LOCUS       sequence-232222-       12434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP (without start and stop codons)  in pFGL1010.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 12434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1259
FEATURES             Location/Qualifiers
     source          1..12434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(445..456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(2677..2679,2680..3390)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2677..2679,2680..3390)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2719..2740)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2980..2999)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3327..3348
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3392..3408)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3392..3408)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4321..4338)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4321..4337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4330..4352)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4540..4564
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5865..6494
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6923..7996
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8062..8256
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8492..8514)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8600..8740
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8655..8674
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8755..8772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8926..9514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9006..9025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10939..11733)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12158..12182
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12260..12434
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgacaatc acgagcgagc gaacaaacag gcagagttac aaacgccatc actacgcctt
       61 gtcttttcgt ctctgttacg tgtcgccacc cccatgcgag ctacacgtct gcgcctgaag
      121 tcacaaggct tgcaatttca tgacgatgaa caacactgac acttctgtta cagagcacct
      181 cgaactaagc ccagcgccag cttcaatgct cgccgcgatc cccagcctct ggtcaaccct
      241 cgctcaggtg aggcagacga atcgtaagtt gcgccaacac cgctcctaca ccgccaagac
      301 ccataccgcc gtcgcatttg gacaagcaca agactgattt gaccgtggca tagattcatt
      361 ggcaagacgg gaggagagat tttccacgag atgatgctga ggcaaaacgt caagcatatc
      421 tgtaagcgtc taattctaca aattcctacc ctcgatttca accacatatt caaccacata
      481 ttctgacaca tgagtcacag tcggttaccc tggcggtgct atccttcccg tgttcgacgc
      541 gatctacaac tcgaagcaca tcgactttgt tctgcccaag catgagcaag gcgccggcca
      601 catggcagag ggctatgctc gcgcttcagg caaacccggc gttgttctcg tcacctccgg
      661 ccccggtgcc acaaatgtca tcactcccat ggccgacgct cttgccgacg gtacacctct
      721 ggttgtattc tcaggacagg ttgttacctc tgatattgga agcgacgcct tccaggaggc
      781 cgacgtcata ggcatctccc ggtcttgcac caagtggaac gtcatggtta agagcgctga
      841 cgagctcccg aggagaatta acgaggcctt tgagattgcc accagtgggc gacctgggcc
      901 tgtcttggtc gatctgccca aggacgtcac ggctagtgtg ctgaggaggg ctatccccac
      961 cgagacctcg attccctcta ttagcgcagc agcacgggct gtccaagagg caggccgaaa
     1021 gcagcttgag cactccatca aacgcgtagc cgatctcgtc aacattgcca agaagcccgt
     1081 catatatgcc ggccaaggtg tcattttgtc ggaaggcggc gttgaacttc tcaaggcgct
     1141 tgccgacaag gcctcgattc ctgtcaccac cactctgcat ggtctgggag cctttgacga
     1201 gctcgacgag aaggcactgc acatgcttgg tatgcacggt tcggcttatg ccaacatgtc
     1261 catgcaagag gccgatttga tcattgccct tggtggccgc ttcgatgacc gtgtcactgg
     1321 cagcatcccc aaatttgctc ctgccgccaa gctagctgct gctgaaggac gcggaggtat
     1381 tgtccacttc gagattatgc ccaagaacat caacaaggtc gtccaagcaa cagaggccat
     1441 tgagggcgac gttgcttcga acttgaagct gttgctcccc aagattgaac aacgatccat
     1501 gaccgatcgc aaggagtggt tcgaccagat caaggagtgg aaggagaagt ggcctctgtc
     1561 acattatgag agggccgagc gtagtggtct catcaagcct cagactctga tcgaggagct
     1621 gagcaacctg actgctgacc gcaaggacat gacctacatc acaaccggtg ttggccagca
     1681 ccaaatgtgg acagcacaac atttcaggtg gaggcaccca cggtccatga tcacctctgg
     1741 cggtttggga accatgggat atggtctgcc ggcagcgatt ggcgccaagg ttgctaggcc
     1801 agatgctttg gtcattgaca tcgacggcga cgcatcgttc aacatgactc tgacagagct
     1861 ttcgacggcg gcacagttca acattggcgt caaggtcatt gtcttgaaca acgaggagca
     1921 gggaatggtg acccaatggc agaacttgtt ctacgaggac cgctactcac atacacacca
     1981 gcgcaaccca gacttcatga agctcgccga tgcaatggac gttcaacatc gccgtgtttc
     2041 gaagcctgac gatgtcgttg atgctctgac gtggctgatc aacaccgacg gccccgctct
     2101 gcttgaggtg atgactgata agaaggttcc tgttctgccc atggtgcccg gaggtaacgg
     2161 cctgcacgag ttcatcacgt ttgatgccag taagtaatat atccaatgca ctcctgtggc
     2221 ccattttgag ccttgggtat gattctagtt caacttgcta accagtgcat caactcgtat
     2281 aggcaaggat aagcaacgga gagagctgat gcgcgcgagg acgaatggcc tgcacggtta
     2341 agttgataat gggaattgat tattgcacgg gaatttgcat ctcgactaaa tcattccact
     2401 caacattcag gctcctctgc gcacgtaaag tgccaaaggc aataccctgc tcggtggaat
     2461 gccgccgggc ttgtcgattt tacgcacata tgcgcattct tgacttgaag cggaggagtt
     2521 cttcgttgcg ggttacagtg ttttaataaa agaatggtca aatcaaactg ctagatatac
     2581 ctgtcagaca ctctagttgt tgacccctat actcttaata catcagacag tacatgcatg
