LOCUS       sequence-232319-        8941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base editor made from APOBEC3F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris 
            RS
  TITLE     A panel of eGFP reporters for single base editing by APOBEC-Cas9 
            editosome complexes.
  JOURNAL   Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
  PUBMED    30679582
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-01-24"; volume: "9(1):497. doi"; pages: " 
            10.1038/s41598-018-36739-9"
COMMENT     Sequence Label: A3F-Cas9n-UGI
FEATURES             Location/Qualifiers
     source          1..8941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(1553..4951,4952..4954,4955..5551,5552,5553..5555,
                     5556..5557,5558..5560,5561..5653)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1553..1576,1577..1579,1580..5653)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5666..5914
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5927..5947
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5956..5973
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5996..6013)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6002..6226
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6297..6313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6297..6313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6310..6332)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6321..6337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6345..6351,6352..6369,6370..6375))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6390..6411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6528..6545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6699..7287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6779..6798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7458..8249,8250..8318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8081..8100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8319..8423)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8502..8521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8693..8941,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgaa gcctcacttc agaaacacag tggagcgaat
      421 gtatcgagac acattctcct acaactttta taatagaccc atcctttctc gtcggaatac
      481 cgtctggctg tgctacgaag tgaaaacaaa gggtccctca aggccccgtt tggacgcaaa
      541 gatctttcga ggccaggtgt attcccagcc tgagcaccac gcagaaatgt gcttcctctc
      601 ttggttctgt ggcaaccagc tgcctgctta caagtgtttc cagatcacct ggtttgtatc
      661 ctggaccccc tgcccggact gtgtggcgaa gctggccgaa ttcctggctg agcaccccaa
      721 tgtcaccctg accatctccg ccgcccgcct ctactactac tgggaaagag attaccgaag
      781 ggcgctctgc aggctgagtc aggcaggggc ccgcgtgaag attatggacg atgaagaatt
      841 tgcatactgc tgggaaaact ttgtgtacag tgaaggtcag ccattcatgc cttggtacaa
      901 attcgatgac aattatgcat tcctgcaccg cacgctaaag gagattctca gaaacccgat
      961 ggaggcaatg tatccacaca tattctactt ccactttaaa aacctacgca aagcctatgg
     1021 tcggaacgaa agctggctgt gcttcaccat ggaagttgta aagcaccact cacctgtctc
     1081 ctggaagagg ggcgtcttcc gaaaccaggt ggatcctgag acccattgtc atgcagaaag
     1141 gtgcttcctc tcttggttct gtgacgacat actgtctcct aacacaaact acgaggtcac
     1201 ctggtacaca tcttggagcc cttgcccaga gtgtgcaggg gaggtggccg agttcctggc
     1261 caggcacagc aacgtgaatc tcaccatctt caccgcccgc ctctactact tctgggatac
     1321 agattaccag gaggggctcc gcagcctgag tcaggaaggg gcctccgtgg agatcatggg
     1381 ctacaaagat tttaaatatt gttgggaaaa ctttgtgtac aatgatgatg agccattcaa
     1441 gccttggaaa ggactaaaat acaactttct attcctggac agcaagctgc aggagattct
     1501 cgagagcggc agcgagactc ccgggacctc agagtccgcc acacccgaaa gtgataaaaa
     1561 gtattctatt ggtttagcca tcggcactaa ttccgttgga tgggctgtca taaccgatga
     1621 atacaaagta ccttcaaaga aatttaaggt gttggggaac acagaccgtc attcgattaa
     1681 aaagaatctt atcggtgccc tcctattcga tagtggcgaa acggcagagg cgactcgcct
     1741 gaaacgaacc gctcggagaa ggtatacacg tcgcaagaac cgaatatgtt acttacaaga
     1801 aatttttagc aatgagatgg ccaaagttga cgattctttc tttcaccgtt tggaagagtc
