LOCUS       sequence-232216-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry-tagged human importin-alpha in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K
  TITLE     A rapid and simple method of evaluating the dimeric tendency of 
            fluorescent proteins in living cells using a truncated protein of 
            importin alpha as fusion tag.
  JOURNAL   Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 
            10.1271/bbb.110677. Epub 2012 Feb 7.
  PUBMED    22313767
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2012"; volume: "76(2):388-90. doi"; 
            pages: " 10.1271/bbb.110677. Epub 2012 Feb 7"
COMMENT     Sequence Label: pmCherry-imp alpha
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14..724
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(159..177)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     427..446
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagccgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
       61 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      241 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      361 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      421 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      481 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      541 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      601 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      661 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      721 gtaaagatct
//
