgtctt tacacactat aaaagccagc gaacgccaat
      961 aaaatagggc atattgatgt gaaaatatga caccagttaa aagcagtgta ttgattttat
     1021 ctctcttcac ctcggaccta tactaccgta tacaagactc aacttacttc cagatatagt
     1081 aatatacacc ctatggacga accagcacaa taattacagc caaacaacac cacccaaatg
     1141 gcatattcct aatcagcact aagcacaaat accactgtca tcacagcata atcaataaga
     1201 atcccagaca accgactcac tctgactcac cttacacaaa cccccaagca aagcgcagcc
     1261 cagaacctca gccaacaatc gggcaacgta cggggaaaga ttggccgatc catgatgtca
     1321 gcagccctaa cccaaagcgg actagcgcat accgcccctc tgactccgcc atcccagggc
     1381 tcgagaagct tccgtggcgt cgatataaat tcagcgggcc ttgaacatcc ctccttacga
     1441 cacacctcac gcgatcgatt ttgacactca cacaccgcca ccctcacatc ctccacccac
     1501 accacacccc ttaatcaacc caccatcacc gctagaacgt ctatctcatc accgacttct
     1561 catccatctt caaacatgtc ctcaaccacg tcctcatcaa gatcagacct tgaaaaggtc
     1621 cccgtaccac aggtcacccc tagagacagt gactccgata agggatccct ctctccggag
     1681 ccttcgaccc tcgaggctca gtcatccgag aagccaccgc atcatatctt cacacgatct
     1741 cgcaagctgc aaatggtttg catcgtctcc ctcgctgcca tattttctcc gctttcgtcg
     1801 aacatttact tccctgccct ggatgatgtc tcgaaatccc tcaacatcag catgtcgctc
     1861 gcaacactca ccatcacggt gtacatgatc gtccaaggcc tcgctcccag cttctggggt
     1921 tccatgtcag acgccacagg tagacggcct gtctttattg gaacattcat tgtttacctc
     1981 gtagccaata ttgctctggc cgaatccaag aactatggtg agctcatggc cttccgagcc
     2041 ttgcaggctg ctggtagcgc agccaccatc tcaatcggag ctggagtgat tggtgatatc
     2101 acaaactcgg aagaaagagg tagcttggtg ggtatcttcg gtggagttcg catgcttgga
     2161 cagggaatcg ggccggtttt cggcggcatt ttcacccagt atctcggata tcgatctatc
     2221 ttttggttcc tcacgattgc tggaggcgtg agtctcctgt ccattctggt gcttcttccg
     2281 gagacattga ggccaattgc tggaaatgga actgtgaagc tcaatggcat tcataagccc
     2341 ttcatctaca cgatcaccgg ccagacgggg gttgtcgagg gagcgcaacc ggaagcgaaa
     2401 aagaccaaaa ccagctggaa gtctgttttt gctcctttga cattcctcgt cgaaaaggac
     2461 gttttcatca ccctgttctt tggaagtatc gtgtacacag tgtggagcat ggtgacatcc
     2521 agtaccaccg acctcttcag cgaagtgtac ggcctgtcat ccctggacat tggactcact
     2581 ttcctaggca atggctttgg atgtatgtct ggctcttatc tggtcggcta ccttatggat
     2641 tacaaccacc gtcttaccga acgcgaatat tgcgagaaac acggttatcc ggcaggcaca
     2701 cgtgtcaatc tgaaatcaca ccccgacttc cccattgagg tcgcccggat gcgcaatacc
     2761 tggtgggtga ttgcgatctt catcgtgaca gttgctttgt acggcgtgtc tttgcggaca
     2821 catctggcgg tgcctatcat tctgcagtac ttcattgcgt tctgctcaac aggactcttc
     2881 accatcaaca gcgccctggt catcgatctt tacccaggtg ctagcgccag tgcgacagca
     2941 gtgaacaatc tgatgcggtg cctgcttgga gctggcggtg tggctatcgt gcaacctatc
     3001 ctggacgcct tgaagccgga ttatactttc ctcttgcttg ccggcatcac ccttgtgatg
     3061 actccgttgc tgtacgtcga agatcgatgg ggtcctggct ggcgacatgc ccgcgaaagg
     3121 agactcaagg ccaaagccaa cggcaactag gcttgatcca cttaacgtta ctgaaatcat
     3181 caaacagctt gacgaatctg gatataagat cgttggtgtc gatgtcagct ccggagttga
     3241 gacaaatggt gttcaggatc tcgataagat acgttcattt gtccaagcag caaagagtgc
     3301 cttctagtga tttaatagct ccatgtcaac aagaataaaa cgcgtttcgg gtttacctct
     3361 tccagataca gctcatctgc aatgcattaa tgcattggac ctcgcaaccc tagtacgccc
     3421 ttcaggctcc ggcgaagcag aagaatagct tagcagagtc tattttcatt ttcgggagac
