LOCUS       sequence-232336-        8371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base editor made from APOBEC3H Haplotype I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris 
            RS
  TITLE     A panel of eGFP reporters for single base editing by APOBEC-Cas9 
            editosome complexes.
  JOURNAL   Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
  PUBMED    30679582
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-01-24"; volume: "9(1):497. doi"; pages: " 
            10.1038/s41598-018-36739-9"
COMMENT     Sequence Label: A3H HapI-Cas9n-UGI
FEATURES             Location/Qualifiers
     source          1..8371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      380..389
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(983..4381,4382..4384,4385..4981,4982,4983..4985,
                     4986..4987,4988..4990,4991..5083)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(983..1006,1007..1009,1010..5083)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5096..5344
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5357..5377
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5386..5403
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5426..5443)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5432..5656
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5727..5743)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5727..5743)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5740..5762)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5751..5767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5775..5781,5782..5799,5800..5805))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5820..5841
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5958..5975)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6129..6717)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6209..6228)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6888..7679,7680..7748))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7511..7530
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7749..7853)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7932..7951)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8123..8371,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatggc tctgttaaca gccgaaacat tccgcttaca
      421 gtttaacaac aagcgccgcc tcagaaggcc ttactacccg aggaaggccc tcttgtgtta
      481 ccagctgacg ccgcagaatg gctccacgcc cacgagaggc tactttgaaa acaagaaaaa
      541 gtgccatgca gaaatttgct ttattaacga gatcaagtcc atgggactgg acgaaacgca
      601 gtgctaccaa gtcacctgtt acctcacgtg gagcccctgc tcctcctgtg cctgggagct
      661 ggttgacttc atcaaggctc acgaccatct gaacctgggc atcttcgcct cccgcctgta
      721 ctaccactgg tgcaagcccc agcagaaggg gctgcggctt ctgtgtggat cccaggtccc
      781 ggtggaggtc atgggcttcc cagagtttgc tgactgctgg gaaaactttg tggaccacga
      841 gaaaccgctt tccttcaacc cctataagat gttagaggag ctagataaaa acagtcgagc
      901 cataaagcga cggcttgaga ggataaagca gtccagcggc agcgagactc ccgggacctc
      961 agagtccgcc acacccgaaa gtgataaaaa gtattctatt ggtttagcca tcggcactaa
     1021 ttccgttgga tgggctgtca taaccgatga atacaaagta ccttcaaaga aatttaaggt
     1081 gttggggaac acagaccgtc attcgattaa aaagaatctt atcggtgccc tcctattcga
     1141 tagtggcgaa acggcagagg cgactcgcct gaaacgaacc gctcggagaa ggtatacacg
     1201 tcgcaagaac cgaatatgtt acttacaaga aatttttagc aatgagatgg ccaaagttga
     1261 cgattctttc tttcaccgtt tggaagagtc cttccttgtc gaagaggaca agaaacatga
     1321 acggcacccc atctttggaa acatagtaga tgaggtggca tatcatgaaa agtacccaac
     1381 gatttatcac ctcagaaaaa agctagttga ctcaactgat aaagcggacc tgaggttaat
     1441 ctacttggct cttgcccata tgataaagtt ccgtgggcac tttctcattg agggtgatct
     1501 aaatccggac aactcggatg tcgacaaact gttcatccag ttagtacaaa cctataatca
     1561 gttgtttgaa gagaacccta taaatgcaag tggcgtggat gcgaaggcta ttcttagcgc
     1621 ccgcctctct aaatcccgac ggctagaaaa cctgatcgca caattacccg gagagaagaa
     1681 aaatgggttg ttcggtaacc ttatagcgct ctcactaggc ctgacaccaa attttaagtc
     1741 gaacttcgac ttagctgaag atgccaaatt gcagcttagt aaggacacgt acgatgacga
     1801 tctcgacaat ctactggcac aaattggaga tcagtatgcg gacttatttt tggctgccaa
     1861 aaaccttagc gatgcaatcc tcctatctga catactgaga gttaatactg agattaccaa
     1921 ggcgccgtta tccgcttcaa tgatcaaaag gtacgatgaa catcaccaag acttgacact
     1981 tctcaaggcc ctagtccgtc agcaactgcc tgagaaatat aaggaaatat tctttgatca
     2041 gtcgaaaaac gggtacgcag gttatattga cggcggagcg agtcaagagg aattctacaa
     2101 gtttatcaaa cccatattag agaagatgga tgggacggaa gagttgcttg taaaactcaa
     2161 tcgcgaagat ctactgcgaa agcagcggac tttcgacaac ggtagcattc cacatcaaat
     2221 ccacttaggc gaattgcatg ctatacttag aaggcaggag gatttttatc cgttcctcaa
     2281 agacaatcgt gaaaagattg agaaaatcct aacctttcgc ataccttact atgtgggacc
     2341 cctggcccga gggaactctc ggttcgcatg gatgacaaga aagtccgaag aaacgattac
     2401 tccatggaat tttgaggaag ttgtcgataa aggtgcgtca gctcaatcgt tcatcgagag
     2461 gatgaccaac tttgacaaga atttaccgaa cgaaaaagta ttgcctaagc acagtttact
     2521 ttacgagtat ttcacagtgt acaatgaact cacgaaagtt aagtatgtca ctgagggcat
     2581 gcgtaaaccc gcctttctaa gcggagaaca gaagaaagca atagtagatc tgttattcaa
     2641 gaccaaccgc aaagtgacag ttaagcaatt gaaagaggac tactttaaga aaattgaatg
     2701 cttcgattct gtcgagatct ccggggtaga agatcgattt aatgcgtcac ttggtacgta
     2761 tcatgacctc ctaaagataa ttaaagataa ggacttcctg gataacgaag agaatgaaga
     2821 tatcttagaa gatatagtgt tgactcttac cctctttgaa gatcgggaaa tgattgagga
     2881 aagactaaaa acatacgctc acctgttcga cgataaggtt atgaaacagt taaagaggcg
     2941 tcgctatacg ggctggggac gattgtcgcg gaaacttatc aacgggataa gagacaagca
     3001 aagtggtaaa actattctcg attttctaaa gagcgacggc ttcgccaata ggaactttat
     3061 gcagctgatc catgatgact ctttaacctt caaagaggat atacaaaagg cacaggtttc
     3121 cggacaaggg gactcattgc acgaacatat tgcgaatctt gctggttcgc cagccatcaa
