LOCUS       sequence-232376-       10711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockdown pqsC in P. aeruginosa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1104
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  152..317
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             complement(473..562)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             complement(join(572..2155,2156..2158,2159..4645,4646..4648,
                     4649..4675))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             4687..4709
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(4736..5020)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             5047..5925
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(6347..6352,6353..6369,6370..6376)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    complement(6772..6819)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(6910..6915,6916..6932,6933..6938)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             7264..7500
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     protein_bind    complement(7568..7615)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(7666..7752)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(7855..7949)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(7977..7993)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7978..7994)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8408..8427)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8527..8549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8587..8605)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8673..8777
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8778..8846,8847..9638)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8996..9015)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9939..10327
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            10596..10704
                     /note="incP origin of transfer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcgg gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagcg
      121 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
      181 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
      241 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
      301 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
      361 agtgccatga tgctcattct gtgggaccaa aacgaaaaaa caccctttcg ggtgtctttt
      421 ctggaatttg gtaccgaggc tgcaagtccc attaaggagg cgcgccttat tacagatctt
      481 cctcagagat gagcttctgt tccagatctt cttctgaaat caacttttgt tccagatctt
      541 cttcagagat gagtttctgc tccgcggccg cgtcacctcc tagctgactc aaatcaatgc
      601 gtgtttcata aagaccagtg atggattgat ggataagagt ggcatctaaa acttcttttg
      661 tagacgtata tcgtttacga tcaattgttg tatcaaaata tttaaaagca gcgggagctc
      721 caagattcgt caacgtaaat aaatgaataa tattttctgc ttgttcacgt attggtttgt
      781 ctctatgttt gttatatgca ctaagaactt tatctaaatt ggcatctgct aaaataacac
      841 gcttagaaaa ttcactgatt tgctcaataa tctcatctaa ataatgctta tgctgctcca
      901 caaacaattg tttttgttcg ttatcttctg gactaccctt caacttttca taatgactag
      961 ctaaatataa aaaattcaca tatttgcttg gcagagccag ctcatttcct ttttgtaatt
     1021 ctccggcact agccagcatc cgtttacgac cgttttctaa ctcaaaaaga ctatatttag
     1081 gtagtttaat gattaagtct tttttaactt ccttatatcc tttagcttct aaaaagtcaa
     1141 tcggattttt ttcaaaggaa cttctttcca taattgtgat ccctagtaac tctttaacgg
     1201 attttaactt cttcgatttc cctttttcca ccttagcaac cactaggact gaataagcta
     1261 ccgttggact atcaaaacca ccatattttt ttggatccca gtctttttta cgagcaataa
     1321 gcttgtccga atttcttttt ggtaaaattg actccttgga gaatccgcct gtctgtactt
     1381 ctgttttctt gacaatattg acttggggca tggacaatac tttgcgcact gtggcaaaat