     2641 ttgcatgatg atgctcgaga attcgagctc ggtaccgtga gcaagggcga ggagctgttc
     2701 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2761 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2821 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     2881 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2941 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3001 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3061 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     3121 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     3181 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     3241 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     3301 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     3361 atcactctcg gcatggacga gctgtacaag ggatccacta gttctagagt cgacctgcag
     3421 gcatgctcca cagggacaaa taccctccag gggtaattct attttggaca aaaagttgga
     3481 acttttgtga taccatggtg ttaagaatgt cttatggaca aggggacgat acatttgagc
     3541 tgcgggaatg gttagcggct tgtgtaaata ttgcatcatt ttttttttct cttttacttt
     3601 tttcttcttc ttctggcttg gtattcgtgc gtttaattcg tttcattctt ctactttatt
     3661 cgaagcacat tccttgcgtc atcagtatca ttgatacaag tgatgtttcc aactgaacaa
     3721 atgttacaac attggtccgt tccagccttt gttcatgagc agcaccggta tttgaacctc
     3781 caggttagag tagcctggga aatgcaacga gcattctgcc taatactagc tgccactcga
     3841 aggcatttat agttttgctg aggtttcaat gacgcccctc tgaagacaaa gtagcacaac
     3901 ggcattgacg agactactac tggtggttga actgactttt gcaccttgct gctgctcttt
     3961 ggaagaggct tgaccatcca ctgcggaaga ggtgcttgtt gccttgggat ttacttactt
     4021 gtccgtcatc ttggcggact gggccgagca accaaccagc atcaaccttg gtcgcagaag
     4081 acattttgaa tcttggcaaa gtgtgctatc tattttgggc gtggaaatgg ttcttgagtt
     4141 tgttaggtgg gccctgggag ggatgcgcgg tcaactcctc acgactgcat aaatctaatg
     4201 gagatgaagg tgtcgttgga gaagttgcag attatatcgg tgcatgaagc atttgaacag
     4261 tcccaaatta taccactttc atcgatacaa ttctgtcaat gactggttgt gaagcttggc
     4321 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4381 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4441 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
     4501 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
     4561 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     4621 tttatccgtt ggtccatttg tatgtccatg ccaaccacag ggttcccctc gggatcaaag
     4681 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     4741 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     4801 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     4861 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     4921 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     4981 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     5041 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     5101 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     5161 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     5221 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     5281 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     5341 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     5401 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     5461 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     5521 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     5581 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     5641 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     5701 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     5761 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     5821 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     5881 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     5941 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     6001 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     6061 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     6121 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     6181 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     6241 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     6301 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     6361 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     6421 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     6481 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     6541 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     6601 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     6661 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     6721 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     6781 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     6841 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     6901 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     6961 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     7021 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     7081 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     7141 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     7201 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     7261 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     7321 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     7381 