LOCUS       sequence-232267-        4254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mNeptune2 labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-proD/mNeptune2-nat
FEATURES             Location/Qualifiers
     source          1..4254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    2180..2198
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(2264..2281)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2435..3023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2515..2534)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3194..3985,3986..4054))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3817..3836
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4055..4159)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4227..4245
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg cgcgaattcc acagctaaca ccacgtcgtc cctatctgct gccctaggtc
      421 tatgggtggt tgctggataa ctttacgggc atgcataagg ctcgtataat atattcaggg
      481 agaccacaac ggtttccctc tacaaataat tttgtttaac tttgaattcg cgctaaggag
      541 gaaaaaaaat ggtgagtaaa ggcgaagaac tgatcaaaga aaacatgcac atgaaactgt
      601 atatggaagg cactgtgaac aaccaccact ttaaatgcac cagcgaaggt gaaggtaaac
      661 cctatgaggg cacccagaca ggccgtatta aggttgtgga gggtggcccg ctgccttttg
      721 ctttcgacat cttggcaacc tgctttatgt acggttctaa aaccttcatt aaccataccc
      781 agggtattcc tgactttttc aaacagtctt tcccggaggg cttcacgtgg gaacgcgtaa
      841 cgacctatga ggacggtggt gttctgaccg taacgcagga taccagcctc caggacggct
      901 gcctgattta taatgttaaa ctgcgtggcg ttaacttccc ctccaatggc ccagtgatgc
      961 agaaaaagac cctcggctgg gaagcctcga ccgaaaccct ttatccggcc gacggtggtc
     1021 ttgagggccg ttgcgacatg gcccttaaac ttgtgggcgg cggtcatctt cattgcaacc
     1081 tcaaaaccac ctatcgtagc aaaaaaccgg ctaaaaacct caagatgcct ggagtgtatt
     1141 tcgttgaccg ccgcctggaa cgcattaagg aagcagataa cgagacctat gtggaacagc
     1201 atgaagtcgc ggtcgcacgt tactgcgatc tgccgtccaa actgggtcat aaacttaacg
     1261 gcatggacga actttacaaa taagtcgacg gaggacagta atgggtacga cccttgatga
     1321 cacggcttat cggtatcgca cctctgtccc gggtgacgcc gaagccatcg aagcactgga
     1381 tgggtccttc accaccgaca ccgtcttccg tgtcaccgca accggtgacg gcttcaccct
     1441 gcgggaggtg ccggttgacc cgcccctgac caaggtgttt ccggacgacg aaagcgatga
     1501 cgaatcggac gatggtgaag acggtgatcc ggactcccgg acgttcgtcg cgtatggtga
     1561 cgatggcgac ctggcaggct tcgtggtcgt cagctatagc ggctggaacc gccgtctgac
     1621 cgtcgaagac atcgaagtcg caccagaaca ccgtggtcac ggtgtcggtc gtgcattgat
     1681 gggtttagca acggaatttg cccgtgaacg tggtgcaggt cacctctggc tggaagtcac
     1741 caacgtcaac gcaccggcga tccacgcgta tcgtcgtatg gggttcaccc tctgcggcct
     1801 ggacaccgcc ctgtacgacg gcacggccag cgatggcgaa caggcgctct acatgagcat
     1861 gccctgcccg taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa
     1921 accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct
     1981 tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg
     2041 agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga
     2101 aacatgagag cttagtacgt actatcaaca ggttgaactg ccaacgacta cgcactagcc
     2161 aacaagagct tcagggttga gatgtgtata agagacagct gtcttaatga atcggccaac
     2221 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     2641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4201 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
