LOCUS       sequence-232377-        8924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV-1 LTR driven reporter vector that retains complete LTRs, tat, 
            and rev, but has a frameshift mutation in env, an ngfr reporter gene
            in place of nef, and gfp in place of gag, pol, vif, and vpr .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, 
            Diehl WE, Luban J
  TITLE     Primate immunodeficiency virus proteins Vpx and Vpr counteract 
            transcriptional repression of proviruses by the HUSH complex.
  JOURNAL   Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 
            10.1038/s41564-018-0256-x. Epub 2018 Oct 8.
  PUBMED    30297740
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-018-0256-x"; journalName: "Nat Microbiol"; date: 
            "2018-12"; volume: "3"; issue: "12"; pages: "1354-1361"
COMMENT     Sequence Label: HIV-1  LTR-gfp
FEATURES             Location/Qualifiers
     source          1..8924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(240..260)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        245..574
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      425..560
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     487..506
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        586..965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        967..1165
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1120..1140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1183..1363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1410..1535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     CDS             join(1552..1554,1555..1557,1558..2268)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2311..2428
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4411..4644
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4829..4873
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             5167..5193
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     regulatory      5451..5460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     LTR             6471..7104
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7172..7760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7252..7271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7931..8722,8723..8791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8554..8573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8792..8896)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
      241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      541 aggaggcttt tttggaggcc taggcttttg caaaaagctc gcgttgacat tgattattga
      601 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      661 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      721 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      781 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      841 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      901 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      961 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     1021 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     1081 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     1141 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
     1201 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     1261 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     1321 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     1381 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     1441 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     1501 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcgggga gatggtgtca
     1561 aaaggagaag aactgttcac cggagtggtc ccaatcctgg tggagctgga cggagatgtc
     1621 aatggccaca agttcagcgt gagcggagag ggagaaggag acgctacata cggcaagctg
     1681 actctgaaat tcatttgcac cacagggaag ctgcccgtgc cttggccaac cctggtcact
     1741 accctgacat acggcgtcca gtgttttagc agatatccag accacatgaa gcagcatgat
     1801 ttctttaaat ccgccatgcc cgagggctac gtgcaggaac ggacaatctt ctttaaggac
     1861 gatggaaact ataaaactag agctgaggtg aagttcgaag gagacaccct ggtcaacagg
     1921 atcgagctga agggcattga ctttaaagaa gatgggaata ttctgggaca caagctggag
     1981 tacaactaca actctcataa cgtgtatatc atggcagata agcagaaaaa cggcattaag
     2041 gtcaatttca aaatccgcca caatattgaa gacgggagcg tgcagctggc cgatcattac
     2101 cagcagaaca cacctatcgg cgacggaccc gtgctgctgc ctgataatca ctatctgtct
     2161 actcagagtg ccctgtcaaa ggacccaaac gagaaacgag atcatatggt gctgctggag
     2221 tttgtcaccg ctgctggaat cacactgggc atggatgagc tgtacaagta acggccggcc
