LOCUS       sequence-232227-       11450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introducing ectopic integration at the defined fungal ILV2-locus, 
            based on SRR (Sulfonylurea Resistance Reconstitution)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Naqvi Lab
  TITLE     ATMT vectors for targeted integration at ILV2-locus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFGL1009
FEATURES             Location/Qualifiers
     source          1..11450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(445..456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2408..2424)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2408..2424)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3337..3354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3337..3353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3346..3368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3556..3580
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4881..5510
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5939..7012
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7078..7272
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7508..7530)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7616..7756
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7671..7690
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7771..7788)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7942..8530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8022..8041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9955..10749)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11174..11198
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11276..11450
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgacaatc acgagcgagc gaacaaacag gcagagttac aaacgccatc actacgcctt
       61 gtcttttcgt ctctgttacg tgtcgccacc cccatgcgag ctacacgtct gcgcctgaag
      121 tcacaaggct tgcaatttca tgacgatgaa caacactgac acttctgtta cagagcacct
      181 cgaactaagc ccagcgccag cttcaatgct cgccgcgatc cccagcctct ggtcaaccct
      241 cgctcaggtg aggcagacga atcgtaagtt gcgccaacac cgctcctaca ccgccaagac
      301 ccataccgcc gtcgcatttg gacaagcaca agactgattt gaccgtggca tagattcatt
      361 ggcaagacgg gaggagagat tttccacgag atgatgctga ggcaaaacgt caagcatatc
      421 tgtaagcgtc taattctaca aattcctacc ctcgatttca accacatatt caaccacata
      481 ttctgacaca tgagtcacag tcggttaccc tggcggtgct atccttcccg tgttcgacgc
      541 gatctacaac tcgaagcaca tcgactttgt tctgcccaag catgagcaag gcgccggcca
      601 catggcagag ggctatgctc gcgcttcagg caaacccggc gttgttctcg tcacctccgg
      661 ccccggtgcc acaaatgtca tcactcccat ggccgacgct cttgccgacg gtacacctct
      721 ggttgtattc tcaggacagg ttgttacctc tgatattgga agcgacgcct tccaggaggc
      781 cgacgtcata ggcatctccc ggtcttgcac caagtggaac gtcatggtta agagcgctga
      841 cgagctcccg aggagaatta acgaggcctt tgagattgcc accagtgggc gacctgggcc
      901 tgtcttggtc gatctgccca aggacgtcac ggctagtgtg ctgaggaggg ctatccccac
      961 cgagacctcg attccctcta ttagcgcagc agcacgggct gtccaagagg caggccgaaa
     1021 gcagcttgag cactccatca aacgcgtagc cgatctcgtc aacattgcca agaagcccgt
     1081 catatatgcc ggccaaggtg tcattttgtc ggaaggcggc gttgaacttc tcaaggcgct
     1141 tgccgacaag gcctcgattc ctgtcaccac cactctgcat ggtctgggag cctttgacga
     1201 gctcgacgag aaggcactgc acatgcttgg tatgcacggt tcggcttatg ccaacatgtc
     1261 catgcaagag gccgatttga tcattgccct tggtggccgc ttcgatgacc gtgtcactgg
     1321 cagcatcccc aaatttgctc ctgccgccaa gctagctgct gctgaaggac gcggaggtat
     1381 tgtccacttc gagattatgc ccaagaacat caacaaggtc gtccaagcaa cagaggccat
     1441 tgagggcgac gttgcttcga acttgaagct gttgctcccc aagattgaac aacgatccat
     1501 gaccgatcgc aaggagtggt tcgaccagat caaggagtgg aaggagaagt ggcctctgtc
     1561 acattatgag agggccgagc gtagtggtct catcaagcct cagactctga tcgaggagct
     1621 gagcaacctg actgctgacc gcaaggacat gacctacatc acaaccggtg ttggccagca
     1681 ccaaatgtgg acagcacaac atttcaggtg gaggcaccca cggtccatga tcacctctgg
     1741 cggtttggga accatgggat atggtctgcc ggcagcgatt ggcgccaagg ttgctaggcc
     1801 agatgctttg gtcattgaca tcgacggcga cgcatcgttc aacatgactc tgacagagct
     1861 ttcgacggcg gcacagttca acattggcgt caaggtcatt gtcttgaaca acgaggagca
     1921 gggaatggtg acccaatggc agaacttgtt ctacgaggac cgctactcac atacacacca
     1981 gcgcaaccca gacttcatga agctcgccga tgcaatggac gttcaacatc gccgtgtttc
     2041 gaagcctgac gatgtcgttg atgctctgac gtggctgatc aacaccgacg gccccgctct
     2101 gcttgaggtg atgactgata agaaggttcc tgttctgccc atggtgcccg gaggtaacgg
     2161 cctgcacgag ttcatcacgt ttgatgccag taagtaatat atccaatgca ctcctgtggc
     2221 ccattttgag ccttgggtat gattctagtt caacttgcta accagtgcat caactcgtat
     2281 aggcaaggat aagcaacgga gagagctgat gcgcgcgagg acgaatggcc tgcacggtta
     2341 agttgataat gggaattgat tattgcacgg gaatttgcat ctcgagaatt cgagctcggt
     2401 acccggggat ccactagttc tagagtcgac ctgcaggcat gctccacagg gacaaatacc
     2461 ctccaggggt aattctattt tggacaaaaa gttggaactt ttgtgatacc atggtgttaa
     2521 gaatgtctta tggacaaggg gacgatacat ttgagctgcg ggaatggtta gcggcttgtg
     2581 taaatattgc atcatttttt ttttctcttt tacttttttc ttcttcttct ggcttggtat
     2641 tcgtgcgttt aattcgtttc attcttctac tttattcgaa gcacattcct tgcgtcatca
     2701 gtatcattga tacaagtgat gtttccaact gaacaaatgt tacaacattg gtccgttcca
     2761 gcctttgttc atgagcagca ccggtatttg aacctccagg ttagagtagc ctgggaaatg
     2821 caacgagcat tctgcctaat actagctgcc actcgaaggc atttatagtt ttgctgaggt
     2881 ttcaatgacg cccctctgaa gacaaagtag cacaacggca ttgacgagac tactactggt
     2941 ggttgaactg acttttgcac cttgctgctg ctctttggaa gaggcttgac catccactgc
     3001 ggaagaggtg cttgttgcct tgggatttac ttacttgtcc gtcatcttgg cggactgggc
     3061 cgagcaacca accagcatca accttggtcg cagaagacat tttgaatctt ggcaaagtgt
     3121 gctatctatt ttgggcgtgg aaatggttct tgagtttgtt aggtgggccc tgggagggat
     3181 gcgcggtcaa ctcctcacga ctgcataaat ctaatggaga tgaaggtgtc gttggagaag
     3241 ttgcagatta tatcggtgca tgaagcattt gaacagtccc aaattatacc actttcatcg
     3301 atacaattct gtcaatgact ggttgtgaag cttggcactg gccgtcgttt tacaacgtcg
     3361 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3421 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3481 gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt
     3541 taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta
     3601 ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttggtc catttgtatg
     3661 tccatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     3721 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     3781 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     3841 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     3901 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     3961 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     4021 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     4081 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     4141 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     4201 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     4261 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     4321 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     4381 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     4441 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     4501 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     4561 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     4621 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     4681 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     