LOCUS       sequence-23234-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: p2GB42/Kan
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..270
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /label=B42 transcriptional activator
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
     CDS             277..303
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      465..630
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(520..544)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctcctccnn aaaagaagag aaaggtagct ggtatcaata aagatatcga ggagtgcaat
       61 gccatcattg agcagtttat cgactacctg cgcaccggac aggagatgcc gatggaaatg
      121 gcggatcagg cgattaacgt ggtgccgggc atgacgccga aaaccattct tcacgccggg
      181 ccgccgatcc agcctgactg gctgaaatcg aatggttttc atgaaattga agcggatgtt
      241 aacgatacca gcctcttgct gagtggagat gcctcctacc cttatgatgt gccagattat
      301 gcctctcccg aattcggccg actcgagaag ctttggactt cttcgccaga ggtttggtca
      361 agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa
      421 agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat
      481 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
      541 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggg
      601 tnnnttctca ggtatagcat gaggtcgctc ttattgacca cacctcta
//
