LOCUS       sequence-23230-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Yeung T, Terebiznik M, Yu L, Silvius J, Abidi WM, Philips M, Levine 
            T, Kapus A, Grinstein S
  TITLE     Receptor activation alters inner surface potential during 
            phagocytosis.
  JOURNAL   Science. 2006 Jul 21. 313(5785):347-51.
  PUBMED    16857939
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2006 Jul 21. 313(5785):347-51."
COMMENT     Sequence Label: R-pre-GFP
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    231..352
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(268..287)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     322..341
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(359..814)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(496..517)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     708..727
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        841..945
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggacgagct gtacaagtcc ggactcagat ctcgaggaag gatggccaga gatggtcggc
       61 gcaggagacg gcgcgctagg gccagatgtg taattatgta aggatccacc ggatctagat
      121 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      181 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      241 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      301 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
      361 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
      421 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
      481 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
      541 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
      601 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
      661 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
      721 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
      781 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
      841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctg
//
