LOCUS       sequence-232208-        9774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial luminescence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-proD/Lux-nat
FEATURES             Location/Qualifiers
     source          1..9774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             555..1997
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             2010..2933
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2982..4064
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4079..5062
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5241..6353
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     misc_feature    7700..7718
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(7784..7801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7955..8543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8035..8054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8714..9505,9506..9574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9337..9356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9575..9679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9747..9765
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgcgcgaa ttccacagct aacaccacgt cgtccctatc tgctgcccta
      421 ggtctatgag tggttgctgg ataactttac gggcatgcat aaggctcgta taatatattc
      481 agggagacca caacggtttc cctctacaaa taattttgtt taactttgaa ttcgcgcagg
      541 cttggaggat acgtatgact aaaaaaattt cattcattat taacggccag gttgaaatct
      601 ttcccgaagg tgatgattta gtgcaatcca ttaattttgg tgataatagt gtttacctgc
      661 caatattgaa tgactctcat gtaaaaaaca ttattgattg taatggaaat aacgaattac
      721 ggttgcataa cattgtcaat tttctctata cggtagggca aagatggaaa aatgaagaat
      781 actcaagacg caggacatac attcgtgact taaaaaaata tatgggatat tcagaagaaa
      841 tggctaagct agaggccaat tggatatcta tgattttatg ttctaaaggc ggcctttatg
      901 atgttgtaga aaatgaactt ggttctcgcc atatcatgga tgaatggcta cctcaggatg
      961 aaagttatgt tcgggctttt ccgaaaggta aatctgtaca tctgttggca ggtaatgttc
     1021 cattatctgg gatcatgtct atattacgcg caattttaac taagaatcag tgtattataa
     1081 aaacatcgtc aaccgatcct tttaccgcta atgcattagc gttaagtttt attgatgtag
     1141 accctaatca tccgataacg cgctctttat ctgttatata ttggccccac caaggtgata
     1201 catcactcgc aaaagaaatt atgcaacatg cggatgttat tgtcgcttgg ggagggccag
     1261 atgcgattaa ttgggcggta gagcatgcgc catcttatgc tgatgtgatt aaatttggtt
     1321 ctaaaaagag tctttgcatt atcgataatc ctgttgattt gacgtccgca gcgacaggtg
     1381 cggctcatga tgtttgtttt tacgatcagc gagcttgttt ttctgcccaa aacatatatt
     1441 acatgggaaa tcattatgag gaatttaagt tagcgttgat agaaaaactt aatctatatg
     1501 cgcatatatt accgaatgcc aaaaaagatt ttgatgaaaa ggcggcctat tctttagttc
     1561 aaaaagaaag cttgtttgct ggattaaaag tagaggtgga tattcatcaa cgttggatga
     1621 ttattgagtc aaatgcaggt gtggaattta atcaaccact tggcagatgt gtgtaccttc
     1681 atcacgtcga taatattgag caaatattgc cttatgttca aaaaaataag acgcaaacca
     1741 tatctatttt tccttgggag tcatcattta aatatcgaga tgcgttagca ttaaaaggtg
     1801 cggaaaggat tgtagaagca ggaatgaata acatatttcg agttggtgga tctcatgacg
     1861 gaatgagacc gttgcaacga ttagtgacat atatttctca tgaaaggcca tctaactata
     1921 cggctaagga tgttgcggtt gaaatagaac agactcgatt cctggaagaa gataagttcc
     1981 ttgtatttgt cccataatag gtaaaaagta tggaaaatga atcaaaatat aaaaccatcg
     2041 accacgttat ttgtgttgaa ggaaataaaa aaattcatgt ttgggaaacg ctgccagaag
     2101 aaaacagccc aaagagaaag aatgccatta ttattgcgtc tggttttgcc cgcaggatgg
     2161 atcattttgc tggtctggcg gaatatttat cgcggaatgg atttcatgtg atccgctatg