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     7441 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     7501 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     7561 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     7621 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     7681 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     7741 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     7801 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     7861 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     7921 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     7981 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     8041 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     8101 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     8161 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     8221 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     8281 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     8341 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     8401 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     8461 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8521 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8581 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     8641 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     8701 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     8761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     9001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     9061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     9121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     9181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     9241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9361 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9541 gtctgacgct cagtggaacg aaaactcacg ttaagggctg atgaatcccc taatgatttt
     9601 ggtaaaaatc attaagttaa ggtggataca catcttgtca tatgatcaaa tggtttcgcg
     9661 aaaaatcaat aatcagacaa caagatgtgc gaactcgata ttttacacga ctctctttac
     9721 caattctgcc ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgcc
     9781 ttacttgact gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg
     9841 agaacaaaac ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa
     9901 gagcgactcg ctgtataccg ttggcatgct agctttatct gttcgggcaa tacgatgccc
     9961 attgtacttg ttgactggtc tgatattcgt gagcaaaaac gacttatggt attgcgagct
    10021 tcagtcgcac tacacggtcg ttctgttact ctttatgaga aagcgttccc gctttcagag
    10081 caatgttcaa agaaagctca tgaccaattt ctagccgacc ttgcgagcat tctaccgagt
    10141 aacaccacac cgctcattgt cagtgatgct ggctttaaag tgccatggta taaatccgtt
    10201 gagaagctgg gttggtactg gttaagtcga gtaagaggaa aagtacaata tgcagaccta
    10261 ggagcggaaa actggaaacc tatcagcaac ttacatgata tgtcatctag tcactcaaag
    10321 actttaggct ataagaggct gactaaaagc aatccaatct catgccaaat tctattgtat
    10381 aaatctcgct ctaaaggccg aaaaaatcag cgctcgacac ggactcattg tcaccacccg
    10441 tcacctaaaa tctactcagc gtcggcaaag gagccatggg ttctagcaac taacttacct
    10501 gttgaaattc gaacacccaa acaacttgtt aatatctatt cgaagcgaat gcagattgaa
    10561 gaaaccttcc gagacttgaa aagtcctgcc tacggactag gcctacgcca tagccgaacg
    10621 agcagctcag agcgttttga tatcatgctg ctaatcgccc tgatgcttca actaacatgt
    10681 tggcttgcgg gcgttcatgc tcagaaacaa ggttgggaca agcacttcca ggctaacaca
    10741 gtcagaaatc gaaacgtact ctcaacagtt cgcttaggca tggaagtttt gcggcattct
    10801 ggctacacaa taacaaggga agacttactc gtggctgcaa ccctactagc tcaaaattta
    10861 ttcacacatg gttacgcttt ggggaaatta tgaggggatc tctcagcgtt aagggatttt
    10921 ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    10981 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    11041 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    11101 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    11161 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    11221 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    11281 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    11341 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    11401 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    11461 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    11521 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    11581 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    11641 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    11701 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    11761 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    11821 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    11881 tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc
    11941 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
    12001 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
    12061 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
    12121 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    12181 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
    12241 acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga
    12301 aattttattg atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct
    12361 caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca
    12421 ttattatgga gaaa
//
