LOCUS       sequence-232301-        8392 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base editor made from APOBEC3C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris 
            RS
  TITLE     A panel of eGFP reporters for single base editing by APOBEC-Cas9 
            editosome complexes.
  JOURNAL   Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
  PUBMED    30679582
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-01-24"; volume: "9(1):497. doi"; pages: " 
            10.1038/s41598-018-36739-9"
COMMENT     Sequence Label: A3C-Cas9n-UGI
FEATURES             Location/Qualifiers
     source          1..8392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(1004..4402,4403..4405,4406..5002,5003,5004..5006,
                     5007..5008,5009..5011,5012..5104)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1004..1027,1028..1030,1031..5104)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5117..5365
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5378..5398
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5407..5424
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5447..5464)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5453..5677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5748..5764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5748..5764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5761..5783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5772..5788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5796..5802,5803..5820,5821..5826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5841..5862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5979..5996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6150..6738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6230..6249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6909..7700,7701..7769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7532..7551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7770..7874)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7953..7972)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8144..8392,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgaa tccacagatc agaaacccga tgaaggcaat
      421 gtatccaggc acattctact tccaatttaa aaacctatgg gaagccaacg atcggaacga
      481 aacttggctg tgcttcaccg tggaaggtat aaagcgccgc tcagttgtct cctggaagac
      541 gggcgtcttc cgaaaccagg tggattctga gacccattgt catgcagaaa ggtgcttcct
      601 ctcttggttc tgcgacgaca tactgtctcc taacacaaag taccaggtca cctggtacac
      661 atcttggagc ccttgcccag actgtgcagg ggaggtggcc gagttcctgg ccaggcacag
      721 caacgtgaat ctcaccatct tcaccgcccg cctctactac ttccagtatc catgttacca
      781 ggaggggctc cgcagcctga gtcaagaagg ggtcgctgtg gagatcatgg actatgaaga
      841 ttttaaatat tgttgggaaa actttgtgta caatgataat gagccattca agccttggaa
      901 gggattaaaa accaactttc gacttctgaa aagaaggcta cgggagagtc tccagagcgg
      961 cagcgagact cccgggacct cagagtccgc cacacccgaa agtgataaaa agtattctat
     1021 tggtttagcc atcggcacta attccgttgg atgggctgtc ataaccgatg aatacaaagt
     1081 accttcaaag aaatttaagg tgttggggaa cacagaccgt cattcgatta aaaagaatct
     1141 tatcggtgcc ctcctattcg atagtggcga aacggcagag gcgactcgcc tgaaacgaac
     1201 cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt tacttacaag aaatttttag
     1261 caatgagatg gccaaagttg acgattcttt ctttcaccgt ttggaagagt ccttccttgt
     1321 cgaagaggac aagaaacatg aacggcaccc catctttgga aacatagtag atgaggtggc
     1381 atatcatgaa aagtacccaa cgatttatca cctcagaaaa aagctagttg actcaactga
     1441 taaagcggac ctgaggttaa tctacttggc tcttgcccat atgataaagt tccgtgggca
     1501 ctttctcatt gagggtgatc taaatccgga caactcggat gtcgacaaac tgttcatcca
     1561 gttagtacaa acctataatc agttgtttga agagaaccct ataaatgcaa gtggcgtgga
     1621 tgcgaaggct attcttagcg cccgcctctc taaatcccga cggctagaaa acctgatcgc
     1681 acaattaccc ggagagaaga aaaatgggtt gttcggtaac cttatagcgc tctcactagg
     1741 cctgacacca aattttaagt cgaacttcga cttagctgaa gatgccaaat tgcagcttag
     1801 taaggacacg tacgatgacg atctcgacaa tctactggca caaattggag atcagtatgc
     1861 ggacttattt ttggctgcca aaaaccttag cgatgcaatc ctcctatctg acatactgag
     1921 agttaatact gagattacca aggcgccgtt atccgcttca atgatcaaaa ggtacgatga
     1981 acatcaccaa gacttgacac ttctcaaggc cctagtccgt cagcaactgc ctgagaaata
     2041 taaggaaata ttctttgatc agtcgaaaaa cgggtacgca ggttatattg acggcggagc
     2101 gagtcaagag gaattctaca agtttatcaa acccatatta gagaagatgg atgggacgga
     2161 agagttgctt gtaaaactca atcgcgaaga tctactgcga aagcagcgga ctttcgacaa
     2221 cggtagcatt ccacatcaaa tccacttagg cgaattgcat gctatactta gaaggcagga
     2281 ggatttttat ccgttcctca aagacaatcg tgaaaagatt gagaaaatcc taacctttcg
     2341 cataccttac tatgtgggac ccctggcccg agggaactct cggttcgcat ggatgacaag
     2401 aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa gttgtcgata aaggtgcgtc
     2461 agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag aatttaccga acgaaaaagt
     2521 attgcctaag cacagtttac tttacgagta tttcacagtg tacaatgaac tcacgaaagt
     2581 taagtatgtc actgagggca tgcgtaaacc cgcctttcta agcggagaac agaagaaagc
     2641 aatagtagat ctgttattca agaccaaccg caaagtgaca gttaagcaat tgaaagagga
     2701 ctactttaag aaaattgaat gcttcgattc tgtcgagatc tccggggtag aagatcgatt
     2761 taatgcgtca cttggtacgt atcatgacct cctaaagata attaaagata aggacttcct
     2821 ggataacgaa gagaatgaag atatcttaga agatatagtg ttgactctta ccctctttga
     2881 agatcgggaa atgattgagg aaagactaaa aacatacgct cacctgttcg acgataaggt
     2941 tatgaaacag ttaaagaggc gtcgctatac gggctgggga cgattgtcgc ggaaacttat
     3001 caacgggata agagacaagc aaagtggtaa aactattctc gattttctaa agagcgacgg
     3061 cttcgccaat aggaacttta tgcagctgat ccatgatgac tctttaacct tcaaagagga
     3121 tatacaaaag gcacaggttt ccggacaagg ggactcattg cacgaacata ttgcgaatct
