LOCUS       sequence-23236-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: p2GLex
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..267
                     /codon_start=1
                     /gene="lexA (truncated)"
                     /product="DNA-binding domain of E. coli LexA"
                     /label=LexA BD
                     /note="binds the 16-bp palindromic sequence 
                     5'-CTGTATATATATACAG-3'"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
     primer_bind     559..580
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 ttaattccgg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
       61 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
      121 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgtt
      181 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
      241 ttgccgctgg taggtcgtgt ggctgccggt gaaccacttc tggcgcaaca gcatattgaa
      301 ggtcattatc aggtcgatcc ttccttattc aagccgaatg ctgatttcct gctgcgcgtc
      361 agcgggatgt cgatgaaaga tatcggcatt atggatggtg acttgctggc agtgcataaa
      421 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga ggttaccgtt
      481 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
      541 ccaattgtcg tagatcttcg tcagcagagc ttcaccattg aagggcntgg cggttggggt
      601 tattcgcaac ggcgactggn tggaattncc cgcgggg
//
