LOCUS       sequence-232234-       11400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetOFF controlled GFP-tagging vector with Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 11400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1252_TetGFP(Hyg)
FEATURES             Location/Qualifiers
     source          1..11400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             633..653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      893..1145
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1795..2820)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    3485..3503
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    3691..3709
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             join(3854..3856,3857..3859,3860..4570)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3854..3856,3857..3859,3860..4570)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3899..3920)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4160..4179)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4507..4528
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4581..4597)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4581..4597)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4625..4642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4625..4641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4634..4656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4844..4868
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6169..6798
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7227..8300
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8366..8560
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8796..8818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8904..9044
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8959..8978
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9059..9076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9230..9818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9310..9329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9905..10699)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11124..11148
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11226..11400
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt catggcccgc ctggataagt caaaggtcat caactctgcc ttggagctgc
       61 tgaacgaggt cggcatcgag ggtctcacaa cgaggaagct ggcccagaag ctgggtgtcg
      121 agcagccaac cctctactgg cacgtcaaga acaagcgcgc cctcctggac gccctcgcca
      181 tcgagatgct ggaccgccac cacacccact tctgccccct ggagggcgag agctggcagg
      241 acttcctgcg caacaacgcc aagagcttca ggtgcgccct cctgagccac agggacggcg
      301 ccaaggtcca cctcggcacc cgccccaccg agaagcagta cgagaccctg gagaaccagc
      361 tcgccttcct gtgccagcag ggcttcagcc tggagaacgc cctctacgcc ctgagcgccg
      421 tcggtcactt caccctgggt tgcgtcctgg aggaccagga gcaccaggtc gccaaggaag
      481 agcgcgagac cccgaccacc gacagcatgc ccccgctcct gcgccaggcc atcgagctgt
      541 tcgaccacca gggtgccgag cccgccttcc tgttcggcct tgagcttatc atctgcggcc
      601 tggagaagca gctgaagtgc gagagcggct cgcccaagaa gaagcgcaag gtcgagggca
      661 gcgtcagcat gcagccaagc ctcaggagcg agtacgagta cccggtcttc agccacgtcc
      721 aggccggcat gttcagcccc gagctgcgca ccttcaccaa gggtgacgcc gagcgctggg
      781 tcagcggctc gccggccgac gccctggatg atttcgacct ggatatgctc cctgccgacg
      841 ccctggacga cttcgacctg gatatgctgt gaggcgcggc cggccgcccg gcgatcgttc
      901 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
      961 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     1021 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     1081 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     1141 agatccgatg ataagctgtc aaacatgaga attgcggccg cgtcgacaag aaggattacc
     1201 tctaaacaag tgtacctgtg cattctgggt aaacgactca taggagagtt gtaaaaaagt
     1261 ttcggccggc gtattgggtg ttacggagca ttcactaggc aaccatgcat ggttactatt
     1321 gtatacccat cttagtagga atgattttcg aggtttatac ctacgatgaa tgtgtgtcct
     1381 gtaggcttga gagttcaagg aagaaacagt gcaattatct ttgcgaaccc aggggctggt
     1441 gacggaattt tcatagtcaa gctatcagag taaagaagag gagcatgtca aagtacaatt
     1501 agagacaaat atatagtcgc gtggagccaa gagcggattc ctcagtctcg taggtctctt
     1561 gacgaccgtt gatctgcttg atctcgtctc ccgaaaatga aaatagactc tgctaagcta
     1621 ttcttctgct tcgccggagc ctgaagggcg tactagggtt gcgaggtcca atgcattaat
     1681 gcattgcaga tgagctgtat ctggaagagg taaacccgaa acgcatttta ttcttgttga
     1741 catggagcta ttaaatcact agaattactc gactggttcc ggtcggcatc tactctattt
     1801 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     1861 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     1921 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     1981 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
     2041 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     2101 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     2161 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     2221 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     2281 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     2341 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     2401 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     2461 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg
     2521 caacgtgaca ccctgtgaac ggcgggagat gcaataggtc aggctctcgc taaactcccc
     2581 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     2641 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     2701 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     2761 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     2821 ttggatgctt gggtagaata ggtaagtcag attgaatctg aaataaaggg aggaagggcg
     2881 aacttaagaa ggtatgaccg ggtcgtccac ttaccttgct tgacaaacgc accaagttat
     2941 cgtgcaccaa gcagcagatg ataataatgt cctcgttcct gtctgctaat aagagtcaca
     3001 cttcgagcgc cgccgctact gctacaagtg gggctgatct gaccagttgc ctaaatgaac
     3061 catcttgtca aacgacacaa attttgtgct caccgcctgg acgactaaac caaaataggc
     3121 attcattgtt gacctccact agctccagcc aagcccaaaa agtgctcctt caatatcctc
     3181 gactctagat aaatcattcc actcaacatt caggctcctc tgcgcacgta aagtgccaaa
     3241 ggcaataccc tgctcggtgg aatgccgccg ggcttgtcga ttttacgcac ataggcgcat
     3301 tcttgacttg aagcggagga gttcttcgtt gcgggttaca gtgttttaat aaaagaatgg
     3361 tcaaatcaaa ctgctagata tacctgtcag acactctagt tgttgacccc tatactctta
     3421 atacatcaga cagtacatgc atgttgcatg atgatgtcgg tacaactccc actagacata
     3481 tcctactcta tcattgatag agtgaccctt ccatagacat atcctactct atcattgata
     3541 gattgaccct tccactaggt tcacttttct ctatcattga tagggagtgg taaacaattg
     3601 atggacaaaa tctatcaatg atagagagtg catgttacct agtattgttc ttctctatca
     3661 ttgatagtga ctttgcctaa tcacttagca actctatcat tgatagagtt ctagtattag
     3721 caaggccttt cctcagatcc cgatcaatcc gacagccaaa cctcatccac tctcactttc
     3781 acactctaac ttatacgatc acttctcgcc cgttcttttg aacatcaaat caactacctt
