LOCUS       sequence-232206-        9616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial luminescence promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9616)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 9616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-Lux-nat
FEATURES             Location/Qualifiers
     source          1..9616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             397..1839
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1852..2775
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2824..3906
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3921..4904
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5083..6195
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     misc_feature    7542..7560
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(7626..7643)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7797..8385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7877..7896)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8556..9347,9348..9416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9179..9198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9417..9521)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9589..9607
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgaattca ggcttggagg atacgtatga ctaaaaaaat ttcattcatt
      421 attaacggcc aggttgaaat ctttcccgaa ggtgatgatt tagtgcaatc cattaatttt
      481 ggtgataata gtgtttacct gccaatattg aatgactctc atgtaaaaaa cattattgat
      541 tgtaatggaa ataacgaatt acggttgcat aacattgtca attttctcta tacggtaggg
      601 caaagatgga aaaatgaaga atactcaaga cgcaggacat acattcgtga cttaaaaaaa
      661 tatatgggat attcagaaga aatggctaag ctagaggcca attggatatc tatgatttta
      721 tgttctaaag gcggccttta tgatgttgta gaaaatgaac ttggttctcg ccatatcatg
      781 gatgaatggc tacctcagga tgaaagttat gttcgggctt ttccgaaagg taaatctgta
      841 catctgttgg caggtaatgt tccattatct gggatcatgt ctatattacg cgcaatttta
      901 actaagaatc agtgtattat aaaaacatcg tcaaccgatc cttttaccgc taatgcatta
      961 gcgttaagtt ttattgatgt agaccctaat catccgataa cgcgctcttt atctgttata
     1021 tattggcccc accaaggtga tacatcactc gcaaaagaaa ttatgcaaca tgcggatgtt
     1081 attgtcgctt ggggagggcc agatgcgatt aattgggcgg tagagcatgc gccatcttat
     1141 gctgatgtga ttaaatttgg ttctaaaaag agtctttgca ttatcgataa tcctgttgat
     1201 ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt tttacgatca gcgagcttgt
     1261 ttttctgccc aaaacatata ttacatggga aatcattatg aggaatttaa gttagcgttg
     1321 atagaaaaac ttaatctata tgcgcatata ttaccgaatg ccaaaaaaga ttttgatgaa
     1381 aaggcggcct attctttagt tcaaaaagaa agcttgtttg ctggattaaa agtagaggtg
     1441 gatattcatc aacgttggat gattattgag tcaaatgcag gtgtggaatt taatcaacca
     1501 cttggcagat gtgtgtacct tcatcacgtc gataatattg agcaaatatt gccttatgtt
     1561 caaaaaaata agacgcaaac catatctatt tttccttggg agtcatcatt taaatatcga
     1621 gatgcgttag cattaaaagg tgcggaaagg attgtagaag caggaatgaa taacatattt
     1681 cgagttggtg gatctcatga cggaatgaga ccgttgcaac gattagtgac atatatttct
     1741 catgaaaggc catctaacta tacggctaag gatgttgcgg ttgaaataga acagactcga
     1801 ttcctggaag aagataagtt ccttgtattt gtcccataat aggtaaaaag tatggaaaat
     1861 gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg aaggaaataa aaaaattcat
     1921 gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa agaatgccat tattattgcg
     1981 tctggttttg cccgcaggat ggatcatttt gctggtctgg cggaatattt atcgcggaat
     2041 ggatttcatg tgatccgcta tgattcgctt caccacgttg gattgagttc agggacaatt
     2101 gatgaattta caatgtctat aggaaagcag agcttgttag cagtggttga ttggttaact
     2161 acacgaaaaa taaataactt cggtatgttg gcttcaagct tatctgcgcg gatagcttat
     2221 gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta
     2281 agatattctc ttgaaagagc tttagggttt gattatctca gtctacccat taatgaattg
     2341 ccgaataatc tagattttga aggccataaa ttgggtgctg aagtctttgc gagagattgt
     2401 cttgattttg gttgggaaga tttagcttct acaattaata acatgatgta tcttgatata
     2461 ccgtttattg cttttactgc aaataacgat aattgggtca agcaagatga agttatcaca
     2521 ttgttatcaa atattcgtag taatcgatgc aagatatatt ctttgttagg aagttcgcat
     2581 gacttgagtg aaaatttagt ggtcctgcgc aatttttatc aatcggttac gaaagccgct
