LOCUS       sequence-232348-        5727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial tdTomato labeling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-proD/tdTomato-nat
FEATURES             Location/Qualifiers
     source          1..5727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     83..102
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    241..259
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     mobile_element  399..1166
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     primer_bind     complement(3669..3686)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3840..4428)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3920..3939)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4599..5390,5391..5459))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5222..5241
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5460..5564)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5632..5650
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5688..5710)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
       61 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
      121 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
      181 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgacag
      241 ctgtctctta tacacatctc aaccatcatc gatgaatttt ctcgggtgtt ctcgcatatt
      301 ggctcgaatt ccacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg
      361 gttgctggat aactttacgg gcatgcataa ggctcgtagg taatgactcc aacttattga
      421 tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac cgattttgag
      481 aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag gttatgccgc
      541 tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag gcgggattca
      601 tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag caggctcata
      661 agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac cgtcttccgg
      721 agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac atagccccac
      781 tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg cgaggttacc
      841 gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg cccataatgc
      901 gggctgttgc ccggcatcca acgccattca tggccatatc aatgattttc tggtgcgtac
      961 cgggttgaga agcggtgtaa gtgaactgca gttgccatgt tttacggcag tgagagcaga
     1021 gatagcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca gtagctgaac
     1081 aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac catcatacac
     1141 taaatcagta agttggcagc atcaccaggc tcgtataata tattcaggga gaccacaacg
     1201 gtttccctct acaaataatt ttgtttaact tttaaagagg aaaaaaaatg gtgagtaagg
     1261 gcgaggaggt cattaaggag ttcatgcggt tcaaggtcag gatggagggc agcatgaacg
     1321 gtcatgagtt cgaaatcgaa ggagaaggag agggccggcc gtacgagggg acgcagaccg
     1381 cgaagctgaa ggtgacgaag ggcggcccac tccccttcgc atgggacatc ctgtcgccgc
     1441 agttcatgta tggttccaag gcgtacgtga agcatccggc ggacataccg gactacaaga
     1501 agctgagctt cccggagggg ttcaagtggg agcgggtcat gaactttgag gacggcggcc
     1561 ttgtcacagt gacccaggat agcagtttgc aggacggcac gctgatctat aaggtgaaga
     1621 tgcgagggac caacttcccg cccgacggcc ccgtgatgca gaagaagacc atgggctggg
     1681 aggcttcgac ggagcgcctg tacccgcgtg acggtgtgct gaagggcgaa atccaccagg
     1741 ccctgaagct gaaggatgga ggtcactacc tcgtcgagtt caagaccatc tacatggcca
     1801 aaaagcccgt gcagctcccc ggctactact atgttgacac caagctcgac attacctcgc
     1861 ataacgagga ctataccatc gtggagcaat acgaacgctc ggagggccgg caccacctgt
     1921 tcctcggtca tgggaccggt tcgactggca gcggtagttc aggaaccgcc tcgtcggagg
     1981 acaacaatat ggccgtgatc aaagagttca tgcgcttcaa ggtgcgaatg gaaggttcga
     2041 tgaacggcca cgagttcgag atcgaggggg aaggcgaagg tcggccatac gaaggcaccc
     2101 agacggcgaa gttgaaagtg accaagggcg gcccgctgcc gttcgcgtgg gacatcctgt
     2161 caccgcaatt catgtatggc tcgaaggcct acgtcaagca cccggccgac attccagact
     2221 acaagaagct ctcttttccc gagggcttca agtgggagcg cgtcatgaac tttgaggatg
     2281 gtggcctggt gaccgtaacg caggacagct ccctccagga cgggaccctg atctataaag
     2341 tgaagatgcg cggcaccaac ttcccacccg atggcccagt aatgcaaaag aaaacgatgg
     2401 gatgggaggc aagcaccgaa cgtctgtacc cgcgcgacgg agtcctgaag ggtgaaatcc
     2461 accaagccct aaaactgaag gacggtgggc actacctggt tgagttcaag accatctata
     2521 tggcaaagaa gcccgtgcaa ctccccggat attactacgt ggacacgaag ctggacatca
     2581 ccagccacaa cgaagactac acgatcgtcg agcaatacga acggagtgag ggtcggcacc
     2641 atctcttcct ctatggtatg gacgagctgt acaagtaagg aggacagtaa tgggtacgac
     2701 ccttgatgac acggcttatc ggtatcgcac ctctgtcccg ggtgacgccg aagccatcga
     2761 agcactggat gggtccttca ccaccgacac cgtcttccgt gtcaccgcaa ccggtgacgg
     2821 cttcaccctg cgggaggtgc cggttgaccc gcccctgacc aaggtgtttc cggacgacga
     2881 aagcgatgac gaatcggacg atggtgaaga cggtgatccg gactcccgga cgttcgtcgc
     2941 gtatggtgac gatggcgacc tggcaggctt cgtggtcgtc agctatagcg gctggaaccg
     3001 ccgtctgacc gtcgaagaca tcgaagtcgc accagaacac cgtggtcacg gtgtcggtcg
     3061 tgcattgatg ggtttagcaa cggaatttgc ccgtgaacgt ggtgcaggtc acctctggct
     3121 ggaagtcacc aacgtcaacg caccggcgat ccacgcgtat cgtcgtatgg ggttcaccct
     3181 ctgcggcctg gacaccgccc tgtacgacgg cacggccagc gatggcgaac aggcgctcta
     3241 catgagcatg ccctgcccgt aagtcgacct gcaggcatgc aagctttaaa agccttatat
     3301 attctttttt ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat
     3361 taattttatt gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag
     3421 ccatgagagc ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta
     3481 cgttaaacat gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt
     3541 tgaactgcca acgactacgc actagccaac aagagcttca gggttgagat gtgtataaga
     3601 gctgtctctt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3901 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3961 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4021 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4081 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4141 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4201 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4261 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4321 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4381 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4441 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4501 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4561 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4621 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4681 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4741 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4801 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4861 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4921 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4981 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5041 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5101 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5161 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5221 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5281 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5341 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5401 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5461 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5521 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5581 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5641 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     5701 cagctcccgg agacggtcac agcttgt
//