     3181 aaagggcata ctccagacag tcaaagtagt ggatgagcta gttaaggtca tgggacgtca
     3241 caaaccggaa aacattgtaa tcgagatggc acgcgaaaat caaacgactc agaaggggca
     3301 aaaaaacagt cgagagcgga tgaagagaat agaagagggt attaaagaac tgggcagcca
     3361 gatcttaaag gagcatcctg tggaaaatac ccaattgcag aacgagaaac tttacctcta
     3421 ttacctacaa aatggaaggg acatgtatgt tgatcaggaa ctggacataa accgtttatc
     3481 tgattacgac gtcgatcaca ttgtacccca atcctttttg aaggacgatt caatcgacaa
     3541 taaagtgctt acacgctcgg ataagaaccg agggaaaagt gacaatgttc caagcgagga
     3601 agtcgtaaag aaaatgaaga actattggcg gcagctccta aatgcgaaac tgataacgca
     3661 aagaaagttc gataacttaa ctaaagctga gaggggtggc ttgtctgaac ttgacaaggc
     3721 cggatttatt aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg ttgcacagat
     3781 actagattcc cgaatgaata cgaaatacga cgagaacgat aagctgattc gggaagtcaa
     3841 agtaatcact ttaaagtcaa aattggtgtc ggacttcaga aaggattttc aattctataa
     3901 agttagggag ataaataact accaccatgc gcacgacgct tatcttaatg ccgtcgtagg
     3961 gaccgcactc attaagaaat acccgaagct agaaagtgag tttgtgtatg gtgattacaa
     4021 agtttatgac gtccgtaaga tgatcgcgaa aagcgaacag gagataggca aggctacagc
     4081 caaatacttc ttttattcta acattatgaa tttctttaag acggaaatca ctctggcaaa
     4141 cggagagata cgcaaacgac ctttaattga aaccaatggg gagacaggtg aaatcgtatg
     4201 ggataagggc cgggacttcg cgacggtgag aaaagttttg tccatgcccc aagtcaacat
     4261 agtaaagaaa actgaggtgc agaccggagg gttttcaaag gaatcgattc ttccaaaaag
     4321 gaatagtgat aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt acggtggctt
     4381 cgatagccct acagttgcct attctgtcct agtagtggca aaagttgaga agggaaaatc
     4441 caagaaactg aagtcagtca aagaattatt ggggataacg attatggagc gctcgtcttt
     4501 tgaaaagaac cccatcgact tccttgaggc gaaaggttac aaggaagtaa aaaaggatct
     4561 cataattaaa ctaccaaagt atagtctgtt tgagttagaa aatggccgaa aacggatgtt
     4621 ggctagcgcc ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta aatacgtgaa
     4681 tttcctgtat ttagcgtccc attacgagaa gttgaaaggt tcacctgaag ataacgaaca
     4741 gaagcaactt tttgttgagc agcacaaaca ttatctcgac gaaatcatag agcaaatttc
     4801 ggaattcagt aagagagtca tcctagctga tgccaatctg gacaaagtat taagcgcata
     4861 caacaagcac agggataaac ccatacgtga gcaggcggaa aatattatcc atttgtttac
     4921 tcttaccaac ctcggcgctc cagccgcatt caagtatttt gacacaacga tagatcgcaa
     4981 acgatacact tctaccaagg aggtgctaga cgcgacactg attcaccaat ccatcacggg
     5041 attatatgaa actcggatag atttgtcaca gcttgggggt gactctggtg gttctactaa
     5101 tctgtcagat attattgaaa aggagaccgg taagcaactg gttatccagg aatccatcct
     5161 catgctccca gaggaggtgg aagaagtcat tgggaacaag ccggaaagcg atatactcgt
     5221 gcacaccgcc tacgacgaga gcaccgacga gaatgtcatg cttctgacta gcgacgcccc
     5281 tgaatacaag ccttgggctc tggtcataca ggatagcaac ggtgagaaca agattaagat
     5341 gctctctggt ggttctccca agaagaagag gaaagtctaa ccggtcatca tcaccatcac
     5401 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5461 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5521 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5581 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5641 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc
     5701 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5761 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa
     5821 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5881 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5941 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6001 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6061 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6121 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6181 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6241 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6301 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6361 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6421 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6481 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6541 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6601 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6661 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6721 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6781 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6841 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6901 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6961 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7021 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7081 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7141 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7201 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7261 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7321 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7381 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7441 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7501 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7561 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7621 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7681 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7741 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7801 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7861 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg
     7921 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     7981 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     8041 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     8101 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     8161 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     8221 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     8281 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     8341 actgcccact tggcagtaca tcaagtgtat c
//
