LOCUS       sequence-232229-        9991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CenpC:mCherry (centromere marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 9991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1169R
FEATURES             Location/Qualifiers
     source          1..9991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             568..1275
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     978..997
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1277..1293)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1277..1293)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             1674..2225
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(3216..3233)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3216..3232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3225..3247)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3435..3459
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4760..5389
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5818..6891
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6957..7151
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7387..7409)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7495..7635
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7550..7569
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7650..7667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7821..8409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7901..7920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8496..9290)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9715..9739
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9817..9991
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt cgaggacgaa gaaccttcac cctgggaaca ggatcctggc gagctgatcg
       61 tgccagtcca gcagtggcag cctgagcacg aatttgaccc acccgggcct gatgacgagc
      121 tgcccgaggg cgaggacgta gtagcggtat caggaccagc gattaggact cgcgagatcc
      181 ttgggtcgag cttccggttc accaagacgc taagcttacc cttctttgga actggagtgg
      241 tggatctacc gccaggggct gagaaaaagg ccaagaactc acgcaagatg cacatgacct
      301 ttatagtgca cacggggcgg gtcactgtga cggtcaacaa cacggccttt tcgattggca
      361 agggtggcat gtggtttgtg cccagaggta tgtgtgcggt ccttttcatg tgttatgcgc
      421 ctcaaatact gatatatttc tacaggcaac tattacagca ttgcgaatga gggcgccaag
      481 tcgtccagga tattcttcgc gcaggcttgt gaatatagaa gacctgatga agatgagcag
      541 gacgaaatga ctgaaagcag cggtaccgtg agcaagggcg aggaggataa catggccatc
      601 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      661 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      721 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      781 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      841 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      901 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      961 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     1021 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     1081 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     1141 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     1201 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1261 gagctgtaca agtaaggatc cactagttct agagtcgagg atattgaagg agcacttttt
     1321 gggcttggct ggagctagtg gaggtcaaca atgaatgcct attttggttt agtcgtccag
     1381 gcggtgagca caaaatttgt gtcgtttgac aagatggttc atttaggcaa ctggtcagat
     1441 cagccccact tgtagcagta gcggcggcgc tcgaagtgtg actcttatta gcagacagga
     1501 acgaggacat tattatcatc tgctgcttgg tgcacgataa cttggtgcgt ttgtcaagca
     1561 aggtaagtga acgacccggt cataccttct taagttcgcc cttcctccct ttatttcaga
     1621 ttcaatctga cttacctatt ctacccaagc aacgcttcga ttaggaagta accatgagcc
     1681 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1741 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     1801 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     1861 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggtcc ctggaaggca cgcaacgcct
     1921 acgactggac ggccgaatcg accgtgtacg tctccccccg ccaccagcgg acgggactgg
     1981 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2041 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2101 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2161 tctggcagct ggacttcagc ctgccggttc cgccccgtcc ggtcctgccc gtcaccgaga
     2221 tttaggtcga cctgcagtaa ttcatcgcgg tgggttggtc tggttgacgg ataaaaaagt
     2281 atgataccaa tgttgacgat ctgttttacg cccaattctt gtttacctag ctcattgtct
     2341 gtatacccat ttgaaccctg cctaacacgt attctttcta atctttgggt cgtattgctt
     2401 gaggatcctg tgcatgtgat gtgattgcgt tgctttgaat taaatacaac gggctcgacc
     2461 caggtacgac cctctttacc tgacttttca cctgtccttc tatctacctt ccatttttac
     2521 ttatattcgc gtctaccttc tcagctaaaa ccagttgaaa cccccggaat aatattaatc
     2581 aaacctaagc cgaaacggca ttatggcatt gtattcaggc tgttcccgcc gcgatttaga
     2641 gacttagaag gactgctaga ctcccagcta tgtgaatgac ttcagttaaa accagcagaa
     2701 actcccgaaa cgatattgat caaaccccaa gccaaaatcc cattccatgc gttatttcca
     2761 gcccgtttcc accgcgattt catcaaagca gccgagaaat actcttgcca tcgataacag
     2821 cgatcagatt ctagctcttc gtcagggccg aatatccacc ctggcagcct cgctgccaac
     2881 catgccgtct cggagctgtt gctgcaactg cgccgtatta gcacatccga ctcagcttcc
     2941 tcccaaccga caatttgcct tttggtatct ggactgtcgg catcctcggt cttaattttt
     3001 ttatcaggca ttgcggctga ttgatggcga atatcccgaa atatgtagcc aactgcctat
     3061 tgggtgttat gttggaacta ctttgaaaat gtgctattag acgcgatgtt agtgaatcaa
     3121 gtgcggcgag gaagaatagg ggtggatcgg gcgtattgag gttcgacgga cccaaatcgt
     3181 ctgcgattat aatgagggag ggccagaagc ttggcactgg ccgtcgtttt acaacgtcgt
     3241 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3301 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3361 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt
     3421 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     3481 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttggtcc atttgtatgt
     3541 ccatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     3601 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     3661 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     3721 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     3781 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     3841 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     3901 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     3961 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     4021 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     4081 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
     4141 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
     4201 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
     4261 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
     4321 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
     4381 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
     4441 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
     4501 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
     4561 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
     4621 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
     4681 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
     4741 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
     4801 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
     4861 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
     4921 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
     4981 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
     5041 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
     5101 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
     5161 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
     5221 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
     5281 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
     5341 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
     5401 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
     5461 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
     5521 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
     5581 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
     5641 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
     5701 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
     5761 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
     5821 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     5881 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     5941 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     6001 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     6061 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     6121 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     6181 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     6241 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     6301 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     6361 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     6421 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     6481 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     6541 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     6601 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
     6661 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     6721 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     6781 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     6841 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     6901 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
     6961 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     7021 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     7081 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     7141 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     7201 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     7261 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     7321 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
     7381 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     7441 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     7501 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     7561 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7621 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7681 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7741 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7801 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8221 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8461 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
     8521 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     8581 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
     8641 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
     8701 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
     8761 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
     8821 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
     8881 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
     8941 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
     9001 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
     9061 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
     9121 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
     9181 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
     9241 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
     9301 cttcctcttt tctatagtat ttaaagatac cccaagaagc taattataac aagacgaact
     9361 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
     9421 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
     9481 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
     9541 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
     9601 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
     9661 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
     9721 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
     9781 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
     9841 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     9901 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     9961 tgggaactac tcacacatta ttatggagaa a
//
