LOCUS       sequence-232213-       12425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry (without start and stop codons) in pGFL1010.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12425)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 12425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1259R
FEATURES             Location/Qualifiers
     source          1..12425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(445..456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2677..3381
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     3087..3106
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3383..3399)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3383..3399)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4312..4329)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4312..4328)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4321..4343)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4531..4555
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5856..6485
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6914..7987
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8053..8247
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8483..8505)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8591..8731
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8646..8665
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8746..8763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8917..9505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8997..9016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10930..11724)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12149..12173
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12251..12425
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgacaatc acgagcgagc gaacaaacag gcagagttac aaacgccatc actacgcctt
       61 gtcttttcgt ctctgttacg tgtcgccacc cccatgcgag ctacacgtct gcgcctgaag
      121 tcacaaggct tgcaatttca tgacgatgaa caacactgac acttctgtta cagagcacct
      181 cgaactaagc ccagcgccag cttcaatgct cgccgcgatc cccagcctct ggtcaaccct
      241 cgctcaggtg aggcagacga atcgtaagtt gcgccaacac cgctcctaca ccgccaagac
      301 ccataccgcc gtcgcatttg gacaagcaca agactgattt gaccgtggca tagattcatt
      361 ggcaagacgg gaggagagat tttccacgag atgatgctga ggcaaaacgt caagcatatc
      421 tgtaagcgtc taattctaca aattcctacc ctcgatttca accacatatt caaccacata
      481 ttctgacaca tgagtcacag tcggttaccc tggcggtgct atccttcccg tgttcgacgc
      541 gatctacaac tcgaagcaca tcgactttgt tctgcccaag catgagcaag gcgccggcca
      601 catggcagag ggctatgctc gcgcttcagg caaacccggc gttgttctcg tcacctccgg
      661 ccccggtgcc acaaatgtca tcactcccat ggccgacgct cttgccgacg gtacacctct
      721 ggttgtattc tcaggacagg ttgttacctc tgatattgga agcgacgcct tccaggaggc
      781 cgacgtcata ggcatctccc ggtcttgcac caagtggaac gtcatggtta agagcgctga
      841 cgagctcccg aggagaatta acgaggcctt tgagattgcc accagtgggc gacctgggcc
      901 tgtcttggtc gatctgccca aggacgtcac ggctagtgtg ctgaggaggg ctatccccac
      961 cgagacctcg attccctcta ttagcgcagc agcacgggct gtccaagagg caggccgaaa
     1021 gcagcttgag cactccatca aacgcgtagc cgatctcgtc aacattgcca agaagcccgt
     1081 catatatgcc ggccaaggtg tcattttgtc ggaaggcggc gttgaacttc tcaaggcgct
     1141 tgccgacaag gcctcgattc ctgtcaccac cactctgcat ggtctgggag cctttgacga
     1201 gctcgacgag aaggcactgc acatgcttgg tatgcacggt tcggcttatg ccaacatgtc
     1261 catgcaagag gccgatttga tcattgccct tggtggccgc ttcgatgacc gtgtcactgg
     1321 cagcatcccc aaatttgctc ctgccgccaa gctagctgct gctgaaggac gcggaggtat
     1381 tgtccacttc gagattatgc ccaagaacat caacaaggtc gtccaagcaa cagaggccat
     1441 tgagggcgac gttgcttcga acttgaagct gttgctcccc aagattgaac aacgatccat
     1501 gaccgatcgc aaggagtggt tcgaccagat caaggagtgg aaggagaagt ggcctctgtc
     1561 acattatgag agggccgagc gtagtggtct catcaagcct cagactctga tcgaggagct
     1621 gagcaacctg actgctgacc gcaaggacat gacctacatc acaaccggtg ttggccagca
     1681 ccaaatgtgg acagcacaac atttcaggtg gaggcaccca cggtccatga tcacctctgg
     1741 cggtttggga accatgggat atggtctgcc ggcagcgatt ggcgccaagg ttgctaggcc
     1801 agatgctttg gtcattgaca tcgacggcga cgcatcgttc aacatgactc tgacagagct
     1861 ttcgacggcg gcacagttca acattggcgt caaggtcatt gtcttgaaca acgaggagca
     1921 gggaatggtg acccaatggc agaacttgtt ctacgaggac cgctactcac atacacacca
     1981 gcgcaaccca gacttcatga agctcgccga tgcaatggac gttcaacatc gccgtgtttc
     2041 gaagcctgac gatgtcgttg atgctctgac gtggctgatc aacaccgacg gccccgctct
     2101 gcttgaggtg atgactgata agaaggttcc tgttctgccc atggtgcccg gaggtaacgg
     2161 cctgcacgag ttcatcacgt ttgatgccag taagtaatat atccaatgca ctcctgtggc
     2221 ccattttgag ccttgggtat gattctagtt caacttgcta accagtgcat caactcgtat
     2281 aggcaaggat aagcaacgga gagagctgat gcgcgcgagg acgaatggcc tgcacggtta
     2341 agttgataat gggaattgat tattgcacgg gaatttgcat ctcgactaaa tcattccact
     2401 caacattcag gctcctctgc gcacgtaaag tgccaaaggc aataccctgc tcggtggaat
     2461 gccgccgggc ttgtcgattt tacgcacata tgcgcattct tgacttgaag cggaggagtt
     2521 cttcgttgcg ggttacagtg ttttaataaa agaatggtca aatcaaactg ctagatatac
     2581 ctgtcagaca ctctagttgt tgacccctat actcttaata catcagacag tacatgcatg
     2641 ttgcatgatg atgctcgaga attcgagctc ggtaccgtga gcaagggcga ggaggataac