     1861 cttccttgtc gaagaggaca agaaacatga acggcacccc atctttggaa acatagtaga
     1921 tgaggtggca tatcatgaaa agtacccaac gatttatcac ctcagaaaaa agctagttga
     1981 ctcaactgat aaagcggacc tgaggttaat ctacttggct cttgcccata tgataaagtt
     2041 ccgtgggcac tttctcattg agggtgatct aaatccggac aactcggatg tcgacaaact
     2101 gttcatccag ttagtacaaa cctataatca gttgtttgaa gagaacccta taaatgcaag
     2161 tggcgtggat gcgaaggcta ttcttagcgc ccgcctctct aaatcccgac ggctagaaaa
     2221 cctgatcgca caattacccg gagagaagaa aaatgggttg ttcggtaacc ttatagcgct
     2281 ctcactaggc ctgacaccaa attttaagtc gaacttcgac ttagctgaag atgccaaatt
     2341 gcagcttagt aaggacacgt acgatgacga tctcgacaat ctactggcac aaattggaga
     2401 tcagtatgcg gacttatttt tggctgccaa aaaccttagc gatgcaatcc tcctatctga
     2461 catactgaga gttaatactg agattaccaa ggcgccgtta tccgcttcaa tgatcaaaag
     2521 gtacgatgaa catcaccaag acttgacact tctcaaggcc ctagtccgtc agcaactgcc
     2581 tgagaaatat aaggaaatat tctttgatca gtcgaaaaac gggtacgcag gttatattga
     2641 cggcggagcg agtcaagagg aattctacaa gtttatcaaa cccatattag agaagatgga
     2701 tgggacggaa gagttgcttg taaaactcaa tcgcgaagat ctactgcgaa agcagcggac
     2761 tttcgacaac ggtagcattc cacatcaaat ccacttaggc gaattgcatg ctatacttag
     2821 aaggcaggag gatttttatc cgttcctcaa agacaatcgt gaaaagattg agaaaatcct
     2881 aacctttcgc ataccttact atgtgggacc cctggcccga gggaactctc ggttcgcatg
     2941 gatgacaaga aagtccgaag aaacgattac tccatggaat tttgaggaag ttgtcgataa
     3001 aggtgcgtca gctcaatcgt tcatcgagag gatgaccaac tttgacaaga atttaccgaa
     3061 cgaaaaagta ttgcctaagc acagtttact ttacgagtat ttcacagtgt acaatgaact
     3121 cacgaaagtt aagtatgtca ctgagggcat gcgtaaaccc gcctttctaa gcggagaaca
     3181 gaagaaagca atagtagatc tgttattcaa gaccaaccgc aaagtgacag ttaagcaatt
     3241 gaaagaggac tactttaaga aaattgaatg cttcgattct gtcgagatct ccggggtaga
     3301 agatcgattt aatgcgtcac ttggtacgta tcatgacctc ctaaagataa ttaaagataa
     3361 ggacttcctg gataacgaag agaatgaaga tatcttagaa gatatagtgt tgactcttac
     3421 cctctttgaa gatcgggaaa tgattgagga aagactaaaa acatacgctc acctgttcga
     3481 cgataaggtt atgaaacagt taaagaggcg tcgctatacg ggctggggac gattgtcgcg
     3541 gaaacttatc aacgggataa gagacaagca aagtggtaaa actattctcg attttctaaa
     3601 gagcgacggc ttcgccaata ggaactttat gcagctgatc catgatgact ctttaacctt
     3661 caaagaggat atacaaaagg cacaggtttc cggacaaggg gactcattgc acgaacatat
     3721 tgcgaatctt gctggttcgc cagccatcaa aaagggcata ctccagacag tcaaagtagt
     3781 ggatgagcta gttaaggtca tgggacgtca caaaccggaa aacattgtaa tcgagatggc
     3841 acgcgaaaat caaacgactc agaaggggca aaaaaacagt cgagagcgga tgaagagaat
     3901 agaagagggt attaaagaac tgggcagcca gatcttaaag gagcatcctg tggaaaatac
     3961 ccaattgcag aacgagaaac tttacctcta ttacctacaa aatggaaggg acatgtatgt
     4021 tgatcaggaa ctggacataa accgtttatc tgattacgac gtcgatcaca ttgtacccca
     4081 atcctttttg aaggacgatt caatcgacaa taaagtgctt acacgctcgg ataagaaccg
     4141 agggaaaagt gacaatgttc caagcgagga agtcgtaaag aaaatgaaga actattggcg
     4201 gcagctccta aatgcgaaac tgataacgca aagaaagttc gataacttaa ctaaagctga
     4261 gaggggtggc ttgtctgaac ttgacaaggc cggatttatt aaacgtcagc tcgtggaaac
     4321 ccgccaaatc acaaagcatg ttgcacagat actagattcc cgaatgaata cgaaatacga
     4381 cgagaacgat aagctgattc gggaagtcaa agtaatcact ttaaagtcaa aattggtgtc
     4441 ggacttcaga aaggattttc aattctataa agttagggag ataaataact accaccatgc
     4501 gcacgacgct tatcttaatg ccgtcgtagg gaccgcactc attaagaaat acccgaagct
     4561 agaaagtgag tttgtgtatg gtgattacaa agtttatgac gtccgtaaga tgatcgcgaa
     4621 aagcgaacag gagataggca aggctacagc caaatacttc ttttattcta acattatgaa
     4681 tttctttaag acggaaatca ctctggcaaa cggagagata cgcaaacgac ctttaattga
     4741 aaccaatggg gagacaggtg aaatcgtatg ggataagggc cgggacttcg cgacggtgag
     4801 aaaagttttg tccatgcccc aagtcaacat agtaaagaaa actgaggtgc agaccggagg
     4861 gttttcaaag gaatcgattc ttccaaaaag gaatagtgat aagctcatcg ctcgtaaaaa
     4921 ggactgggac ccgaaaaagt acggtggctt cgatagccct acagttgcct attctgtcct
     4981 agtagtggca aaagttgaga agggaaaatc caagaaactg aagtcagtca aagaattatt
     5041 ggggataacg attatggagc gctcgtcttt tgaaaagaac cccatcgact tccttgaggc
