LOCUS       sequence-232362-        9971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express gag-gfp fusion driven by TK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: ptcALPS gag-gfp Blasti
FEATURES             Location/Qualifiers
     source          1..9971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..84)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        69..398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      249..384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     311..330
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        410..789
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        791..989
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     944..964
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1007..1187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1234..1359
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2273..2317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2615..2732
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2776..3527
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      3534..3543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3538..3547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5058..5060,5061..5063,5064..5774)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      6272..6281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6462..6473
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    6686..7274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6739..6759)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7157..7168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7792..7972
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8043..8631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8123..8142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8802..9593,9594..9662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9425..9444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9663..9767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9842..9861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
       61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
      361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
      421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
     1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
     2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2761 cagtttggtt aattaaatga gtcttcggac ctcgcggggg ccgcttaagc ggtggttagg
     2821 gtttgtctga cgcgggggga gggggaagga acgaaacact ctcattcgga ggcggctcgg
     2881 ggtttggtct tggtggccac gggcacgcag aagagcgccg cgatcctctt aagcaccccc
     2941 ccgccctccg tggaggcggg ggtttggtcg gcgggtggta actggcgggc cgctgactcg
     3001 ggcgggtcgc gcgccccaga gtgtgacctt ttcggtctgc tcgcagaccc ccgggcggcg
     3061 ccgccgcggc ggcgacgggc tcgctgggtc ctaggctcca tggggaccgt atacgtggac
     3121 aggctctgga gcatccgcac gactgcggtg atattaccgg agaccttctg cgggacgagc
     3181 cgggtcacgc ggctgacgcg gagcgtccgt tgggcgacaa acaccaggac ggggcacagg
     3241 tacactatct tgtcacccgg aggcgcgagg gactgcagga gcttcaggga gtggcgcagc
     3301 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     3361 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     3421 tcgaacacgc agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca tattaaggtg
     3481 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaatct agagccgcca
     3541 tggcggcaag ggcaagcgtg ctgagcgggg gggagctgga caaatgggag aaaatccggc
     3601 tgagacctgg aggcaagaaa cagtataaac tgaagcacat cgtgtgggct agcagagaac
     3661 tggagaggtt cgcagtcaac cccggcctgc tggaaacatc cgagggatgc cgacagattc
     3721 tggggcagct gcagccttcc ctgcagactg gatccgagga actgcgcagt ctgtacaaca
     3781 cagtggccac cctgtattgc gtgcatcagc ggattgacgt gaaagatacc aaggaggctc
     3841 tggacaaaat cgaggaagag cagaataagt ccaagaaaaa ggcacagcag gcggccgcag
     3901 atacagggaa caatagccag gtgtcccaga actacccaat cgtccagaat ctgcagggac
     3961 agatggtgca ccaggccatt tctcccagaa ccctgaacgc ttgggtgaaa gtggtcgaag
     4021 agaaggcctt cagtcctgaa gtcatcccaa tgttttctgc tctgagtgag ggcgcaacac
     4081 ctcaagatct aaacacaatg ctgaatactg tgggcgggca tcaggccgct atgcagatgc
     4141 tgaaggaaac tatcaatgaa gaggcagcag agtgggacag gctgcaccca gtgcatgcag
     4201 gaccaattgc acctgggcag atgcgagaac ctagaggatc agatatcgcc ggcaccacaa
     4261 gcacactgca ggagcagatt gggtggatga ctcacaaccc ccctataccg gtaggagaaa
     4321 tctacaaaag gtggatcatt ctgggcctga ataagatcgt ccgcatgtat tcacccacta
     4381 gcatcctgga cattcgacaa ggcccaaaag agcccttccg cgactacgtg gacagattct
     4441 acaagaccct gcgcgccgaa caagcttcac aggaggtgaa aaactggatg actgagaccc
     4501 tgctggtcca gaacgctaat cctgattgca aaacaattct gaaggccctg gggccaggag
     4561 ctactctgga agagatgatg accgcatgtc agggagtggg aggacctgga cacaaggcca
     4621 gagtgctggc tgaggcaatg agccaggtca ccaatccagc cacaatcatg attcagaagg
     4681 gcaacttccg gaaccagcgc aaaaccgtga agtgctttaa ctgtggcaaa gaggggcata
     4741 tcgcaaagaa ttgcagggcc cctcggaaaa agggatgctg gaaatgtgga aaggaaggcc
     4801 accagatgaa ggactgtact gagagacagg ctaacttcct gggcaaaatt tggccctccc
     4861 ataaggggag acccgggaat tttctgcaga gccggcctga gccaaccgca ccacccgaag
     4921 agagtttcag gtttggcgaa gagactacca caccatcaca gaaacaggaa cccatcgaca
     4981 aggagctgta tcctctggca agtctgcgaa gcctgtttgg aagcgacccc tcatctcagg
     5041 gcggaagcgg cggatccatg gtctcaaagg gggaagagct gtttactggg gtcgtgccta
