LOCUS       sequence-232250-       11723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Introducing ectopic integration at the defined fungal ILV2-locus, 
            based on SRR (Sulfonylurea Resistance Reconstitution)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11723)
  AUTHORS   Patkar RN, Benke PI, Qu Z, Chen YY, Yang F, Swarup S, Naqvi NI
  TITLE     A fungal monooxygenase-derived jasmonate attenuates host innate 
            immunity.
  JOURNAL   Nat Chem Biol. 2015 Sep;11(9):733-40. doi: 10.1038/nchembio.1885. 
            Epub 2015 Aug 10.
  PUBMED    26258762
REFERENCE   2  (bases 1 to 11723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1885"; journalName: "Nat Chem Biol"; date: 
            "2015-09"; volume: "11"; issue: "9"; pages: "733-40"
COMMENT     Sequence Label: pFGL1010
FEATURES             Location/Qualifiers
     source          1..11723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(445..456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2681..2697)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2681..2697)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3610..3627)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3610..3626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3619..3641)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3829..3853
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5154..5783
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6212..7285
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7351..7545
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7781..7803)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7889..8029
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7944..7963
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8044..8061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8215..8803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8295..8314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10228..11022)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11447..11471
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11549..11723
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgacaatc acgagcgagc gaacaaacag gcagagttac aaacgccatc actacgcctt
       61 gtcttttcgt ctctgttacg tgtcgccacc cccatgcgag ctacacgtct gcgcctgaag
      121 tcacaaggct tgcaatttca tgacgatgaa caacactgac acttctgtta cagagcacct
      181 cgaactaagc ccagcgccag cttcaatgct cgccgcgatc cccagcctct ggtcaaccct
      241 cgctcaggtg aggcagacga atcgtaagtt gcgccaacac cgctcctaca ccgccaagac
      301 ccataccgcc gtcgcatttg gacaagcaca agactgattt gaccgtggca tagattcatt
      361 ggcaagacgg gaggagagat tttccacgag atgatgctga ggcaaaacgt caagcatatc
      421 tgtaagcgtc taattctaca aattcctacc ctcgatttca accacatatt caaccacata
      481 ttctgacaca tgagtcacag tcggttaccc tggcggtgct atccttcccg tgttcgacgc
      541 gatctacaac tcgaagcaca tcgactttgt tctgcccaag catgagcaag gcgccggcca
      601 catggcagag ggctatgctc gcgcttcagg caaacccggc gttgttctcg tcacctccgg
      661 ccccggtgcc acaaatgtca tcactcccat ggccgacgct cttgccgacg gtacacctct
      721 ggttgtattc tcaggacagg ttgttacctc tgatattgga agcgacgcct tccaggaggc
      781 cgacgtcata ggcatctccc ggtcttgcac caagtggaac gtcatggtta agagcgctga
      841 cgagctcccg aggagaatta acgaggcctt tgagattgcc accagtgggc gacctgggcc
      901 tgtcttggtc gatctgccca aggacgtcac ggctagtgtg ctgaggaggg ctatccccac
      961 cgagacctcg attccctcta ttagcgcagc agcacgggct gtccaagagg caggccgaaa
     1021 gcagcttgag cactccatca aacgcgtagc cgatctcgtc aacattgcca agaagcccgt
     1081 catatatgcc ggccaaggtg tcattttgtc ggaaggcggc gttgaacttc tcaaggcgct
     1141 tgccgacaag gcctcgattc ctgtcaccac cactctgcat ggtctgggag cctttgacga
     1201 gctcgacgag aaggcactgc acatgcttgg tatgcacggt tcggcttatg ccaacatgtc
     1261 catgcaagag gccgatttga tcattgccct tggtggccgc ttcgatgacc gtgtcactgg
     1321 cagcatcccc aaatttgctc ctgccgccaa gctagctgct gctgaaggac gcggaggtat
     1381 tgtccacttc gagattatgc ccaagaacat caacaaggtc gtccaagcaa cagaggccat
     1441 tgagggcgac gttgcttcga acttgaagct gttgctcccc aagattgaac aacgatccat
     1501 gaccgatcgc aaggagtggt tcgaccagat caaggagtgg aaggagaagt ggcctctgtc
     1561 acattatgag agggccgagc gtagtggtct catcaagcct cagactctga tcgaggagct
     1621 gagcaacctg actgctgacc gcaaggacat gacctacatc acaaccggtg ttggccagca
     1681 ccaaatgtgg acagcacaac atttcaggtg gaggcaccca cggtccatga tcacctctgg
     1741 cggtttggga accatgggat atggtctgcc ggcagcgatt ggcgccaagg ttgctaggcc
     1801 agatgctttg gtcattgaca tcgacggcga cgcatcgttc aacatgactc tgacagagct
     1861 ttcgacggcg gcacagttca acattggcgt caaggtcatt gtcttgaaca acgaggagca
     1921 gggaatggtg acccaatggc agaacttgtt ctacgaggac cgctactcac atacacacca
     1981 gcgcaaccca gacttcatga agctcgccga tgcaatggac gttcaacatc gccgtgtttc
     2041 gaagcctgac gatgtcgttg atgctctgac gtggctgatc aacaccgacg gccccgctct
     2101 gcttgaggtg atgactgata agaaggttcc tgttctgccc atggtgcccg gaggtaacgg
     2161 cctgcacgag ttcatcacgt ttgatgccag taagtaatat atccaatgca ctcctgtggc
     2221 ccattttgag ccttgggtat gattctagtt caacttgcta accagtgcat caactcgtat
     2281 aggcaaggat aagcaacgga gagagctgat gcgcgcgagg acgaatggcc tgcacggtta
     2341 agttgataat gggaattgat tattgcacgg gaatttgcat ctcgactaaa tcattccact
     2401 caacattcag gctcctctgc gcacgtaaag tgccaaaggc aataccctgc tcggtggaat
     2461 gccgccgggc ttgtcgattt tacgcacata tgcgcattct tgacttgaag cggaggagtt
     2521 cttcgttgcg ggttacagtg ttttaataaa agaatggtca aatcaaactg ctagatatac
     2581 ctgtcagaca ctctagttgt tgacccctat actcttaata catcagacag tacatgcatg
     2641 ttgcatgatg atgctcgaga attcgagctc ggtacccggg gatccactag ttctagagtc
     2701 gacctgcagg catgctccac agggacaaat accctccagg ggtaattcta ttttggacaa
     2761 aaagttggaa cttttgtgat accatggtgt taagaatgtc ttatggacaa ggggacgata
     2821 catttgagct gcgggaatgg ttagcggctt gtgtaaatat tgcatcattt tttttttctc
     2881 ttttactttt ttcttcttct tctggcttgg tattcgtgcg tttaattcgt ttcattcttc
     2941 tactttattc gaagcacatt ccttgcgtca tcagtatcat tgatacaagt gatgtttcca
     3001 actgaacaaa tgttacaaca ttggtccgtt ccagcctttg ttcatgagca gcaccggtat
     3061 ttgaacctcc aggttagagt agcctgggaa atgcaacgag cattctgcct aatactagct
     3121 gccactcgaa ggcatttata gttttgctga ggtttcaatg acgcccctct gaagacaaag
     3181 tagcacaacg gcattgacga gactactact ggtggttgaa ctgacttttg caccttgctg
     3241 ctgctctttg gaagaggctt gaccatccac tgcggaagag gtgcttgttg ccttgggatt
     3301 tacttacttg tccgtcatct tggcggactg ggccgagcaa ccaaccagca tcaaccttgg
     3361 tcgcagaaga cattttgaat cttggcaaag tgtgctatct attttgggcg tggaaatggt
     3421 tcttgagttt gttaggtggg ccctgggagg gatgcgcggt caactcctca cgactgcata
     3481 aatctaatgg agatgaaggt gtcgttggag aagttgcaga ttatatcggt gcatgaagca
     3541 tttgaacagt cccaaattat accactttca tcgatacaat tctgtcaatg actggttgtg
     3601 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3661 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3721 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
     3781 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
     3841 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     3901 gtgaaaaggt ttatccgttg gtccatttgt atgtccatgc caaccacagg gttcccctcg