     5101 gaaaggttac aaggaagtaa aaaaggatct cataattaaa ctaccaaagt atagtctgtt
     5161 tgagttagaa aatggccgaa aacggatgtt ggctagcgcc ggagagcttc aaaaggggaa
     5221 cgaactcgca ctaccgtcta aatacgtgaa tttcctgtat ttagcgtccc attacgagaa
     5281 gttgaaaggt tcacctgaag ataacgaaca gaagcaactt tttgttgagc agcacaaaca
     5341 ttatctcgac gaaatcatag agcaaatttc ggaattcagt aagagagtca tcctagctga
     5401 tgccaatctg gacaaagtat taagcgcata caacaagcac agggataaac ccatacgtga
     5461 gcaggcggaa aatattatcc atttgtttac tcttaccaac ctcggcgctc cagccgcatt
     5521 caagtatttt gacacaacga tagatcgcaa acgatacact tctaccaagg aggtgctaga
     5581 cgcgacactg attcaccaat ccatcacggg attatatgaa actcggatag atttgtcaca
     5641 gcttgggggt gactctggtg gttctactaa tctgtcagat attattgaaa aggagaccgg
     5701 taagcaactg gttatccagg aatccatcct catgctccca gaggaggtgg aagaagtcat
     5761 tgggaacaag ccggaaagcg atatactcgt gcacaccgcc tacgacgaga gcaccgacga
     5821 gaatgtcatg cttctgacta gcgacgcccc tgaatacaag ccttgggctc tggtcataca
     5881 ggatagcaac ggtgagaaca agattaagat gctctctggt ggttctccca agaagaagag
     5941 gaaagtctaa ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     6001 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6061 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6121 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6181 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6241 agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6301 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6361 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6421 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6481 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6541 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6601 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6661 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6721 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6781 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6841 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6901 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6961 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7021 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7081 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7141 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7201 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7261 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7321 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7381 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7441 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7501 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7561 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7621 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7681 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7741 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7801 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7861 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7921 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7981 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8041 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8101 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8161 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8221 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8281 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8341 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8401 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     8461 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     8521 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     8581 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     8641 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     8701 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     8761 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     8821 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     8881 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     8941 c
//