     3481 gagatcaagc agatcaacgg tcgtcaagag tcctacgaga ctgaggaatc cgctcttggc
     3541 tccacgcgac tatatatttg tctctaattg tactttgaca tgctcctctt ctttactctg
     3601 atagcttgac tatgaaaatt ccgtcaccag cccctgggtt cgcaaagata attgcactgt
     3661 ttcttccttg aactctcaag cctacaggac acacattcat cgtaggtata aacctcgaaa
     3721 atcattccta ctaagatggg tatacaatag taaccatgca tggttgccta gtgaatgctc
     3781 cgtaacaccc aatacgccgg ccgaaacttt tttacaactc tcctatgagt cgtttaccca
     3841 gaatgcacag gtacacttgt ttagaggtaa tccttctttc tagaagtcct cgtgtactgt
     3901 gtaagcgccc actccacatc tccactcgct ccggggagac gggcacaagg ttcatgaacc
     3961 gaagaggatg ggaaccccga ccatggcctg cggccgcagc tatcagtcct ctcagctgac
     4021 gcccctcacg aaccctcgca ctctcccgaa atgctcttcg gtgtccatgg cgcatcatct
     4081 cagcagcatc ccatgtcaaa ccatggcttt gggcccacgg actctgtggc cctgccgcaa
     4141 catcaccatc accatcgact cccgccccat gcagcgctgc gcgccccagg gcgtcatgga
     4201 gtgaatgtgg aatctcctcc tttgccctcg ggcccgccga gtgtggtggg ccagcctgga
     4261 atgcctgatc cagcccccag gcctcgagga ccaaaactga agtttactcc cgaagaggac
     4321 gctctactgg tggagttgaa ggaaaacaag aacttgacgt ggaagcaaat tgcagacttc
     4381 ttcccgggcc gaacgagcgg taccttgcaa gtccgatact gcaccaagct gaaggctaag
     4441 gatgtagctt ggagtgacga aatggttcga tttgctcctg atgtatttct acgcctgtct
     4501 cacacatgct aatgaaggga ataggtacaa aggctgcagc gggcaatgca cgagtacgag
     4561 aacgatcggt ggcgcatcat tgcagggaag gttggaaatg gcttcacccc agctgcttgc
     4621 cgcgagaaag ccatgcagct ccatgagtaa aagcgttggg gaattttcat atttatatct
     4681 actgtcgcca gattcggccc tgcttggacc ctctgatctc cttactctcc atattggttc
     4741 aaatgtcggg tcaccgatag ggctggtggt gcaggcttgt tgtaggcacg ggaggatgat
     4801 cagcataact ctgagtcact atagggacgg gttgatgtaa ggtattaagt gatgtatgat
     4861 aattcatttt agcccggggg aacatatggc gccggcattt gttcgttcgc aatgaaccga
     4921 cactagcgtc cgctctcgca gtttagcacc ggctgatccc gggctgaacg cggccattgc
     4981 tcggccgggg catgtgttcc ttatctacgg cagaccgcag atgaccactg gagcagatta
     5041 tagaccctaa gccctaagcc ggacacccaa tcgagtaggt ctgcggacca ggtcactgcg
     5101 ggcagccgga gaagctccgc aaccaatcaa tccccggcgc tgactaaggg caggcgacca
     5161 cgggccgaag cggcttcaaa ctcacctcaa cctccaaact ccctcatctc caaacgtcct
     5221 tgccttgtct gccgtcattg caacccaccc accaggacac atgagcagca agtcccagct
     5281 cacctacact gcccgtgcca gcaagcatcc caatgctctg gcgaagaggc tgttcgagat
     5341 tgccgaggcc aagaaaacca atgtgactgt ctcggctgac gttaccacca ctaaggagct
     5401 actagatctt gctgaccgtc tcggtcccta cattgccgtg atcaaaaccc acatcgatat
     5461 cctctctgat ttcagcaacg agactattga gggacttaag gctctcgcgc agaagcacaa
     5521 ctttctcatc ttcgaggacc gcaagttcat tgacatcggc aacacggtcc agaagcaata
     5581 ccacggcggt accctccgta tctcggaatg ggcccacatc atcaactgca gcattctccc
     5641 tggtgagggt atcgtcgagg ctctcgctca gacggcgtct gcaccggact tcgcctacgg
     5701 ccccgaacgc ggtctgttga tcttggcaga gatgacctct aagggctcct tggctaccgg
     5761 ccagtacact acttcctcgg tcgattatgc ccggaaatac aagaacttcg ttatgggatt
     5821 cgtgtcgacg cgcgcgttgg gtgaggtgca gtcggaagtc agctctcctt cggatgagga
     5881 ggactttgtg gttttcacga ctggtgtgaa cattaacctc caagtcgcaa ttgacctacg
     5941 cttatgctgc agacgcgtta cgtatcggat ccagaattcg tgatatctga attcgtcgac