     2701 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2761 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2821 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2881 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2941 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggtggcgtg
     3001 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     3061 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     3121 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     3181 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     3241 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     3301 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     3361 ggcatggacg agctgtacaa gggatccact agttctagag tcgacctgca ggcatgctcc
     3421 acagggacaa ataccctcca ggggtaattc tattttggac aaaaagttgg aacttttgtg
     3481 ataccatggt gttaagaatg tcttatggac aaggggacga tacatttgag ctgcgggaat
     3541 ggttagcggc ttgtgtaaat attgcatcat tttttttttc tcttttactt ttttcttctt
     3601 cttctggctt ggtattcgtg cgtttaattc gtttcattct tctactttat tcgaagcaca
     3661 ttccttgcgt catcagtatc attgatacaa gtgatgtttc caactgaaca aatgttacaa
     3721 cattggtccg ttccagcctt tgttcatgag cagcaccggt atttgaacct ccaggttaga
     3781 gtagcctggg aaatgcaacg agcattctgc ctaatactag ctgccactcg aaggcattta
     3841 tagttttgct gaggtttcaa tgacgcccct ctgaagacaa agtagcacaa cggcattgac
     3901 gagactacta ctggtggttg aactgacttt tgcaccttgc tgctgctctt tggaagaggc
     3961 ttgaccatcc actgcggaag aggtgcttgt tgccttggga tttacttact tgtccgtcat
     4021 cttggcggac tgggccgagc aaccaaccag catcaacctt ggtcgcagaa gacattttga
     4081 atcttggcaa agtgtgctat ctattttggg cgtggaaatg gttcttgagt ttgttaggtg
     4141 ggccctggga gggatgcgcg gtcaactcct cacgactgca taaatctaat ggagatgaag
     4201 gtgtcgttgg agaagttgca gattatatcg gtgcatgaag catttgaaca gtcccaaatt
     4261 ataccacttt catcgataca attctgtcaa tgactggttg tgaagcttgg cactggccgt
     4321 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4381 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4441 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     4501 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     4561 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     4621 tggtccattt gtatgtccat gccaaccaca gggttcccct cgggatcaaa gtactttgat
     4681 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     4741 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     4801 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     4861 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     4921 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     4981 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     5041 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     5101 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     5161 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     5221 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     5281 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     5341 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     5401 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     5461 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     5521 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     5581 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     5641 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     5701 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     5761 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     5821 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     5881 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     5941 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     6001 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     6061 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     6121 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     6181 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     6241 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     6301 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     6361 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     6421 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     6481 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     6541 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     6601 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     6661 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     6721 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     6781 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     6841 ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc
     6901 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     6961 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     7021 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     7081 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     7141 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     7201 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     7261 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     7321 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     7381 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     7441 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     7501 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     7561 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     7621 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     7681 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     7741 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     7801 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     7861 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     7921 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