     1441 ctcgcccttt atcccagaca atttctccag tttccccatt agtttcgatt agagggcgtt
     1501 tgcgaatctc tccatttgca agtgtaattt ctgttttgaa gaagttcatg atattagagt
     1561 aaaagaaata ttttgcggtt gctttgccta tttcttgctc agacttagca atcattttac
     1621 gaacatcata aactttataa tcaccataga caaactccga ttcaagtttt ggatatttct
     1681 taatcaaagc agttccaacg acggcattta gatacgcatc atgggcatga tggtaattgt
     1741 taatctcacg tactttatag aattggaaat cttttcggaa gtcagaaact aatttagatt
     1801 ttaaggtaat cactttaacc tctcgaataa gtttatcatt ttcatcgtat ttagtattca
     1861 tgcgactatc caaaatttgt gccacatgct tagtgatttg gcgagtttca accaattggc
     1921 gtttgataaa accagcttta tcaagttcac tcaaacctcc acgttcagct ttcgttaaat
     1981 tatcaaactt acgttgagtg attaacttgg cgtttagaag ttgtctccaa tagtttttca
     2041 tctttttgac tacttcttca cttggaacgt tatccgattt accacgattt ttatcagaac
     2101 gcgttaagac cttattgtct attgaatcgt ctttaaggaa actttgtgga acaatggcat
     2161 cgacatcata atcacttaaa cgattaatat ctaattcttg gtccacatac atgtctcttc
     2221 cattttggag ataatagaga tagagctttt cattttgcaa ttgagtattt tcaacaggat
     2281 gctctttaag aatctgactt cctaattctt tgataccttc ttcgattcgt ttcatacgct
     2341 ctcgcgaatt tttctggccc ttttgagttg tctgattttc acgtgccatt tcaataacga
     2401 tattttctgg cttatgccgc cccattactt tgaccaattc atcaacaact tttacagtct
     2461 gtaaaatacc ttttttaata gcagggctac cagctaaatt tgcaatatgt tcatgtaaac
     2521 tatcgccttg tccagacact tgtgcttttt gaatgtcttc tttaaatgtc aaactatcat
     2581 catggatcag ctgcataaaa ttgcgattgg caaaaccatc tgatttcaaa aaatctaata
     2641 ttgttttgcc agattgctta tccctaatac cattaatcaa ttttcgagac aaacgtcccc
     2701 aaccagtata acggcgacgt ttaagctgtt tcatcacctt atcatcaaag aggtgagcat
     2761 atgttttaag tctttcctca atcatctccc tatcttcaaa taaggtcaat gttaaaacaa
     2821 tatcctctaa gatatcttca ttttcttcat tatccaaaaa atctttatct ttaataattt
     2881 ttagcaaatc atggtaggta cctaatgaag cattaaatct atcttcaact cctgaaattt
     2941 caacactatc aaaacattct atttttttga aataatcttc ttttaattgc ttaacggtta
     3001 cttttcgatt tgttttgaag agtaaatcaa caatggcttt cttctgttca cctgaaagaa
     3061 atgctggttt tcgcattcct tcagtaacat atttgacctt tgtcaattcg ttataaaccg
     3121 taaaatactc ataaagcaaa ctatgttttg gtagtacttt ttcatttgga agatttttat
     3181 caaagtttgt catgcgttca ataaatgatt gagctgaagc acctttatcg acaacttctt
     3241 caaaattcca tggggtaatt gtttcttcag acttccgagt catccatgca aaacgactat
     3301 tgccacgcgc caatggacca acataataag ggattcgaaa agtcaagatt ttttcaatct
     3361 tctcacgatt gtcttttaaa aatggataaa agtcttcttg tcttctcaaa atagcatgca
     3421 gctcacccaa gtgaatttga tggggaatag agccgttgtc aaaggtccgt tgcttgcgca
     3481 gcaaatcttc acgatttagt ttcaccaata attcctcagt accatccatt ttttctaaaa
     3541 ttggtttgat aaatttataa aattcttctt ggctagctcc cccatcaata taacctgcat
     3601 atccgttttt tgattgatca aaaaagattt ctttatactt ttctggaagt tgttgtcgaa
     3661 ctaaagcttt taaaagagtc aagtcttgat gatgttcatc gtagcgttta atcattgaag
     3721 ctgatagggg agccttagtt atttcagtat ttactcttag gatatctgaa agtaaaatag
     3781 catctgataa attcttagct gccaaaaaca aatcagcata ttgatctcca atttgcgcca
     3841 ataaattatc taaatcatca tcgtaagtat cttttgaaag ctgtaattta gcatcttctg
     3901 ccaaatcaaa atttgattta aaattagggg tcaaacccaa tgacaaagca atgagattcc
     3961 caaataagcc atttttcttc tcaccgggga gctgagcaat gagattttct aatcgtcttg
     4021 atttactcaa tcgtgcagaa agaatcgctt tagcatctac tccacttgcg ttaatagggt
     4081 tttcttcaaa taattgattg taggtttgta ccaactggat aaatagtttg tccacatcac
     4141 tattatcagg atttaaatct ccctcaatca aaaaatgacc acgaaactta atcatatgcg
     4201 ctaaggccaa atagattaag cgcaaatccg ctttatcagt agaatctacc aatttttttc
     4261 gcagatgata gatagttgga tatttctcat gataagcaac ttcatctact atatttccaa
     4321 aaataggatg acgttcatgc ttcttgtctt cttccaccaa aaaagactct tcaagtcgat