     2221 attcgcttca ccacgttgga ttgagttcag ggacaattga tgaatttaca atgtctatag
     2281 gaaagcagag cttgttagca gtggttgatt ggttaactac acgaaaaata aataacttcg
     2341 gtatgttggc ttcaagctta tctgcgcgga tagcttatgc aagcctatct gaaatcaatg
     2401 cttcgttttt aatcaccgca gtcggtgttg ttaacttaag atattctctt gaaagagctt
     2461 tagggtttga ttatctcagt ctacccatta atgaattgcc gaataatcta gattttgaag
     2521 gccataaatt gggtgctgaa gtctttgcga gagattgtct tgattttggt tgggaagatt
     2581 tagcttctac aattaataac atgatgtatc ttgatatacc gtttattgct tttactgcaa
     2641 ataacgataa ttgggtcaag caagatgaag ttatcacatt gttatcaaat attcgtagta
     2701 atcgatgcaa gatatattct ttgttaggaa gttcgcatga cttgagtgaa aatttagtgg
     2761 tcctgcgcaa tttttatcaa tcggttacga aagccgctat cgcgatggat aatgatcatc
     2821 tggatattga tgttgatatt actgaaccgt catttgaaca tttaactatt gcgacagtca
     2881 atgaacgccg aatgagaatt gagattgaaa atcaagcaat ttctctgtct taaaatctat
     2941 tgagatattc tatcactcaa atagcaatat aaggactctc tatgaaattt ggaaactttt
     3001 tgcttacata ccaacctccc caattttctc aaacagaggt aatgaaacgt ttggttaaat
     3061 taggtcgcat ctctgaggag tgtggttttg ataccgtatg gttactggag catcatttca
     3121 cggagtttgg tttgcttggt aacccttatg tcgctgctgc atatttactt ggcgcgacta
     3181 aaaaattgaa tgtaggaact gccgctattg ttcttcccac agcccatcca gtacgccaac
     3241 ttgaagatgt gaatttattg gatcaaatgt caaaaggacg atttcggttt ggtatttgcc
     3301 gagggcttta caacaaggac tttcgcgtat tcggcacaga tatgaataac agtcgcgcct
     3361 tagcggaatg ctggtacggg ctgataaaga atggcatgac agagggatat atggaagctg
     3421 ataatgaaca tatcaagttc cataaggtaa aagtaaaccc cgcggcgtat agcagaggtg
     3481 gcgcaccggt ttatgtggtg gctgaatcag cttcgacgac tgagtgggct gctcaatttg
     3541 gcctaccgat gatattaagt tggattataa atactaacga aaagaaagca caacttgagc
     3601 tttataatga agtggctcaa gaatatgggc acgatattca taatatcgac cattgcttat
     3661 catatataac atctgtagat catgactcaa ttaaagcgaa agagatttgc cggaaatttc
     3721 tggggcattg gtatgattct tatgtgaatg ctacgactat ttttgatgat tcagaccaaa
     3781 caagaggtta tgatttcaat aaagggcagt ggcgtgactt tgtattaaaa ggacataaag
     3841 atactaatcg ccgtattgat tacagttacg aaatcaatcc cgtgggaacg ccgcaggaat
     3901 gtattgacat aattcaaaaa gacattgatg ctacaggaat atcaaatatt tgttgtggat
     3961 ttgaagctaa tggaacagta gacgaaatta ttgcttccat gaagctcttc cagtctgatg
     4021 tcatgccatt tcttaaagaa aaacaacgtt cgctattata ttagctaagg agaaagaaat
     4081 gaaatttgga ttgttcttcc ttaacttcat caattcaaca actgttcaag aacaaagtat
     4141 agttcgcatg caggaaataa cggagtatgt tgataagttg aattttgaac agattttagt
     4201 gtatgaaaat catttttcag ataatggtgt tgtcggcgct cctctgactg tttctggttt
     4261 tctgctcggt ttaacagaga aaattaaaat tggttcatta aatcacatca ttacaactca
     4321 tcatcctgtc cgcatagcgg aggaagcttg cttattggat cagttaagtg aagggagatt
     4381 tattttaggg tttagtgatt gcgaaaaaaa agatgaaatg cattttttta atcgcccggt
     4441 tgaatatcaa cagcaactat ttgaagagtg ttatgaaatc attaacgatg ctttaacaac
     4501 aggctattgt aatccagata acgattttta tagcttccct aaaatatctg taaatcccca
     4561 tgcttatacg ccaggcggac ctcggaaata tgtaacagca accagtcatc atattgttga
     4621 gtgggcggcc aaaaaaggta ttcctctcat ctttaagtgg gatgattcta atgatgttag
     4681 atatgaatat gctgaaagat ataaagccgt tgcggataaa tatgacgttg acctatcaga
     4741 gatagaccat cagttaatga tattagttaa ctataacgaa gatagtaata aagctaaaca
     4801 agagacgcgt gcatttatta gtgattatgt tcttgaaatg caccctaatg aaaatttcga
     4861 aaataaactt gaagaaataa ttgcagaaaa cgctgtcgga aattatacgg agtgtataac
     4921 tgcggctaag ttggcaattg aaaagtgtgg tgcgaaaagt gtattgctgt cctttgaacc
