LOCUS       sequence-23235-e         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: pCGB42/Kan
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..258
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /label=B42 transcriptional activator
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(317..333)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(317..333)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(544..568)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 aagaagagaa aggtagctgg tatcaataaa gatatcgagg agtgcaatgc catcattgag
       61 cagtttatcg actacctgcg caccggacag gagatgccga tggaaatggc ggatcaggcg
      121 attaacgtgg tgccgggcat gacgccgaaa accattcttc acgccgggcc gccgatccag
      181 cctgactggc tgaaatcgaa tggttttcat gaaattgaag cggatgttaa cgataccagc
      241 ctcttgctga gtggagatgc ctcctaccct tatgatgtgc cagattatgc ctctcccgaa
      301 ttcctgcagc ccgggggatc cactagttct agagcggccg ccaccgcggt ggagctttgg
      361 acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc
      421 cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt
      481 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
      541 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
      601 gtaactcttt cctgtaggtc aggttgcttt ct
//