     6001 aagcttattt tttgtatact gttttgtgat agcacgaagt ttttccacgg tatcttgtta
     6061 aaaatatata tttgtggcgg gcttacctac atcaaattaa taagagacta attataaact
     6121 aaacacacaa gcaagctact ttagggtaaa agtttataaa tgcttttgac gtataaacgt
     6181 tgcttgtatt tattattaca attaaaggtg gatagaaaac ctagagacta gttagaaact
     6241 aatctcaggt ttgcgttaaa ctaaatcaga gcccgagagg ttaacagaac ctagaagggg
     6301 actagatatc cgggtaggga aacaaaaaaa aaaaacaaga cagccacata ttagggagac
     6361 tagttagaag ctagttccag gactaggaaa ataaaagaca atgataccac agtctagttg
     6421 acaactagat agattctaga ttgaggccaa agtctctgag atccaggtta gttgcaacta
     6481 atactagtta gtatctagtc tcctataact ctgaagctag aataacttac tactattatc
     6541 ctcaccactg ttcagctgcg caaacggagt gattgcaagg tgttcagaga ctagttattg
     6601 actagtcagt gactagcaat aactaacaag gtattaacct accatgtctg ccatcaccct
     6661 gcacttcctc gggctcagca gccttttcct cctcattttc atgctcattt tccttgttta
     6721 agactgtgac tagtcaaaga ctagtccaga accacaaagg agaaatgtct taccactttc
     6781 ttcattgctt gtctcttttg cattatccat gtctgcaact agttagagtc tagttagtga
     6841 ctagtccgac gaggacttgc ttgtctccgg attgttggag gaactctcca gggcctcaag
     6901 atccacaaca gagccttcta gatgactggt caataactag ttggtctttg tctgagtctg
     6961 actgacttac gaggttgcat actcgctccc tttgcctcgt caatcgatga gaaaaagcgc
     7021 caaaactcgc aatatggctt tgaaccacac ggtgctgaga ctagttagaa tctagtccca
     7081 aactagcttg gatagcttac ctttgccctt tgcgttgcga caggtcttgc agggtatggt
     7141 tcctttctca ccagctgatt tagctgcctt gctaccctca cggcggatct gcataaagag
     7201 tggctagagg ttataaatta gcactgatcc taggtacggg gctgaatgta acttgccttt
     7261 cctttctcat cgcgcggcaa gacaggcttg ctcaaattcc taccagtcac aggggtatgc
     7321 acggcgtacg gaccacttga actagtcaca gattagttag caactagtct gcattgaatg
     7381 gctgtactta cgggccctcg ccattgtcct gatcatttcc agcttcaccc tcgttgctgc
     7441 aaagtagtta gtgactagtc aaggactagt tgaaatggga gaagaaactc acgaattctc
     7501 gacaccctta gtattgtggt ccttggactt ggtgctgcta tatattagct aatacactag
     7561 ttagactcac agaaacttac gcagctcgct tgcgcttctt ggtaggagtc ggggttggga
     7621 gaacagtgcc ttcaaacaag ccttcatacc atgctacttg actagtcagg gactagtcac
     7681 caagtaatct agataggact tgcctttggc ctccatcagt tccttcatag tgggaggtcc
     7741 attgtgcaat gtaaactcca tgccgtggga gttcttgtcc ttcaagtgct tgaccaatat
     7801 gtttctgttg gcagagggaa cctgtcaact agttaataac tagtcagaaa ctagtatagc
     7861 agtagactca ctgtacgctt gaggcatccc ttcactcggc agtagacttc atatggatgg
     7921 atatcaggca cgccattgtc gtcctgtgga ctagtcagta actaggctta aagctagtcg
     7981 ggtcggctta ctatcttgaa atccggcagc gtaagctccc cgtccttaac tgcctcgaga
     8041 tagtgacagt actctgggga ctttcggaga tcgttatcgc gaatgctcgg catactaatc
     8101 gttgactagt cttggactag tcccgagcaa aaaggattgg aggaggagga ggaaggtgag
     8161 agtgagacaa agagcgaaat aagagcttca aaggctatct ctaagcagta tgaaggttaa
     8221 gtatctagtt cttgactaga tttaaaagag atttcgacta gttatgtacc tggagtttgg
     8281 atataggaat gtgttgtggt aacgaaatgt aagggggagg aaagaaaaag tcggtcaaga
     8341 ggtaactcta agtcggccat tcctttttgg gaggcgctaa ccataaacgg catggtcgac
     8401 ttagagttag ctcagggaat ttagggagtt atctgcgacc accgaggaac ggcggaatgc
     8461 caaagaatcc cgatggagct ctagctggcg gttgacaacc ccaccttttg gcgtttctgc
