LOCUS       sequence-232339-        4235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mClover labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-proD/mClover/nat
FEATURES             Location/Qualifiers
     source          1..4235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    2161..2179
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(2245..2262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2416..3004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2496..2515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3175..3966,3967..4035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3798..3817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4036..4140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4208..4226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgaattcc acagctaaca ccacgtcgtc cctatctgct gccctaggtc
      421 tatgagtggt tgctggataa ctttacgggc atgcataagg ctcgtataat atattcaggg
      481 agaccacaac ggtttccctc tacaaataat tttgtttaac tttgaattct aaggaggaaa
      541 aaaaatggtg agtaaaggcg aagaactgtt taccggcgta gtaccaattc ttgttgaact
      601 tgatggcgac gtgaatggtc acaaattctc ggtccgcggt gaaggcgagg gtgacgcaac
      661 caacggcaag ctgacgctga aatttatttg caccaccggt aaactgccgg ttccgtggcc
      721 tacgctcgtg accacgtttg gttatggtgt agcatgcttt tcccgttacc cggaccacat
      781 gaagcagcat gactttttta aatccgctat gccggaaggt tatgttcagg aacgcaccat
      841 cagctttaaa gacgacggta cgtacaaaac gcgcgccgaa gtgaaatttg aaggtgatac
      901 cctcgttaac cgcattgagc tgaagggcat tgatttcaaa gaagatggta atatccttgg
      961 tcataaactg gaatacaatt tcaattcgca caacgtgtat attacggccg ataagcagaa
     1021 aaatggtatt aaggcaaatt tcaaaatccg tcataacgtg gaagacggtt ccgtccagct
     1081 tgctgatcat taccagcaga acacgccgat tggcgatggt ccggtactgc tgcctgataa
     1141 ccactacctt tctcaccagt cagcactttc caaagatccg aacgaaaaac gcgatcatat
     1201 ggtactgctc gagtttgtaa cggccgcggg catcacccac ggcatggacg aactttacaa
     1261 ataagtcgac ggaggacagt aatgggtacg acccttgatg acacggctta tcggtatcgc
     1321 acctctgtcc cgggtgacgc cgaagccatc gaagcactgg atgggtcctt caccaccgac
     1381 accgtcttcc gtgtcaccgc aaccggtgac ggcttcaccc tgcgggaggt gccggttgac
     1441 ccgcccctga ccaaggtgtt tccggacgac gaaagcgatg acgaatcgga cgatggtgaa
     1501 gacggtgatc cggactcccg gacgttcgtc gcgtatggtg acgatggcga cctggcaggc
     1561 ttcgtggtcg tcagctatag cggctggaac cgccgtctga ccgtcgaaga catcgaagtc
     1621 gcaccagaac accgtggtca cggtgtcggt cgtgcattga tgggtttagc aacggaattt
     1681 gcccgtgaac gtggtgcagg tcacctctgg ctggaagtca ccaacgtcaa cgcaccggcg
     1741 atccacgcgt atcgtcgtat ggggttcacc ctctgcggcc tggacaccgc cctgtacgac
     1801 ggcacggcca gcgatggcga acaggcgctc tacatgagca tgccctgccc gtaaaagctt
     1861 taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag
     1921 ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga
     1981 gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac
     2041 atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg
     2101 tactatcaac aggttgaact gccaacgact acgcactagc caacaagagc ttcagggttg
     2161 agatgtgtat aagagacagc tgtcttaatg aatcggccaa cgcgcgggga gaggcggttt
     2221 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2281 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2341 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2581 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2821 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     2881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3481 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3781 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4141 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4201 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