     3181 tgctggttcg ccagccatca aaaagggcat actccagaca gtcaaagtag tggatgagct
     3241 agttaaggtc atgggacgtc acaaaccgga aaacattgta atcgagatgg cacgcgaaaa
     3301 tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg atgaagagaa tagaagaggg
     3361 tattaaagaa ctgggcagcc agatcttaaa ggagcatcct gtggaaaata cccaattgca
     3421 gaacgagaaa ctttacctct attacctaca aaatggaagg gacatgtatg ttgatcagga
     3481 actggacata aaccgtttat ctgattacga cgtcgatcac attgtacccc aatccttttt
     3541 gaaggacgat tcaatcgaca ataaagtgct tacacgctcg gataagaacc gagggaaaag
     3601 tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag aactattggc ggcagctcct
     3661 aaatgcgaaa ctgataacgc aaagaaagtt cgataactta actaaagctg agaggggtgg
     3721 cttgtctgaa cttgacaagg ccggatttat taaacgtcag ctcgtggaaa cccgccaaat
     3781 cacaaagcat gttgcacaga tactagattc ccgaatgaat acgaaatacg acgagaacga
     3841 taagctgatt cgggaagtca aagtaatcac tttaaagtca aaattggtgt cggacttcag
     3901 aaaggatttt caattctata aagttaggga gataaataac taccaccatg cgcacgacgc
     3961 ttatcttaat gccgtcgtag ggaccgcact cattaagaaa tacccgaagc tagaaagtga
     4021 gtttgtgtat ggtgattaca aagtttatga cgtccgtaag atgatcgcga aaagcgaaca
     4081 ggagataggc aaggctacag ccaaatactt cttttattct aacattatga atttctttaa
     4141 gacggaaatc actctggcaa acggagagat acgcaaacga cctttaattg aaaccaatgg
     4201 ggagacaggt gaaatcgtat gggataaggg ccgggacttc gcgacggtga gaaaagtttt
     4261 gtccatgccc caagtcaaca tagtaaagaa aactgaggtg cagaccggag ggttttcaaa
     4321 ggaatcgatt cttccaaaaa ggaatagtga taagctcatc gctcgtaaaa aggactggga
     4381 cccgaaaaag tacggtggct tcgatagccc tacagttgcc tattctgtcc tagtagtggc
     4441 aaaagttgag aagggaaaat ccaagaaact gaagtcagtc aaagaattat tggggataac
     4501 gattatggag cgctcgtctt ttgaaaagaa ccccatcgac ttccttgagg cgaaaggtta
     4561 caaggaagta aaaaaggatc tcataattaa actaccaaag tatagtctgt ttgagttaga
     4621 aaatggccga aaacggatgt tggctagcgc cggagagctt caaaagggga acgaactcgc
     4681 actaccgtct aaatacgtga atttcctgta tttagcgtcc cattacgaga agttgaaagg
     4741 ttcacctgaa gataacgaac agaagcaact ttttgttgag cagcacaaac attatctcga
     4801 cgaaatcata gagcaaattt cggaattcag taagagagtc atcctagctg atgccaatct
     4861 ggacaaagta ttaagcgcat acaacaagca cagggataaa cccatacgtg agcaggcgga
     4921 aaatattatc catttgttta ctcttaccaa cctcggcgct ccagccgcat tcaagtattt
     4981 tgacacaacg atagatcgca aacgatacac ttctaccaag gaggtgctag acgcgacact
     5041 gattcaccaa tccatcacgg gattatatga aactcggata gatttgtcac agcttggggg
     5101 tgactctggt ggttctacta atctgtcaga tattattgaa aaggagaccg gtaagcaact
     5161 ggttatccag gaatccatcc tcatgctccc agaggaggtg gaagaagtca ttgggaacaa
     5221 gccggaaagc gatatactcg tgcacaccgc ctacgacgag agcaccgacg agaatgtcat
     5281 gcttctgact agcgacgccc ctgaatacaa gccttgggct ctggtcatac aggatagcaa
     5341 cggtgagaac aagattaaga tgctctctgg tggttctccc aagaagaaga ggaaagtcta
     5401 accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct
     5461 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     5521 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     5581 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     5641 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     5701 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5761 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5821 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5881 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5941 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6001 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6061 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6121 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6181 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6241 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6301 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6361 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6421 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6481 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6541 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6601 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6661 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6721 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6781 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6841 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6901 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6961 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7021 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7081 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7141 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7201 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7261 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7321 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7381 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7441 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7501 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7561 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7621 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7681 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7741 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7801 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     7921 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     7981 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8041 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     8101 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     8161 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     8221 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8281 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8341 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tc
//