     2641 atcgcgatgg ataatgatca tctggatatt gatgttgata ttactgaacc gtcatttgaa
     2701 catttaacta ttgcgacagt caatgaacgc cgaatgagaa ttgagattga aaatcaagca
     2761 atttctctgt cttaaaatct attgagatat tctatcactc aaatagcaat ataaggactc
     2821 tctatgaaat ttggaaactt tttgcttaca taccaacctc cccaattttc tcaaacagag
     2881 gtaatgaaac gtttggttaa attaggtcgc atctctgagg agtgtggttt tgataccgta
     2941 tggttactgg agcatcattt cacggagttt ggtttgcttg gtaaccctta tgtcgctgct
     3001 gcatatttac ttggcgcgac taaaaaattg aatgtaggaa ctgccgctat tgttcttccc
     3061 acagcccatc cagtacgcca acttgaagat gtgaatttat tggatcaaat gtcaaaagga
     3121 cgatttcggt ttggtatttg ccgagggctt tacaacaagg actttcgcgt attcggcaca
     3181 gatatgaata acagtcgcgc cttagcggaa tgctggtacg ggctgataaa gaatggcatg
     3241 acagagggat atatggaagc tgataatgaa catatcaagt tccataaggt aaaagtaaac
     3301 cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg
     3361 actgagtggg ctgctcaatt tggcctaccg atgatattaa gttggattat aaatactaac
     3421 gaaaagaaag cacaacttga gctttataat gaagtggctc aagaatatgg gcacgatatt
     3481 cataatatcg accattgctt atcatatata acatctgtag atcatgactc aattaaagcg
     3541 aaagagattt gccggaaatt tctggggcat tggtatgatt cttatgtgaa tgctacgact
     3601 atttttgatg attcagacca aacaagaggt tatgatttca ataaagggca gtggcgtgac
     3661 tttgtattaa aaggacataa agatactaat cgccgtattg attacagtta cgaaatcaat
     3721 cccgtgggaa cgccgcagga atgtattgac ataattcaaa aagacattga tgctacagga
     3781 atatcaaata tttgttgtgg atttgaagct aatggaacag tagacgaaat tattgcttcc
     3841 atgaagctct tccagtctga tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta
     3901 tattagctaa ggagaaagaa atgaaatttg gattgttctt ccttaacttc atcaattcaa
     3961 caactgttca agaacaaagt atagttcgca tgcaggaaat aacggagtat gttgataagt
     4021 tgaattttga acagatttta gtgtatgaaa atcatttttc agataatggt gttgtcggcg
     4081 ctcctctgac tgtttctggt tttctgctcg gtttaacaga gaaaattaaa attggttcat
     4141 taaatcacat cattacaact catcatcctg tccgcatagc ggaggaagct tgcttattgg
     4201 atcagttaag tgaagggaga tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa
     4261 tgcatttttt taatcgcccg gttgaatatc aacagcaact atttgaagag tgttatgaaa
     4321 tcattaacga tgctttaaca acaggctatt gtaatccaga taacgatttt tatagcttcc
     4381 ctaaaatatc tgtaaatccc catgcttata cgccaggcgg acctcggaaa tatgtaacag
     4441 caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg tattcctctc atctttaagt
     4501 gggatgattc taatgatgtt agatatgaat atgctgaaag atataaagcc gttgcggata
     4561 aatatgacgt tgacctatca gagatagacc atcagttaat gatattagtt aactataacg
     4621 aagatagtaa taaagctaaa caagagacgc gtgcatttat tagtgattat gttcttgaaa
     4681 tgcaccctaa tgaaaatttc gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg
     4741 gaaattatac ggagtgtata actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa
     4801 gtgtattgct gtcctttgaa ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata
     4861 ttgttgatga taatattaag aagtaccaca tggaatatac ctaatagatt tcgagttgca
     4921 gcgaggcggc aagtgaacga atccccagga gcatagataa ctatgtgact ggggtgagtg
     4981 aaagcagcca acaaagcagc agcttgaaag atgaagggta taaaagagta tgacagcagt
     5041 gctgccatac tttctaatat tatcttgagg agtaaaacag gtatgacttc atatgttgat
     5101 aaacaagaaa ttacagcaag ctcagaaatt gatgatttga ttttttcgag cgatccatta
     5161 gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt
     5221 aatcattata aacattgtcg agaatatcgt cactactgtc aggcacacaa agtagatgac
     5281 aatattacgg aaattgatga catacctgta ttcccaacat cggtttttaa gtttactcgc
     5341 ttattaactt ctcaggaaaa cgagattgaa agttggttta ccagtagcgg cacgaatggt
     5401 ttaaaaagtc aggtggcgcg tgacagatta agtattgaga gactcttagg ctctgtgagt
     5461 tatggcatga aatatgttgg tagttggttt gatcatcaaa tagaattagt caatttggga
     5521 ccagatagat ttaatgctca taatatttgg tttaaatatg ttatgagttt ggtggaattg
     5581 ttatatccta cgacatttac cgtaacagaa gaacgaatag attttgttaa aacattgaat
     5641 agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc ttattggttc gccatacttt
     5701 atttatttac tctgccatta tatgaaagat aaaaaaatct cattttctgg agataaaagc
     5761 ctttatatca taaccggagg cggctggaaa agttacgaaa aagaatctct gaaacgtgat