     8521 ggcgttgcag gcgggactgg atacttcgta gaaccagaaa ggcaaggcag aacgcgctca
     8581 gcaagagtgt tggaagtgat agcatgatgt gccttgttaa ctaggtcaaa atctgcagta
     8641 tgcttgatgt tatccaaagt gtgagagagg aaggtccaaa catacacgat tgggagaggg
     8701 cctaggtata agagtttttg agtagaacgc atgtgagccc agccatctcg aggagattaa
     8761 acacgggccg gcatttgatg gctatgttag taccccaatg gaaagcctga gagtccagtg
     8821 gcgctaattc atgaataacg gtgagactag cggccggtcc ccttatccca gctgttccac
     8881 gttggcctgc ccctcagtta gcgctcaact caatgcccct cactggcgag gcgagggcaa
     8941 ggatggaggg gcagcatcgc ctgagttgga gcaaagcggc cgccatggga gcagcgaacc
     9001 aacggaggga tgccgtgctt tgtcgtggct gctgtggcca atccgggccc ttggttggct
     9061 cacagagcgt tgctgtgagt ccatgagcta ttattgctag gtacagtata gagagaggag
     9121 agagagagag agagagagag gggaaaaaag gtgaggttga agtgagaaaa aaaaaaaaaa
     9181 aaatccaacc actgacggct gccggctctg ccacccccct ccctccaccc cagaccacct
     9241 gcacactcag cgcgcagcat cacctaatct tggctcgcct tcccgcagct caggttgttt
     9301 tttttttctc tctccctcgt cgaagccgcc cttgttccct tatttatttc cctctccatc
     9361 cttgtctgcc tttggtccat ctgccccttt gtctgcatct cttttgcacg catcgcctta
     9421 tcgtcgtctc ttttttcact cacgggagct tgacgatgac ctgactcgtg agcctcacct
     9481 gctgatttct ctccccccct cccgaccggc ttgacttttg tttctcctcc agtaccttat
     9541 cgcgaagccg gaagaacctc ttaacctcta gatgaaaaag cctgaactca ccgcgacgtc
     9601 tgtcgagaag ttcctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     9661 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     9721 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     9781 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     9841 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     9901 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc tcagccagac
     9961 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
    10021 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
    10081 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
    10141 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
    10201 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
    10261 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
    10321 gcggaggcac ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
    10381 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
    10441 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
    10501 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
    10561 ccgacgcccc agcactcgtc cgagggcaaa ggaatagatg catggctttc gtgaccgggc
    10621 ttcaaacaat gatgtgcgat ggtgtggttc ccggttggcg gagtctttgt ctactttggt
    10681 tgtctgtcgc aggtcggtag accgcaaatg agcaactgat ggattgttgc cagcgatact
    10741 ataattcaca tggatggtct ttgtcgatca gtagctagtg agagagagag aacatctatc
    10801 cacaatgtcg agtgtctatt agacatactc cgagaataaa gtcaactgtg tctgtgatct
    10861 aaagatcgat tcggcagtcg agtagcgtat aacaactccg agtaccagcg aaagcacgtc
    10921 gtgacaggag cagggctttg ccaactgcgc aaccttgctt gaatgaggat acacggggtg
    10981 caacatggct gtactgatcc atcgcaacca aaatttctgt ttatagatca agctggtaga
    11041 ttccaattac tccacctctt gcgcttctcc atgacatgta agtgcacgtg gaaaccatac
    11101 ccaaattgcc tacagctgcg gagcatgagc ctatggcgat cagtctggtc atgttaacca
    11161 gcctgtgctc tgacgttaat gcagaatagc ttcgcgtgat cttacggcat tatacgtatg