4741 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     4801 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     4861 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     4921 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     4981 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     5041 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     5101 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     5161 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     5221 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     5281 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     5341 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     5401 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     5461 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     5521 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     5581 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     5641 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     5701 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     5761 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     5821 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     5881 gctgggttgc ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     5941 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt
     6001 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc
     6061 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc
     6121 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt
     6181 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt
     6241 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg
     6301 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt
     6361 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga
     6421 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc
     6481 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca
     6541 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg
     6601 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat
     6661 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt
     6721 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg
     6781 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga
     6841 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg
     6901 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct
     6961 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga
     7021 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt
     7081 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg
     7141 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa
     7201 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct
     7261 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct
     7321 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg
     7381 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc
     7441 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg
     7501 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     7561 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     7621 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     7681 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7741 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7801 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7861 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7921 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7981 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8041 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8101 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8161 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8221 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8281 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8341 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8401 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8461 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8521 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8581 ctcacgttaa gggctgatga atcccctaat gattttggta aaaatcatta agttaaggtg
     8641 gatacacatc ttgtcatatg atcaaatggt ttcgcgaaaa atcaataatc agacaacaag
     8701 atgtgcgaac tcgatatttt acacgactct ctttaccaat tctgccccga attacactta
     8761 aaacgactca acagcttaac gttggcttgc cacgccttac ttgactgtaa aactctcact
     8821 cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa caaaacataa catcaaacga
     8881 atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt ataccgttgg
     8941 catgctagct ttatctgttc gggcaatacg atgcccattg tacttgttga ctggtctgat
     9001 attcgtgagc aaaaacgact tatggtattg cgagcttcag tcgcactaca cggtcgttct
     9061 gttactcttt atgagaaagc gttcccgctt tcagagcaat gttcaaagaa agctcatgac
     9121 caatttctag ccgaccttgc gagcattcta ccgagtaaca ccacaccgct cattgtcagt
     9181 gatgctggct ttaaagtgcc atggtataaa tccgttgaga agctgggttg gtactggtta
     9241 agtcgagtaa gaggaaaagt acaatatgca gacctaggag cggaaaactg gaaacctatc
     9301 agcaacttac atgatatgtc atctagtcac tcaaagactt taggctataa gaggctgact
     9361 aaaagcaatc caatctcatg ccaaattcta ttgtataaat ctcgctctaa aggccgaaaa
     9421 aatcagcgct cgacacggac tcattgtcac cacccgtcac ctaaaatcta ctcagcgtcg
     9481 gcaaaggagc catgggttct agcaactaac ttacctgttg aaattcgaac acccaaacaa
     9541 cttgttaata tctattcgaa gcgaatgcag attgaagaaa ccttccgaga cttgaaaagt
     9601 cctgcctacg gactaggcct acgccatagc cgaacgagca gctcagagcg ttttgatatc
     9661 atgctgctaa tcgccctgat gcttcaacta acatgttggc ttgcgggcgt tcatgctcag
     9721 aaacaaggtt gggacaagca cttccaggct aacacagtca gaaatcgaaa cgtactctca
     9781 acagttcgct taggcatgga agttttgcgg cattctggct acacaataac aagggaagac
     9841 ttactcgtgg ctgcaaccct actagctcaa aatttattca cacatggtta cgctttgggg
     9901 aaattatgag gggatctctc agcgttaagg gattttggtc atgcattcta ggtactaaaa
     9961 caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa
    10021 gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca
    10081 gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact
    10141 tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag
    10201 ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg
    10261 agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc
    10321 caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga
    10381 gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc
    10441 ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc
    10501 atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat
    10561 catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt
    10621 taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc
    10681 cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat
    10741 tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct
    10801 aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag
    10861 aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg
    10921 attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
    10981 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
    11041 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
    11101 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
    11161 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
    11221 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct
    11281 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
    11341 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
    11401 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
//
