LOCUS       sequence-232321-        8974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base editor made from APOBEC3G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris 
            RS
  TITLE     A panel of eGFP reporters for single base editing by APOBEC-Cas9 
            editosome complexes.
  JOURNAL   Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
  PUBMED    30679582
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-01-24"; volume: "9(1):497. doi"; pages: " 
            10.1038/s41598-018-36739-9"
COMMENT     Sequence Label: A3G-Cas9n-UGI
FEATURES             Location/Qualifiers
     source          1..8974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(1586..4984,4985..4987,4988..5584,5585,5586..5588,
                     5589..5590,5591..5593,5594..5686)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1586..1609,1610..1612,1613..5686)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5699..5947
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5960..5980
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5989..6006
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6029..6046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6035..6259
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6330..6346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6330..6346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6343..6365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6354..6370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6378..6384,6385..6402,6403..6408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6423..6444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6561..6578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6732..7320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6812..6831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7491..8282,8283..8351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8114..8133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8352..8456)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8535..8554)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8726..8974,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgaa gcctcacttc agaaacacag tggagcgaat
      421 gtatcgagac acattctcct acaactttta taatagaccc atcctttctc gtcggaatac
      481 cgtctggctg tgctacgaag tgaaaacaaa gggtccctca aggccccctt tggacgcaaa
      541 gatctttcga ggccaggtgt attccgaact taagtaccac ccagagatga gattcttcca
      601 ctggttcagc aagtggagga agctgcatcg tgaccaggag tatgaggtca cctggtacat
      661 atcctggagc ccctgcacaa agtgtacaag ggatatggcc acgttcctgg ccgaggaccc
      721 gaaggttacc ctgaccatct ttgttgcccg cctctactac ttctgggacc cagattacca
      781 ggaggcgctt cgcagcctgt gtcagaaaag agacggtccg cgtgccacca tgaagatcat
      841 gaattatgac gaatttcagc actgttggag caagttcgtg tacagccaaa gagagctatt
      901 tgagccttgg aataatctgc ctaaatatta tatattactg cacatcatgc tgggggagat
      961 tctcagacac tcgatggatc cacccacatt cactttcaac tttaacaatg aaccttgggt
     1021 cagaggacgg catgagactt acctgtgtta tgaggtggag cgcatgcaca atgacacctg
     1081 ggtcctgctg aaccagcgca ggggctttct atgcaaccag gctccacata aacacggttt
     1141 ccttgaaggc cgccatgcag agctgtgctt cctggacgtg attccctttt ggaagctgga
     1201 cctggaccag gactacaggg ttacctgctt cacctcctgg agcccctgct tcagctgtgc
     1261 ccaggaaatg gctaaattca tttcaaaaaa caaacacgtg agcctgtgca tcttcactgc
     1321 ccgcatctat gatgatcaag gaagatgtca ggaggggctg cgcaccctgg ccgaggctgg
     1381 ggccaaaatt tcaataatga catacagtga atttaagcac tgctgggaca cctttgtgga
     1441 ccaccaggga tgtcccttcc agccctggga tggactagat gagcacagcc aagacctgag
     1501 tgggaggctg cgggccattc tccagaatca ggaaaacagc ggcagcgaga ctcccgggac
     1561 ctcagagtcc gccacacccg aaagtgataa aaagtattct attggtttag ccatcggcac
     1621 taattccgtt ggatgggctg tcataaccga tgaatacaaa gtaccttcaa agaaatttaa
     1681 ggtgttgggg aacacagacc gtcattcgat taaaaagaat cttatcggtg ccctcctatt
