LOCUS       sequence-232262-        4100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mNeptune2 promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-mNeptune2-nat
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    2026..2044
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(2110..2127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2281..2869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2361..2380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3040..3831,3832..3900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3663..3682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3901..4005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4073..4091
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgaattct aaggaggaaa aaaaatggtg agtaaaggcg aagaactgat
      421 caaagaaaac atgcacatga aactgtatat ggaaggcact gtgaacaacc accactttaa
      481 atgcaccagc gaaggtgaag gtaaacccta tgagggcacc cagacaggcc gtattaaggt
      541 tgtggagggt ggcccgctgc cttttgcttt cgacatcttg gcaacctgct ttatgtacgg
      601 ttctaaaacc ttcattaacc atacccaggg tattcctgac tttttcaaac agtctttccc
      661 ggagggcttc acgtgggaac gcgtaacgac ctatgaggac ggtggtgttc tgaccgtaac
      721 gcaggatacc agcctccagg acggctgcct gatttataat gttaaactgc gtggcgttaa
      781 cttcccctcc aatggcccag tgatgcagaa aaagaccctc ggctgggaag cctcgaccga
      841 aaccctttat ccggccgacg gtggtcttga gggccgttgc gacatggccc ttaaacttgt
      901 gggcggcggt catcttcatt gcaacctcaa aaccacctat cgtagcaaaa aaccggctaa
      961 aaacctcaag atgcctggag tgtatttcgt tgaccgccgc ctggaacgca ttaaggaagc
     1021 agataacgag acctatgtgg aacagcatga agtcgcggtc gcacgttact gcgatctgcc
     1081 gtccaaactg ggtcataaac ttaacggcat ggacgaactt tacaaataag tcgacggagg
     1141 acagtaatgg gtacgaccct tgatgacacg gcttatcggt atcgcacctc tgtcccgggt
     1201 gacgccgaag ccatcgaagc actggatggg tccttcacca ccgacaccgt cttccgtgtc
     1261 accgcaaccg gtgacggctt caccctgcgg gaggtgccgg ttgacccgcc cctgaccaag
     1321 gtgtttccgg acgacgaaag cgatgacgaa tcggacgatg gtgaagacgg tgatccggac
     1381 tcccggacgt tcgtcgcgta tggtgacgat ggcgacctgg caggcttcgt ggtcgtcagc
     1441 tatagcggct ggaaccgccg tctgaccgtc gaagacatcg aagtcgcacc agaacaccgt
     1501 ggtcacggtg tcggtcgtgc attgatgggt ttagcaacgg aatttgcccg tgaacgtggt
     1561 gcaggtcacc tctggctgga agtcaccaac gtcaacgcac cggcgatcca cgcgtatcgt
     1621 cgtatggggt tcaccctctg cggcctggac accgccctgt acgacggcac ggccagcgat
     1681 ggcgaacagg cgctctacat gagcatgccc tgcccgtaaa agctttaaaa gccttatata
     1741 ttcttttttt tcttataaaa cttaaaacct tagaggctat ttaagttgct gatttatatt
     1801 aattttattg ttcaaacatg agagcttagt acgtgaaaca tgagagctta gtacgttagc
     1861 catgagagct tagtacgtta gccatgaggg tttagttcgt taaacatgag agcttagtac
     1921 gttaaacatg agagcttagt acgtgaaaca tgagagctta gtacgtacta tcaacaggtt
     1981 gaactgccaa cgactacgca ctagccaaca agagcttcag ggttgagatg tgtataagag
     2041 acagctgtct taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4021 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4081 tatcacgagg ccctttcgtc
//