     4381 gaaagaaact atcatctact ttcgccatct catttgaaaa aatctcctgt agataacaaa
     4441 tacgattctt ccgacgtgta taccttctac gagctgtccg tttgagacga gtcgcttccg
     4501 ctgtctctcc actgtcaaat aaaagagccc ctataagatt ttttttgata ctgtggcggt
     4561 ctgtatttcc cagaaccttg aactttttag acggaacctt atattcatca gtgatcaccg
     4621 cccatccgac gctatttgtg ccgatagcta agcctattga gtatttctta tccatgggta
     4681 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag cccaaaaaaa cgggtatgga
     4741 gaaacagtag agagttgcga taaaaagcgt caggtaggat ccgctaatct tatggataaa
     4801 aatgctattg catagcaaag tgtgacgccg tgcaaataat caatgtggac ttttctgccg
     4861 tgattataga cacttttgtt acgcgttttt gtcatggctt tggtcccgct ttgttacaga
     4921 atgcttttaa taagcggggt taccggtttg gttagcgaga agagccagta aaagacgcag
     4981 tgacggcaat gtctgatgca atatggacaa ttggtttctt ctctgaatgg cgggagtatg
     5041 aaaagtatgg ctgaagcgca aaatgatccc ctgctgccgg gatactcgtt taatgcccat
     5101 ctggtggcgg gtttaacgcc gattgaggcc aacggttatc tcgatttttt tatcgaccga
     5161 ccgctgggaa tgaaaggtta tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa
     5221 aatcagggac gagaatttgt ttgccgaccg ggtgatattt tgctgttccc gccaggagag
     5281 attcatcact acggtcgtca tccggaggct cgcgaatggt atcaccagtg ggtttacttt
     5341 cgtccgcgcg cctactggca tgaatggctt aactggccgt caatatttgc caatacgggg
     5401 ttctttcgcc cggatgaagc gcaccagccg catttcagcg acctgtttgg gcaaatcatt
     5461 aacgccgggc aaggggaagg gcgctattcg gagctgctgg cgataaatct gcttgagcaa
     5521 ttgttactgc ggcgcatgga agcgattaac gagtcgctcc atccaccgat ggataatcgg
     5581 gtacgcgagg cttgtcagta catcagcgat cacctggcag acagcaattt tgatatcgcc
     5641 agcgtcgcac agcatgtttg cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag
     5701 ttagggatta gcgtcttaag ctggcgcgag gaccaacgta tcagccaggc gaagctgctt
     5761 ttgagcacca cccggatgcc tatcgccacc gtcggtcgca atgttggttt tgacgatcaa
     5821 ctctatttct cgcgggtatt taaaaaatgc accggggcca gcccgagcga gttccgtgcc
     5881 ggttgtgaag aaaaagtgaa tgatgtagcc gtcaagttgt cataaactag tactggctaa
     5941 gaggcatcac gattcgcttg cctatgagct ctgcagacgt aaaaaaagcg gcgtggttag
     6001 ccgctttttt aattgccgga gcgcaataaa aaagcccccg gaaggtgatc ttccgggggc
     6061 tttctcatgc gttgcttgta ttgaaaatgg gggccatgcc tacctatctg cctcccgggt
     6121 ccttgagccc cattatcacc tccaaagcct tctcgtctgg tcagtttcac ctgttttacg
     6181 taaaaacccg cttcggcggg tttttacttt tggggaaaca cagaaaaaag cccgcacctg
     6241 acagtgcggg cttttttttt cgaccaaagg tgcgactact cttgcctact acctatcgac
     6301 tgagctgaaa gaattccggt tctggcaaat attctgaaat gagctgttga caattaatca
     6361 tccggctcgt ataatgtgtg ggctgcgagc cggaagttca cgtttaagag ctatgctgga
     6421 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     6481 tcggtgcttt ttttttgaat tcatgtggct gaccgttctg ttgtctctcg ctcttccgag
     6541 tagacgaaca ataaggcctc cctaacgggg ggcctttttt attgataaca aaagtcagtg
     6601 cttccgctat ttccaaaata ccgggctaat acggtttaaa cgacctcctg gatttgctca
     6661 gacagccttt tcgtcattcg tttcagccaa aaaacttaag accgccggtc ttgtccacta
     6721 ccttgcagta atgcggtgga caggatcggc ggttttcttt tctcttctca agaagttcct
     6781 atactttcta gagaatagga acttcggaat aggaacttcc tcctgaacgg ccataagaac
     6841 gaaggctgtc tgttgaactc tcgagcctga ttccctttgt caacagcaat ggataattca
     6901 cgaacccagt tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc
     6961 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     7021 ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa
     7081 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     7141 ggcagtcgcc ctaaaacaaa gttaggcagc cgttgtgctg gtgctttctg atagttgttg
     7201 tggggtaggc agtcagagct cgatttgctt gtcgccataa tagattcaca agaaggattc
     7261 gacatgggtc aaagtagcga tgaagccaac gctcccgttg cagggcagtt tgcgcttccc