    11221 atcggtccac gatcagctag attatctagt cagcttgatg tcatagctgt ttcctgaggc
    11281 tcaatactga ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac
    11341 cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag
    11401 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
    11461 gagccatatt caacgggaaa cgtcttgctt gaagccgcga ttaaattcca acatggatgc
    11521 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
    11581 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
    11641 tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct
    11701 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
    11761 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
    11821 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
    11881 taacggcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
    11941 tggtgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
    12001 aatgcataaa ctcttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
    12061 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
    12121 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
    12181 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
    12241 gcagtttcac ttgatgctcg atgagttttt ctaatgagga cctaaatgta atcacctggc
    12301 tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc cataggctcc gcccccctga
    12361 cgagcatcac aaaaatcgat gctcaagtca gaggtggcga aacccgacag gactataaag
    12421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    12481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    12541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12781 agtatttggt atctgcgctc tgctgaagcc agttacctcg gaaaaagagt tggtagctct
    12841 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12901 acgcgcagaa aaaaaggatc tcaagaagat cctttgattt tctaccgaag aaaggcccac
    12961 ccgtgtaaaa cgacggccag tttatctagt cagcttgatt ctagctgatc gtggaccgga
    13021 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
    13081 gatatgcggc ccgctgtagg tcaattgcga cttggaggaa gggatccgcc aaggatgctc
    13141 tggatccgca tccggccgct cttgcgcccc atcaatcgcc cgactataaa tcgaactact
    13201 ttcggcatct tctagacttc ctaataccgc ctagtcatag cagattcaag ctgagaacac
    13261 cacaagtaaa tatcacccat catgcttacc ctgaccgtcc ctgaaaacta cgggtatgtg
    13321 ccaattctac aattccttgc agacaatgcc attctcccca tgaagtctga tgctaactat
    13381 cctgcagctc tgtcattgcc gtcgctctgg gtgccatccc cgtcctgagc ttcgtccatg
    13441 gcgccgtcgt gtctcgtctc cgcaaggaag ctgattgccc ctaccctcac tgctatgcga
    13501 ccgtagagca gtgcaagacc aacgtaagcc aacctcacac aaacaggatt cctcgagcta
    13561 acatacattc cgaaccgtgc agcccaaggc cgagcagttc aactgcgctc agcgcgctca
    13621 tgccaacttc cttgagaact ccagccaaac tatgctcttc ctcctggtag ctggactgaa
    13681 gtacccccag ttggcgactg gcctcggaag catctgggtc ctcggtcgct cactgttcct
    13741 ttacggatat gtgtactccg gcaagccgcg gggtcgcggt cgtttgtacg gcagcttcta
    13801 cttgcttgca cagggagctc tctggggctt gacgtctttt ggagttgcga gggagttgat
    13861 ttcctacttc taagtttgga ctgaatccgt ggtgtgattg aggtgattgg cgatgtttgg
    13921 ctataccagc tatatgtaat aatctctact gtatactact attcaacgca ttttactatg
    13981 cgtgctgcta gggtcggcaa tgacaatggc aatctgactg acgtggtcta tttctccatg
    14041 tgcagcaggg aatacgagct ccaatggacc tcgggagtgg ca
//