     3841 acttctatca attatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3901 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3961 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     4021 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     4081 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     4141 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     4201 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     4261 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     4321 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     4381 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     4441 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     4501 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     4561 gctgtacaag ggtacccggg gatccactag ttctagagtc gacctgcagg catgcaagct
     4621 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4681 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4741 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg
     4801 atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc
     4861 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     4921 aaggtttatc cgttggtcca tttgtatgtc catgccaacc acagggttcc cctcgggatc
     4981 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     5041 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     5101 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     5161 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     5221 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     5281 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     5341 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     5401 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     5461 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     5521 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     5581 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     5641 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     5701 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     5761 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     5821 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     5881 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     5941 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     6001 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     6061 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     6121 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     6181 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     6241 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     6301 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     6361 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     6421 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     6481 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     6541 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     6601 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     6661 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     6721 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     6781 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     6841 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     6901 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     6961 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     7021 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     7081 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     7141 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     7201 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     7261 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     7321 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     7381 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     7441 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     7501 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     7561 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     7621 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     7681 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     7741 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     7801 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     7861 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     7921 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     7981 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     8041 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     8101 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     8161 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     8221 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     8281 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     8341 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     8401 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     8461 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     8521 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     8581 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     8641 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     8701 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     8761 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8821 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8881 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     8941 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9001 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     9061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9661 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
     9901 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
     9961 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    10021 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    10081 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    10141 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    10201 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    10261 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    10321 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    10381 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    10441 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    10501 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    10561 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    10621 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    10681 atattctcat tttagccatt tattatttcc ttcctctttt ctatagtatt taaagatacc
    10741 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    10801 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    10861 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    10921 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    10981 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    11041 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    11101 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt
    11161 agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattatgt
    11221 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
    11281 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
    11341 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
//