     1741 cgatagtggc gaaacggcag aggcgactcg cctgaaacga accgctcgga gaaggtatac
     1801 acgtcgcaag aaccgaatat gttacttaca agaaattttt agcaatgaga tggccaaagt
     1861 tgacgattct ttctttcacc gtttggaaga gtccttcctt gtcgaagagg acaagaaaca
     1921 tgaacggcac cccatctttg gaaacatagt agatgaggtg gcatatcatg aaaagtaccc
     1981 aacgatttat cacctcagaa aaaagctagt tgactcaact gataaagcgg acctgaggtt
     2041 aatctacttg gctcttgccc atatgataaa gttccgtggg cactttctca ttgagggtga
     2101 tctaaatccg gacaactcgg atgtcgacaa actgttcatc cagttagtac aaacctataa
     2161 tcagttgttt gaagagaacc ctataaatgc aagtggcgtg gatgcgaagg ctattcttag
     2221 cgcccgcctc tctaaatccc gacggctaga aaacctgatc gcacaattac ccggagagaa
     2281 gaaaaatggg ttgttcggta accttatagc gctctcacta ggcctgacac caaattttaa
     2341 gtcgaacttc gacttagctg aagatgccaa attgcagctt agtaaggaca cgtacgatga
     2401 cgatctcgac aatctactgg cacaaattgg agatcagtat gcggacttat ttttggctgc
     2461 caaaaacctt agcgatgcaa tcctcctatc tgacatactg agagttaata ctgagattac
     2521 caaggcgccg ttatccgctt caatgatcaa aaggtacgat gaacatcacc aagacttgac
     2581 acttctcaag gccctagtcc gtcagcaact gcctgagaaa tataaggaaa tattctttga
     2641 tcagtcgaaa aacgggtacg caggttatat tgacggcgga gcgagtcaag aggaattcta
     2701 caagtttatc aaacccatat tagagaagat ggatgggacg gaagagttgc ttgtaaaact
     2761 caatcgcgaa gatctactgc gaaagcagcg gactttcgac aacggtagca ttccacatca
     2821 aatccactta ggcgaattgc atgctatact tagaaggcag gaggattttt atccgttcct
     2881 caaagacaat cgtgaaaaga ttgagaaaat cctaaccttt cgcatacctt actatgtggg
     2941 acccctggcc cgagggaact ctcggttcgc atggatgaca agaaagtccg aagaaacgat
     3001 tactccatgg aattttgagg aagttgtcga taaaggtgcg tcagctcaat cgttcatcga
     3061 gaggatgacc aactttgaca agaatttacc gaacgaaaaa gtattgccta agcacagttt
     3121 actttacgag tatttcacag tgtacaatga actcacgaaa gttaagtatg tcactgaggg
     3181 catgcgtaaa cccgcctttc taagcggaga acagaagaaa gcaatagtag atctgttatt
     3241 caagaccaac cgcaaagtga cagttaagca attgaaagag gactacttta agaaaattga
     3301 atgcttcgat tctgtcgaga tctccggggt agaagatcga tttaatgcgt cacttggtac
     3361 gtatcatgac ctcctaaaga taattaaaga taaggacttc ctggataacg aagagaatga
     3421 agatatctta gaagatatag tgttgactct taccctcttt gaagatcggg aaatgattga
     3481 ggaaagacta aaaacatacg ctcacctgtt cgacgataag gttatgaaac agttaaagag
     3541 gcgtcgctat acgggctggg gacgattgtc gcggaaactt atcaacggga taagagacaa
     3601 gcaaagtggt aaaactattc tcgattttct aaagagcgac ggcttcgcca ataggaactt
     3661 tatgcagctg atccatgatg actctttaac cttcaaagag gatatacaaa aggcacaggt
     3721 ttccggacaa ggggactcat tgcacgaaca tattgcgaat cttgctggtt cgccagccat
     3781 caaaaagggc atactccaga cagtcaaagt agtggatgag ctagttaagg tcatgggacg
     3841 tcacaaaccg gaaaacattg taatcgagat ggcacgcgaa aatcaaacga ctcagaaggg
     3901 gcaaaaaaac agtcgagagc ggatgaagag aatagaagag ggtattaaag aactgggcag
     3961 ccagatctta aaggagcatc ctgtggaaaa tacccaattg cagaacgaga aactttacct
     4021 ctattaccta caaaatggaa gggacatgta tgttgatcag gaactggaca taaaccgttt
     4081 atctgattac gacgtcgatc acattgtacc ccaatccttt ttgaaggacg attcaatcga
     4141 caataaagtg cttacacgct cggataagaa ccgagggaaa agtgacaatg ttccaagcga
     4201 ggaagtcgta aagaaaatga agaactattg gcggcagctc ctaaatgcga aactgataac
     4261 gcaaagaaag ttcgataact taactaaagc tgagaggggt ggcttgtctg aacttgacaa
     4321 ggccggattt attaaacgtc agctcgtgga aacccgccaa atcacaaagc atgttgcaca
     4381 gatactagat tcccgaatga atacgaaata cgacgagaac gataagctga ttcgggaagt
     4441 caaagtaatc actttaaagt caaaattggt gtcggacttc agaaaggatt ttcaattcta
     4501 taaagttagg gagataaata actaccacca tgcgcacgac gcttatctta atgccgtcgt
     4561 agggaccgca ctcattaaga aatacccgaa gctagaaagt gagtttgtgt atggtgatta
     4621 caaagtttat gacgtccgta agatgatcgc gaaaagcgaa caggagatag gcaaggctac
     4681 agccaaatac ttcttttatt ctaacattat gaatttcttt aagacggaaa tcactctggc