     7981 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
     8041 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
     8101 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
     8161 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
     8221 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
     8281 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
     8341 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
     8401 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
     8461 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     8521 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     8581 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     8641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     8701 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     8761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     9001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     9061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     9121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     9181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     9241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     9301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     9361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     9421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     9481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9541 tcagtggaac gaaaactcac gttaagggct gatgaatccc ctaatgattt tggtaaaaat
     9601 cattaagtta aggtggatac acatcttgtc atatgatcaa atggtttcgc gaaaaatcaa
     9661 taatcagaca acaagatgtg cgaactcgat attttacacg actctcttta ccaattctgc
     9721 cccgaattac acttaaaacg actcaacagc ttaacgttgg cttgccacgc cttacttgac
     9781 tgtaaaactc tcactcttac cgaacttggc cgtaacctgc caaccaaagc gagaacaaaa
     9841 cataacatca aacgaatcga ccgattgtta ggtaatcgtc acctccacaa agagcgactc
     9901 gctgtatacc gttggcatgc tagctttatc tgttcgggca atacgatgcc cattgtactt
     9961 gttgactggt ctgatattcg tgagcaaaaa cgacttatgg tattgcgagc ttcagtcgca
    10021 ctacacggtc gttctgttac tctttatgag aaagcgttcc cgctttcaga gcaatgttca
    10081 aagaaagctc atgaccaatt tctagccgac cttgcgagca ttctaccgag taacaccaca
    10141 ccgctcattg tcagtgatgc tggctttaaa gtgccatggt ataaatccgt tgagaagctg
    10201 ggttggtact ggttaagtcg agtaagagga aaagtacaat atgcagacct aggagcggaa
    10261 aactggaaac ctatcagcaa cttacatgat atgtcatcta gtcactcaaa gactttaggc
    10321 tataagaggc tgactaaaag caatccaatc tcatgccaaa ttctattgta taaatctcgc
    10381 tctaaaggcc gaaaaaatca gcgctcgaca cggactcatt gtcaccaccc gtcacctaaa
    10441 atctactcag cgtcggcaaa ggagccatgg gttctagcaa ctaacttacc tgttgaaatt
    10501 cgaacaccca aacaacttgt taatatctat tcgaagcgaa tgcagattga agaaaccttc
    10561 cgagacttga aaagtcctgc ctacggacta ggcctacgcc atagccgaac gagcagctca
    10621 gagcgttttg atatcatgct gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg
    10681 ggcgttcatg ctcagaaaca aggttgggac aagcacttcc aggctaacac agtcagaaat
    10741 cgaaacgtac tctcaacagt tcgcttaggc atggaagttt tgcggcattc tggctacaca
    10801 ataacaaggg aagacttact cgtggctgca accctactag ctcaaaattt attcacacat
    10861 ggttacgctt tggggaaatt atgaggggat ctctcagcgt taagggattt tggtcatgca
    10921 ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    10981 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    11041 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    11101 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    11161 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    11221 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    11281 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    11341 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    11401 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    11461 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    11521 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    11581 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    11641 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    11701 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    11761 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    11821 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    11881 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
    11941 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    12001 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
    12061 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
    12121 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
    12181 cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat
    12241 taattcgggg gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
    12301 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
    12361 agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg
    12421 agaaa
//