     3961 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     4021 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     4081 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     4141 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     4201 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     4261 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     4321 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     4381 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     4441 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     4501 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     4561 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     4621 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     4681 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     4741 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     4801 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     4861 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     4921 gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc
     4981 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     5041 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     5101 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     5161 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     5221 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     5281 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     5341 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     5401 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     5461 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     5521 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     5581 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     5641 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     5701 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     5761 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     5821 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     5881 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     5941 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     6001 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     6061 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     6121 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     6181 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     6241 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     6301 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     6361 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     6421 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     6481 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     6541 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     6601 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     6661 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     6721 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     6781 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     6841 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     6901 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     6961 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     7021 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     7081 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
     7141 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
     7201 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
     7261 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
     7321 aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa
     7381 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
     7441 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
     7501 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
     7561 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
     7621 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
     7681 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
     7741 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7801 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7861 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     7921 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     7981 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     8041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     8101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     8161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     8221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8641 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggctga tgaatcccct
     8881 aatgattttg gtaaaaatca ttaagttaag gtggatacac atcttgtcat atgatcaaat
     8941 ggtttcgcga aaaatcaata atcagacaac aagatgtgcg aactcgatat tttacacgac
     9001 tctctttacc aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct
     9061 tgccacgcct tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca
     9121 accaaagcga gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac
     9181 ctccacaaag agcgactcgc tgtataccgt tggcatgcta gctttatctg ttcgggcaat
     9241 acgatgccca ttgtacttgt tgactggtct gatattcgtg agcaaaaacg acttatggta
     9301 ttgcgagctt cagtcgcact acacggtcgt tctgttactc tttatgagaa agcgttcccg
     9361 ctttcagagc aatgttcaaa gaaagctcat gaccaatttc tagccgacct tgcgagcatt
     9421 ctaccgagta acaccacacc gctcattgtc agtgatgctg gctttaaagt gccatggtat
     9481 aaatccgttg agaagctggg ttggtactgg ttaagtcgag taagaggaaa agtacaatat
     9541 gcagacctag gagcggaaaa ctggaaacct atcagcaact tacatgatat gtcatctagt
     9601 cactcaaaga ctttaggcta taagaggctg actaaaagca atccaatctc atgccaaatt
     9661 ctattgtata aatctcgctc taaaggccga aaaaatcagc gctcgacacg gactcattgt
     9721 caccacccgt cacctaaaat ctactcagcg tcggcaaagg agccatgggt tctagcaact
     9781 aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg
     9841 cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat
     9901 agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa
     9961 ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag
    10021 gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg
    10081 cggcattctg gctacacaat aacaagggaa gacttactcg tggctgcaac cctactagct
    10141 caaaatttat tcacacatgg ttacgctttg gggaaattat gaggggatct ctcagcgtta
    10201 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
    10261 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    10321 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    10381 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    10441 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    10501 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    10561 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    10621 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    10681 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    10741 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    10801 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    10861 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    10921 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    10981 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    11041 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    11101 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    11161 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    11221 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    11281 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    11341 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    11401 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11461 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    11521 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    11581 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    11641 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    11701 actcacacat tattatggag aaa
//