     7321 ctgagtgcca cctttggctt aggggatcgc gtacgcaaga aatctggtgc cgcttggcag
     7381 ggtcaagtcg tcggttggta ttgcacaaaa ctcactcctg aaggctatgc ggtcgagtcc
     7441 gaatcccacc caggctcagt gcaaatttat cctgtggctg cacttgaacg tgtggcctaa
     7501 gaattatcta gaattattcc attgagtaag tttttaagca ctcgagcatc agcttcaaaa
     7561 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaataggt acttcaagat
     7621 ccccaattcg agctcatcgt ccgggccgca agctcctagc ggcggatttg tcctactcag
     7681 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     7741 tcgttttatt tgatgcctca agctagagag tcattacccc aggcgtttaa gggcaccaat
     7801 aactgcctta aaaaaattac gccccgccct gccactcatc gcagtctagc ttggattctc
     7861 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     7921 ggtcattact ggatctatca acaggagtcc aagctcagct aattaagcta gcttatcgat
     7981 accgtcgacc tcgaacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa
     8041 atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga
     8101 ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct
     8161 attttgttca gtttaagact ttattgtccg cccacacctg cttacgcagg gcatccattt
     8221 attactcaac cgtaaccgat tttgccaggt tacgcggctg gtcctctagc tggcgtaata
     8281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     8341 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     8401 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     8461 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8521 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc gtcaccgaaa cgcgcgagac
     8581 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8641 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8701 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8761 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8821 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8881 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8941 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9001 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9061 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9121 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9181 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9241 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9301 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9361 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9421 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9481 ccggtgagcg tgggtcccgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9541 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9601 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9661 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9721 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9781 accccgtaga aaagatcaaa ggcagttatt ggtgcctcac tgattaagca ttggtaactg
     9841 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttttg
     9901 cggccgcaag atccggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     9961 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
    10021 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
    10081 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
    10141 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
    10201 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
    10261 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
    10321 gctgacatgg gaattagcca tggcatcaca gtatcgtgat gacagaggca gggagtgcgg
    10381 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10441 caggggattc cttaaggtat actttccgct gcataaccct gcttcggggt cattatagcg
    10501 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
    10561 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
    10621 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
    10681 aatcctgctc tgcgaggctg gccgataagc t
//