     5821 gatttcaatc atcttttatt tgatactttc aatctcagtg atattagtca gatccgagat
     5881 atatttaatc aagttgaact caacacttgt ttctttgagg atgaaatgca gcgtaaacat
     5941 gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat
     6001 ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa ccagttatcc agcatttatt
     6061 gttaccgatg atgtcgggat aattagcaga gaatatggta agtatcccgg cgtgctcgtt
     6121 gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt gtgctttaag cttaaccgaa
     6181 gcgtttgata gttgatgacc tgcaggcatg caagcttgcg gccgccaccg cggtggagct
     6241 cggtacccgg ggatcctcta gactctgcca gtgttacaac caattaacca attctgatta
     6301 cgggcagggc atgctcatgt agagcgcctg ttcgccatcg ctggccgtgc cgtcgtacag
     6361 ggcggtgtcc aggccgcaga gggtgaaccc catacgacga tacgcgtgga tcgccggtgc
     6421 gttgacgttg gtgacttcca gccagaggtg acctgcacca cgttcacggg caaattccgt
     6481 tgctaaaccc atcaatgcac gaccgacacc gtgaccacgg tgttctggtg cgacttcgat
     6541 gtcttcgacg gtcagacggc ggttccagcc gctatagctg acgaccacga agcctgccag
     6601 gtcgccatcg tcaccatacg cgacgaacgt ccgggagtcc ggatcaccgt cttcaccatc
     6661 gtccgattcg tcatcgcttt cgtcgtccgg aaacaccttg gtcaggggcg ggtcaaccgg
     6721 cacctcccgc agggtgaagc cgtcaccggt tgcggtgaca cggaagacgg tgtcggtggt
     6781 gaaggaccca tccagtgctt cgatggcttc ggcgtcaccc gggacagagg tgcgataccg
     6841 ataagccgtg tcatcaaggg tcgtacccat tactgtcctc cgtattactg tttatgtaag
     6901 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     6961 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga
     7021 tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca
     7081 actggtccac ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg
     7141 atggggcgat tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc
     7201 gctagcggag tgtatactgg cttctgcagg catgcaagct ttaaaagcct tatatattct
     7261 tttttttctt ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt
     7321 ttattgttca aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg
     7381 agagcttagt acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta
     7441 aacatgagag cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac
     7501 tgccaacgac tacgcactag ccaacaagag cttcagggtt gagatgtgta taagagacag
     7561 ctgtcttaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7621 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7681 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7741 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7801 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7861 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7921 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7981 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8041 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8101 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8161 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8221 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8281 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8401 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8461 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8521 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8581 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8641 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8701 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8761 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8821 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8881 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8941 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9001 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9061 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9121 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9181 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9241 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9301 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9361 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9421 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9481 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9541 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9601 acgaggccct ttcgtc
//