     4741 aaacggagag atacgcaaac gacctttaat tgaaaccaat ggggagacag gtgaaatcgt
     4801 atgggataag ggccgggact tcgcgacggt gagaaaagtt ttgtccatgc cccaagtcaa
     4861 catagtaaag aaaactgagg tgcagaccgg agggttttca aaggaatcga ttcttccaaa
     4921 aaggaatagt gataagctca tcgctcgtaa aaaggactgg gacccgaaaa agtacggtgg
     4981 cttcgatagc cctacagttg cctattctgt cctagtagtg gcaaaagttg agaagggaaa
     5041 atccaagaaa ctgaagtcag tcaaagaatt attggggata acgattatgg agcgctcgtc
     5101 ttttgaaaag aaccccatcg acttccttga ggcgaaaggt tacaaggaag taaaaaagga
     5161 tctcataatt aaactaccaa agtatagtct gtttgagtta gaaaatggcc gaaaacggat
     5221 gttggctagc gccggagagc ttcaaaaggg gaacgaactc gcactaccgt ctaaatacgt
     5281 gaatttcctg tatttagcgt cccattacga gaagttgaaa ggttcacctg aagataacga
     5341 acagaagcaa ctttttgttg agcagcacaa acattatctc gacgaaatca tagagcaaat
     5401 ttcggaattc agtaagagag tcatcctagc tgatgccaat ctggacaaag tattaagcgc
     5461 atacaacaag cacagggata aacccatacg tgagcaggcg gaaaatatta tccatttgtt
     5521 tactcttacc aacctcggcg ctccagccgc attcaagtat tttgacacaa cgatagatcg
     5581 caaacgatac acttctacca aggaggtgct agacgcgaca ctgattcacc aatccatcac
     5641 gggattatat gaaactcgga tagatttgtc acagcttggg ggtgactctg gtggttctac
     5701 taatctgtca gatattattg aaaaggagac cggtaagcaa ctggttatcc aggaatccat
     5761 cctcatgctc ccagaggagg tggaagaagt cattgggaac aagccggaaa gcgatatact
     5821 cgtgcacacc gcctacgacg agagcaccga cgagaatgtc atgcttctga ctagcgacgc
     5881 ccctgaatac aagccttggg ctctggtcat acaggatagc aacggtgaga acaagattaa
     5941 gatgctctct ggtggttctc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat
     6001 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6061 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6121 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6181 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6241 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg
     6301 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6361 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc
     6421 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     6481 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     6541 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     6601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7141 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7441 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     7501 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     7561 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     7621 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7681 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7741 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7801 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7861 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7921 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7981 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8041 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8101 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8161 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8221 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8281 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8341 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8401 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     8461 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta
     8521 gggtcgactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8581 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     8641 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     8701 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     8761 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8821 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8881 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8941 taaactgccc acttggcagt acatcaagtg tatc
//
