LOCUS       sequence-232268-        3902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mNeptune2 promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-mNeptune2-Sh_ble
FEATURES             Location/Qualifiers
     source          1..3902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    1828..1846
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1912..1929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2083..2671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2163..2182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2842..3633,3634..3702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3465..3484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3703..3807)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3875..3893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgaattct aaggaggaaa aaaaatggtg agtaaaggcg aagaactgat
      421 caaagaaaac atgcacatga aactgtatat ggaaggcact gtgaacaacc accactttaa
      481 atgcaccagc gaaggtgaag gtaaacccta tgagggcacc cagacaggcc gtattaaggt
      541 tgtggagggt ggcccgctgc cttttgcttt cgacatcttg gcaacctgct ttatgtacgg
      601 ttctaaaacc ttcattaacc atacccaggg tattcctgac tttttcaaac agtctttccc
      661 ggagggcttc acgtgggaac gcgtaacgac ctatgaggac ggtggtgttc tgaccgtaac
      721 gcaggatacc agcctccagg acggctgcct gatttataat gttaaactgc gtggcgttaa
      781 cttcccctcc aatggcccag tgatgcagaa aaagaccctc ggctgggaag cctcgaccga
      841 aaccctttat ccggccgacg gtggtcttga gggccgttgc gacatggccc ttaaacttgt
      901 gggcggcggt catcttcatt gcaacctcaa aaccacctat cgtagcaaaa aaccggctaa
      961 aaacctcaag atgcctggag tgtatttcgt tgaccgccgc ctggaacgca ttaaggaagc
     1021 agataacgag acctatgtgg aacagcatga agtcgcggtc gcacgttact gcgatctgcc
     1081 gtccaaactg ggtcataaac ttaacggcat ggacgaactt tacaaataag tcgacggagg
     1141 acagtaatgg ccaagttgac cagtgccgtt ccggtgctga ccgcacgtga cgtcgccgga
     1201 gcagtggagt tctggaccga ccgtctgggg ttctcccgtg acttcgtgga ggacgacttc
     1261 gccggtgtgg tccgtgacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     1321 ccggacaaca ccctggcctg ggtgtgggtg cgtggcctgg acgagctgta cgccgagtgg
     1381 agcgaagtcg tgtccacgaa cttccgtgac gcctccgggc cggccatgac cgagatcggc
     1441 gagcagccgt gggggcgtga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     1501 gtggccgagg agcaggactg aaagctttaa aagccttata tattcttttt tttcttataa
     1561 aacttaaaac cttagaggct atttaagttg ctgatttata ttaattttat tgttcaaaca
     1621 tgagagctta gtacgtgaaa catgagagct tagtacgtta gccatgagag cttagtacgt
     1681 tagccatgag ggtttagttc gttaaacatg agagcttagt acgttaaaca tgagagctta
     1741 gtacgtgaaa catgagagct tagtacgtac tatcaacagg ttgaactgcc aacgactacg
     1801 cactagccaa caagagcttc agggttgaga tgtgtataag agacagctgt cttaatgaat
     1861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2521 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3841 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3901 tc
//
