LOCUS       sequence-232285-        5619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Kelley JB, Talley AM, Spencer A, Gioeli D, Paschal BM
  TITLE     Karyopherin alpha7 (KPNA7), a divergent member of the importin alpha
            family of nuclear import receptors.
  JOURNAL   BMC Cell Biol. 2010 Aug 11;11:63. doi: 10.1186/1471-2121-11-63.
  PUBMED    20701745
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Cell 
            Biol."; date: "2010-08-11"; volume: "11:63. doi"; pages: " 
            10.1186/1471-2121-11-63"
COMMENT     Sequence Label: pCMVTNT-T7-KPNA7
FEATURES             Location/Qualifiers
     source          1..5619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        143..522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        523..726
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     677..697
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     723..747
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          862..994
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     977..996
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1039..1057
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1112..1121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2345..2354
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2738..2859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2749..2768)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2803..2822
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3040..3495
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3127..3146)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3337..3358
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3562..3581)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3681..3703
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3741..3759)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3827..3931
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3932..4000,4001..4792)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4150..4169)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4963..5551
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5452..5471
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt
       61 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
      121 tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata gtaatcaatt
      181 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      241 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      301 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      361 actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc
      421 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct
      481 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      541 tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      601 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat
      661 aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      721 agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt ttatcacagt
      781 taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag ctgcagaagt
      841 tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
      901 tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt
      961 cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt caattacagc
     1021 tcttaaggct agagtactta atacgactca ctataggcta gcatttaggt gacactatag
     1081 aatacaagct acttgttctt tttgcactcg agccaccatg gccagcatga ccggcggcca
     1141 gcagatgggc gaattcccga ccttagatgc tccagaagag aggcggagaa aatttaagta
     1201 ccgaggcaaa gatgtgtctc tgaggcgaca gcagaggatg gcggtcagtc tggagctccg
     1261 aaaggccaag aaagatgaac agaccttaaa gagaaggaat atcacgagct tctgccctga
     1321 cacaccttct gaaaaaacag ccaaaggggt ggcggtcagc ctcactctgg gtgaaataat
     1381 caaaggtgtg aatagctcag atccagtcct atgtttccag gccacccaga cagccaggaa
     1441 aatgctatcc caggaaaaga acccccctct gaaactggtc attgaagcgg gcctcattcc
     1501 caggatggtg gagttcctga agtcatcact ttacccctgc ttgcagtttg aggctgcctg
     1561 ggccctgacc aacatcgctt cagggacttc ggagcagact cgtgccgtgg tagaaggggg
     1621 agccatccag cccttgattg agctcctgtc ttcctccaac gtggctgtgt gtgaacaggc
     1681 agtgtgggct cttggtaata tagccggtga tggcccagag ttcagagata acgtcatcac
     1741 aagcaatgcc atcccacatc tcctagcctt gatttcaccc accctgccga tcacatttct
     1801 gcggaacatc acgtggacct tgtcgaatct gtgccgaaac aagaacccat acccttgcga
     1861 cactgcggtg aagcagatac tgccggccct ccttcacctc ctgcagcacc aggacagtga
     1921 ggttctctcg gatgcctgct gggcactgtc ctacctcacc gacggctcca acaagcgcat
     1981 cggccaagtg gttaacacgg gggtcctgcc caggctggta gtgctcatga ccagctcaga
     2041 actcaatgtc ttgactcctt ctctccgcac cgtggggaac attgtcacag gcacagatga
     2101 gcagacgcag atggccattg atgcgggtat gctgaacgtg ctcccccagc tcctgcaaca
     2161 caacaagccc tccatccaga aggaggcagc ctgggccctg agcaacgtag cagcggggcc
     2221 ttgtcaccac atccagcagc tgcttgccta cgacgtcttg cctcccttgg tggctctgct
     2281 aaaaaacgga gaatttaaag tccagaaaga ggctgtctgg atggtggcga actttgcaac
     2341 aggggccacc atggatcagc tgatccagct cgtccactct ggggtcctgg agccactggt
     2401 gaatctgctc actgccccag atgttaaaat tgtcctcatc atccttgatg tcatctcttg
     2461 catcctccag gcggcagaga aacggtctga gaaggaaaac ctgtgtcttc tgatagaaga
     2521 acttggtggg atcgatagaa ttgaggcttt acagctgcat gagaaccgtc aaattggcca
     2581 gtcggctttg aacatcatcg agaagcactt tggtgaggaa gaagatgaga gccaaacttt
     2641 actgagccaa gtcatagacc aagattatga atttatagat tatgaatgct tagcaaaaaa
     2701 atagctcgac ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga
     2761 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2821 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2881 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     2941 aaatgtggta aaatcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat
     3001 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat
     3061 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3121 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3181 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3241 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3301 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3361 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3421 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3481 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3541 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     3601 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     3661 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     3721 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     3781 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3841 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3901 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3961 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4021 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4081 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4141 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4201 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4261 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4321 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4381 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4441 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4501 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4561 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4621 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4681 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4741 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4801 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5101 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5581 cctggccttt tgctggcctt ttgctcacat ggctcgaca
//