     4981 aatgaatgat ttgatgagcc aaaaaaatgt aatcaatatt gttgatgata atattaagaa
     5041 gtaccacatg gaatatacct aatagatttc gagttgcagc gaggcggcaa gtgaacgaat
     5101 ccccaggagc atagataact atgtgactgg ggtgagtgaa agcagccaac aaagcagcag
     5161 cttgaaagat gaagggtata aaagagtatg acagcagtgc tgccatactt tctaatatta
     5221 tcttgaggag taaaacaggt atgacttcat atgttgataa acaagaaatt acagcaagct
     5281 cagaaattga tgatttgatt ttttcgagcg atccattagt gtggtcttac gacgagcagg
     5341 aaaaaatcag aaagaaactt gtgcttgatg catttcgtaa tcattataaa cattgtcgag
     5401 aatatcgtca ctactgtcag gcacacaaag tagatgacaa tattacggaa attgatgaca
     5461 tacctgtatt cccaacatcg gtttttaagt ttactcgctt attaacttct caggaaaacg
     5521 agattgaaag ttggtttacc agtagcggca cgaatggttt aaaaagtcag gtggcgcgtg
     5581 acagattaag tattgagaga ctcttaggct ctgtgagtta tggcatgaaa tatgttggta
     5641 gttggtttga tcatcaaata gaattagtca atttgggacc agatagattt aatgctcata
     5701 atatttggtt taaatatgtt atgagtttgg tggaattgtt atatcctacg acatttaccg
     5761 taacagaaga acgaatagat tttgttaaaa cattgaatag tcttgaacga ataaaaaatc
     5821 aagggaaaga tctttgtctt attggttcgc catactttat ttatttactc tgccattata
     5881 tgaaagataa aaaaatctca ttttctggag ataaaagcct ttatatcata accggaggcg
     5941 gctggaaaag ttacgaaaaa gaatctctga aacgtgatga tttcaatcat cttttatttg
     6001 atactttcaa tctcagtgat attagtcaga tccgagatat atttaatcaa gttgaactca
     6061 acacttgttt ctttgaggat gaaatgcagc gtaaacatgt tccgccgtgg gtatatgcgc
     6121 gagcgcttga tcctgaaacg ttgaaacctg tacctgatgg aacgccgggg ttgatgagtt
     6181 atatggatgc gtcagcaacc agttatccag catttattgt taccgatgat gtcgggataa
     6241 ttagcagaga atatggtaag tatcccggcg tgctcgttga aattttacgt cgcgtcaata
     6301 cgaggacgca gaaagggtgt gctttaagct taaccgaagc gtttgatagt tgatgacctg
     6361 caggcatgca agcttgcggc cgccaccgcg gtggagctcg gtacccgggg atcctctaga
     6421 ctctgccagt gttacaacca attaaccaat tctgattacg ggcagggcat gctcatgtag
     6481 agcgcctgtt cgccatcgct ggccgtgccg tcgtacaggg cggtgtccag gccgcagagg
     6541 gtgaacccca tacgacgata cgcgtggatc gccggtgcgt tgacgttggt gacttccagc
     6601 cagaggtgac ctgcaccacg ttcacgggca aattccgttg ctaaacccat caatgcacga
     6661 ccgacaccgt gaccacggtg ttctggtgcg acttcgatgt cttcgacggt cagacggcgg
     6721 ttccagccgc tatagctgac gaccacgaag cctgccaggt cgccatcgtc accatacgcg
     6781 acgaacgtcc gggagtccgg atcaccgtct tcaccatcgt ccgattcgtc atcgctttcg
     6841 tcgtccggaa acaccttggt caggggcggg tcaaccggca cctcccgcag ggtgaagccg
     6901 tcaccggttg cggtgacacg gaagacggtg tcggtggtga aggacccatc cagtgcttcg
     6961 atggcttcgg cgtcacccgg gacagaggtg cgataccgat aagccgtgtc atcaagggtc
     7021 gtacccatta ctgtcctccg tattactgtt tatgtaagca gacagtttta ttgttcatga
     7081 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg
     7141 ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc
     7201 agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc
     7261 tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta
     7321 tgagtcagca acaccttctt cacgaggcag acctcagcgc tagcggagtg tatactggct
     7381 tctgcaggca tgcaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     7441 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     7501 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     7561 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
     7621 aacatgagag cttagtacgt actatcaaca ggttgaactg ccaacgacta cgcactagcc
     7681 aacaagagct tcagggttga gatgtgtata agagacagct gtcttaatga atcggccaac
     7741 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9721 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
