LOCUS       sequence-232212-        4086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mClover promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-mClover-nat
FEATURES             Location/Qualifiers
     source          1..4086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    2012..2030
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(2096..2113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2267..2855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2347..2366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3026..3817,3818..3886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3649..3668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3887..3991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4059..4077
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattytct mgggkrtycy
      361 cgsrtayygn gctcgaattc taaggaggaa aaaaaatggt gagtaaaggc gaagaactgt
      421 ttaccggcgt agtaccaatt cttgttgaac ttgatggcga cgtgaatggt cacaaattct
      481 cggtccgcgg tgaaggcgag ggtgacgcaa ccaacggcaa gctgacgctg aaatttattt
      541 gcaccaccgg taaactgccg gttccgtggc ctacgctcgt gaccacgttt ggttatggtg
      601 tagcatgctt ttcccgttac ccggaccaca tgaagcagca tgactttttt aaatccgcta
      661 tgccggaagg ttatgttcag gaacgcacca tcagctttaa aaacgacggt acgtacaaaa
      721 cgcgcgccga agtgaaattt gaaggtgata ccctcgttaa ccgcattgag ctgaagggca
      781 ttgatttcaa agaagatggt aatatccttg gtcataaact ggaatacaat ttcaattcgc
      841 acaacgtgta tattacggcc gataagcaga aaaatggtat taaggcaaat ttcaaaatcc
      901 gtcataacgt ggaagacggt tccgtccagc ttgctgatca ttaccagcag aacacgccga
      961 ttggcgatgg tccggtactg ctgcctgata accactacct ttctcaccag tcagcacttt
     1021 ccaaagatcc gaacgaaaaa cgcgatcata tggtactgct cgagtttgta acggccgcgg
     1081 gcatcaccca cggcatggac gaactttaca aataagtcga cggaggacag taatgggtac
     1141 gacccttgat gacacggctt atcggtatcg cacctctgtc ccgggtgacg ccgaagccat
     1201 cgaagcactg gatgggtcct tcaccaccga caccgtcttc cgtgtcaccg caaccggtga
     1261 cggcttcacc ctgcgggagg tgccggttga cccgcccctg accaaggtgt ttccggacga
     1321 cgaaagcgat gacgaatcgg acgatggtga agacggtgat ccggactccc ggacgttcgt
     1381 cgcgtatggt gacgatggcg acctggcagg cttcgtggtc gtcagctata gcggctggaa
     1441 ccgccgtctg accgtcgaag acatcgaagt cgcaccagaa caccgtggtc acggtgtcgg
     1501 tcgtgcattg atgggtttag caacggaatt tgcccgtgaa cgtggtgcag gtcacctctg
     1561 gctggaagtc accaacgtca acgcaccggc gatccacgcg tatcgtcgta tggggttcac
     1621 cctctgcggc ctggacaccg ccctgtacga cggcacggcc agcgatggcg aacaggcgct
     1681 ctacatgagc atgccctgcc cgtaaaagct ttaaaagcct tatatattct tttttttctt
     1741 ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca
     1801 aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt
     1861 acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag
     1921 cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac tgccaacgac
     1981 tacgcactag ccaacaagag cttcagggtt gagatgtgta taagagacag ctgtcttaat
     2041 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2701 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4021 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4081 ttcgtc
//
