LOCUS       sequence-232320-        5388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shuttle vector for baculovirus production, using FlashBac bacmid; 
            for recombinant protein with C-terminal sortase site (LPETGg) and 
            HIS6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Lemaitre RP, Bogdanova A, Borgonovo B, Woodruff JB, Drechsel DN
  TITLE     FlexiBAC: a versatile, open-source baculovirus vector system for 
            protein expression, secretion, and proteolytic processing.
  JOURNAL   BMC Biotechnol. 2019 Mar 29;19(1):20. doi: 
            10.1186/s12896-019-0512-z.
  PUBMED    30925874
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2019-03-29"; volume: "19(1):20. doi"; pages: " 
            10.1186/s12896-019-0512-z"
COMMENT     Sequence Label: pOCC111
FEATURES             Location/Qualifiers
     source          1..5388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             251..556
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     435..454
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             618..635
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    777..911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(814..833)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     868..887
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     3'UTR           984..1356
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     primer_bind     complement(1708..1725)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1818..2406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1898..1917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2577..3368,3369..3437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3200..3219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3438..3542)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3843..3865
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3857..3874
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3858..3874
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3885..3904
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3885..3903
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4514..4638
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(4676..5281)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcgccacca tggcggccgc aagcgctatg gccacaacct gttacattgc
      181 acaagataaa aatatatcat cacgaacagt aaaactgtct gcttacataa acagtaatac
      241 aaggggtgtt atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
      301 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
      361 cagtgcccgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
      421 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
      481 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
      541 gttctgggga atataaatgt caggctccct tatacacagc cagtggcgcg ccgctgcccg
      601 agaccggcgg cagcagccat caccatcacc atcacagtgg ataatagaag cttgtcgaga
      661 agtactagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      901 atgtctggat ctgatcactg cttgagccta ggagatccga accagataag tgaaagcctg
      961 catccgcaga tcttaattaa taaaacacga tacattgtta ttagtacatt tattaagcgc
     1021 tagattctgt gcgttgttga tttacagaca attgttgtac gtattttaat aattcattaa
     1081 atttataatc tttagggtgg tatgttagag cgaaaatcaa atgattttca gcgtctttat
     1141 atctgaattt aaatattaaa tcctcaatag atttgtaaaa taggtttcga ttagtttcaa
     1201 acaagggttg tttttccgaa ccgatggctg gactatctaa tggattttcg ctcaacgcca
     1261 caaaacttgc caaatcttgt agcagcaatc tagctttgtc gatattcgtt tgtgttttgt
     1321 tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac
     1381 tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa taacgcttgg acatatttaa
     1441 catcgggcgt gttagcttta ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag
     1501 aagttgcttc cgaagacgat tttgccatag ccacacgacg cctattaatt gtgtcggcta
     1561 acacgtccgc gatcaaattt gtagttgagc tttttgggcc tcatgggcct tccgctcact
     1621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc atagctgttt
     1681 ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1741 ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2701 gctgcaatga taccgcgaga accacgctca ccggctccag atttatcagc aataaaccag
     2761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3541 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3601 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3661 cttataaatc aaaagaatag accgagatag ggttgagtgg ccgctacagg gcgctcccat
     3721 tcgccattca ggctgcgcaa ctgttgggaa gggcgtttcg gtgcgggcct cttcgctatt
     3781 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3841 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gacgtaatac gactcactat
     3901 agggcgaatt ggcggaaggc cgtcaaggcc gtgacgttaa aactattaag ccatccaatc
     3961 gaccgttagt cgaatcagga ccgctggtgc gagaagccgc gaagtatggc gaatgcatcg
     4021 tataacgtgt ggagtccgct cattagagcg tcatgtttag acaagaaagc tacatattta
     4081 attgatcccg atgattttat tgataaattg accctaactc catacacggt attctacaat
     4141 ggcggggttt tggtcaaaat ttccggactg cgattgtaca tgctgttaac ggctccgccc
     4201 actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa catttgtatg
     4261 aaagaatgcg tagaaggaaa gaaaaatgtc gtggacatgc tgaacaacaa gattaatatg
     4321 cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc gcgcggcggt
     4381 atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc gtgtgccaag
     4441 tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga ctccaagtgt
     4501 gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc accaaactgc
     4561 caaaaaatga aaactgtgga caagctctgt ccgtttgctg gcaactgcaa gggtctcaat
     4621 cctatttgta attattgaat aataaaacaa ttataaatgc taaatttgtt ttttattaac
     4681 gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa aacgcgtagt
     4741 tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta atttgcgaca
     4801 atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg tattccttct
     4861 ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca tatatgtatc
     4921 tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta atggggtgta
     4981 tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct ggtagttctt
     5041 cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga tcgtcggttt
     5101 tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca ccatttttta
     5161 gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac aaaaacagtt
     5221 cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa ataacagcca
     5281 ttgtaatgag acgcacaaac taatatcaca agctgccacc attttgttcg cccaggactc
     5341 tagctatagt tctagtggtt ggctacgtat actccggaat attaatag
//