     5101 ttctggtcga actggacggg gatgtcaacg gccacaagtt tagtgtgagc ggagagggag
     5161 aaggcgacgc cacttacgga aagctgaccc tgaaattcat ttgcaccaca ggcaagctgc
     5221 cagtgccctg gcctaccctg gtcactaccc tgacatacgg cgtccagtgt tttagtaggt
     5281 atcctgacca catgaagcag catgatttct ttaaatcagc tatgccagag ggatacgtgc
     5341 aggaacgcac cattttcttt aaggacgatg gcaactacaa gactcgagca gaggtgaagt
     5401 tcgaagggga caccctggtc aaccggatcg agctgaaggg aatcgacttt aaagaagatg
     5461 gaaacatcct gggccacaag ctggagtaca actataattc tcataacgtg tacatcatgg
     5521 ccgataagca gaaaaacggg attaaggtca atttcaaaat cagacacaac atcgaagacg
     5581 gcagcgtgca gctggctgat cattaccagc agaacacacc aattggcgac gggccagtgc
     5641 tgctgccaga taatcactat ctgagcactc agtccgccct gtctaaggac cctaacgaga
     5701 aacgcgatca tatggtgctg ctggagttcg tcacagccgc tgggatcact ctgggaatgg
     5761 acgagctgta taaatgagaa ttctcgacct tgactggcgg cgcgaccttg aggcctgcgt
     5821 tcgcctcagt tgccccctct gtgcaatggg gagacgcgcc tcatcgcttg acaacggccg
     5881 aagagccgcc gcgcttccgt ctcccgcgtg cgcgcgccat gctgcccacc cccgttccgc
     5941 actgaccctc ccccgtgccc cgcgtcccgt actgccgccc cgccccgagt cccatgccgc
     6001 agccaccgcg acggagcccg caggcgggaa cctgcctccg cgcgttagcg cgcacgcgcg
     6061 cctcatgtgt cgtccccatc agcgccggct tccgtctata ggccagatgc actgtcactc
     6121 tggcgaagtc gcagacccga ttggccggga cggaggcgcg agaccgggtt gcgggcgggg
     6181 ccgaacgtgg tataaaaggg gcgggaggcc aggctcgtgc cgttttgcag acgccaccgc
     6241 cgaggaaaac cgtgtactat tagccacgcg tgccgccatg gctaaaccac tgtcccagga
     6301 agaatccaca ctgatcgaac gagcaaccgc tactatcaac tcaatcccaa tctccgaaga
     6361 ctactctgtg gcctccgccg ccctgagcag cgacggacga atcttcaccg gcgtgaacgt
     6421 ctaccacttt accggcggcc cctgcgctga gctggtggtc ctgggaactg cagccgctgc
     6481 agccgctgga aatctgacct gtatcgtggc tatcgggaac gaaaataggg gaatcctgtc
     6541 tccatgcggc cggtgtagac aggtcctgct ggatctgcat cccgggatca aggcaattgt
     6601 gaaagactca gatggacagc ccacagccgt cggcattcgc gagctgctgc ctagcggcta
     6661 tgtgtgggaa gggtgagcga tcgctaatca acctctggat tacaaaattt gtgaaagatt
     6721 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6781 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6841 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6901 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6961 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7021 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7081 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7141 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7201 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7261 ggccgcctcc ccgcttaatc gcgtcgagac ctagaaaaac atggagcaat cacaagtagc
     7321 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     7381 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     7441 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7501 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac
     7561 tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta
     7621 ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac
     7681 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac
     7741 agccgcctag catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc
     7801 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7861 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7921 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt
     7981 ttcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     8041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     8101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     8161 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     8221 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     8281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     8341 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     8401 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8461 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     8521 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8581 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8641 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8701 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8761 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8821 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8881 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8941 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     9001 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     9061 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     9121 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     9181 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     9241 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     9301 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     9361 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9421 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9481 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9541 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9601 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9661 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9721 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9781 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     9841 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     9901 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     9961 attgcatgaa g
//
