LOCUS       sequence-23237-e         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: pCGLex
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      235..422
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(290..314)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(574..593)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(575..593)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(600..617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(600..616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(609..631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttcgcaacgg cgactggctg gaattcagat ctactagtgc ggccgctaag taagtaagac
       61 gtcgagctct aagtaagtaa cggccgccac cgcggtggag ctttggactt cttcgccaga
      121 ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa
      181 aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat
      241 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
      301 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
      361 taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc
      421 ggcatgccga gcaaatgcct gcaaatcgct ccccatttca cccaattgta gatatgctaa
      481 ctccagcaat gagttgatga atctcggtgt gtattttatg tcctcagagg acaacacctg
      541 ttgtaatcgt tcttccacac ggatcccaat tcgccctata gtgagtcgta ttacaattca
      601 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      661 cttgcagcac atcccctttt cgccagctgg ngtaatagcg aagaggcccg caccgatcgc
      721 ccttcccaac agttgcgca
//
