LOCUS       sequence-232284-        4076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mWasabi promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-mWasabi-nat
FEATURES             Location/Qualifiers
     source          1..4076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    2002..2020
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(2086..2103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2257..2845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2337..2356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3016..3807,3808..3876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3639..3658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3877..3981)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4049..4067
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgaattct aaggaggaaa aaaaatggtg agtaaaggcg aagaaaccac
      421 catgggcgtg attaaaccag atatgaaaat caagctgaaa atggaaggta atgttaacgg
      481 ccatgcattt gtaattgaag gcgagggaga gggcaagccg tatgatggta cgaatacgat
      541 caatctggag gttaaggaag gcgcgccttt acccttttcc tatgatatcc tgaccaccgc
      601 cttttcgtat ggtaatcgcg cattcacgaa atatccggac gatatcccga actatttcaa
      661 acagagcttt ccggaaggtt atagctggga acgtaccatg accttcgagg ataagggcat
      721 cgtgaaagtg aaaagcgata tttccatgga agaagattct ttcatctacg agatccatct
      781 gaaaggcgaa aacttcccgc caaacggtcc agtgatgcag aaagagacga cgggctggga
      841 tgcgagcacg gagcgtatgt atgttcgcga cggcgtgctg aagggtgatg tcaagatgaa
      901 actcctgctg gaaggcggtg gccaccaccg tgtggatttc aagacgattt accgcgccaa
      961 gaaggcggtc aaactgccag actaccactt tgtggaccat cgtattgaaa tccttaatca
     1021 tgataaagac tataacaaag tgaccgtata cgagattgca gtggcacgca attccacgga
     1081 cggcatggac gaactttaca aataagtcga cggaggacag taatgggtac gacccttgat
     1141 gacacggctt atcggtatcg cacctctgtc ccgggtgacg ccgaagccat cgaagcactg
     1201 gatgggtcct tcaccaccga caccgtcttc cgtgtcaccg caaccggtga cggcttcacc
     1261 ctgcgggagg tgccggttga cccgcccctg accaaggtgt ttccggacga cgaaagcgat
     1321 gacgaatcgg acgatggtga agacggtgat ccggactccc ggacgttcgt cgcgtatggt
     1381 gacgatggcg acctggcagg cttcgtggtc gtcagctata gcggctggaa ccgccgtctg
     1441 accgtcgaag acatcgaagt cgcaccagaa caccgtggtc acggtgtcgg tcgtgcattg
     1501 atgggtttag caacggaatt tgcccgtgaa cgtggtgcag gtcacctctg gctggaagtc
     1561 accaacgtca acgcaccggc gatccacgcg tatcgtcgta tggggttcac cctctgcggc
     1621 ctggacaccg ccctgtacga cggcacggcc agcgatggcg aacaggcgct ctacatgagc
     1681 atgccctgcc cgtaaaagct ttaaaagcct tatatattct tttttttctt ataaaactta
     1741 aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca aacatgagag
     1801 cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt acgttagcca
     1861 tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag cttagtacgt
     1921 gaaacatgag agcttagtac gtactatcaa caggttgaac tgccaacgac tacgcactag
     1981 ccaacaagag cttcagggtt gagatgtgta taagagacag ctgtcttaat gaatcggcca
     2041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4021 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