     2281 gtatacacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2341 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2401 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     2461 ttggttaatt aagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc
     2521 aggaagtcag cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg
     2581 ccaagtttgt ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca
     2641 gcgacgaaga gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt
     2701 agtacatgta atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat
     2761 agcaatagtt gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa
     2821 aatagacagg ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga
     2881 aggagaagta tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga
     2941 tattgatgat ctgtagtgct acagaaaaat tgtgggtcac cgtctattat ggggtacctg
     3001 tgtggaagga agcaaccacc actctatttt gtgcatcaga tgctaaagca tatatgatac
     3061 agaggtacat aatgtttggg ccacacatgc ctgtgtaccc acagacccca acccacaaga
     3121 agtagtattg gtaaatgtga cagaaaattt taacatgtgg aaaaatgaca tggtagaaca
     3181 gatgcatgag gatataatca gtttatggga tcaaagccta aagccatgtg taaaattaac
     3241 cccactctgt gttagtttaa agtgcactga tttgaagaat gatactaata ccaatagtag
     3301 tagcgggaga atgataatgg agaaaggaga gataaaaaac tgctctttca atatcagcac
     3361 aagcataaga gataaggtgc agaaagaata tgcattcttt tataaacttg atatagtacc
     3421 aatagataat accagctata ggttgataag ttgtaacacc tcagtcatta cacaggcctg
     3481 tccaaaggta tcctttgagc caattcccat acattattgt gccccggctg gttttgcgat
     3541 tctaaaatgt aataataaga cgttcaatgg aacaggacca tgtacaaatg tcagcacagt
     3601 acaatgtaca catggaatca ggccagtagt atcaactcaa ctgctgttaa atggcagtct
     3661 agcagaagaa gatgtagtaa ttagatctgc caatttcaca gacaatgcta aaaccataat
     3721 agtacagctg aacacatctg tagaaattaa ttgtacaaga cccaacaaca atacaagaaa
     3781 aagtatccgt atccagaggg gaccagggag agcatttgtt acaataggaa aaataggaaa
     3841 tatgagacaa gcacattgta acattagtag agcaaaatgg aatgccactt taaaacagat
     3901 agctagcaaa ttaagagaac aatttggaaa taataaaaca ataatcttta agcaatcctc
     3961 aggaggggac ccagaaattg taacgcacag ttttaattgt ggaggggaat ttttctactg
     4021 taattcaaca caactgttta atagtacttg gtttaatagt acttggagta ctgaagggtc
     4081 aaataacact gaaggaagtg acacaatcac actcccatgc agaataaaac aatttataaa
     4141 catgtggcag gaagtaggaa aagcaatgta tgcccctccc atcagtggac aaattagatg
     4201 ttcatcaaat attactgggc tgctattaac aagagatggt ggtaataaca acaatgggtc
     4261 cgagatcttc agacctggag gaggcgatat gagggacaat tggagaagtg aattatataa
     4321 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     4381 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     4441 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     4501 gtctgatata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     4561 gttgcaactc acagtctggg gcatcaaaca gctccaggca agaatcctgg ctgtggaaag
     4621 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     4681 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaataa
     4741 catgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     4801 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     4861 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     4921 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     4981 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caatcccgag
     5041 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     5101 cattcgatta gtgaacggat ccttagcact tatctgggac gatctgcgga gcctgtgcct
     5161 cttcagctac caccgcttga gagacttact cttgattgta acgaggattg tggaacttct
     5221 gggacgcagg gggtgggaag ccctcaaata ttggtggaat ctcctacaat attggagtca
     5281 ggagctaaag aatagtgctg ttagcttgct caatgccaca gccatagcag tagctgaggg
     5341 gacagatagg gttatagaag tagtacaagg agcttgtaga gctattcgcc acatacctag
     5401 aagaataaga cagggcttgg aaaggatttt gctataagat gggtggcgcg gccgccatgg
     5461 gggctggggc tactgggagg gctatggacg gaccaaggct gctgctgctg ctgctgctgg
     5521 gagtctcact ggggggggcc aaggaagcct gtcctactgg gctgtacacc cacagcggag
     5581 aatgctgtaa ggcttgtaac ctgggagagg gagtggctca gccttgcgga gcaaatcaga
     5641 ccgtctgcga gccatgtctg gactccgtga cattctctga tgtggtcagt gccactgaac
     5701 cttgcaaacc atgtaccgag tgcgtggggc tgcagtctat gagtgctccc tgtgtcgagg
     5761 ctgacgatgc agtgtgccgc tgtgcatacg gatactatca ggatgagacc acaggccgat
     5821 gtgaagcctg ccgggtctgt gaggctggat caggcctggt gtttagctgc caggataagc
     5881 agaacacagt gtgcgaggaa tgtccagacg gcacttattc cgatgaggcc aatcatgtcg
     5941 acccctgcct gccttgtacc gtgtgcgagg atacagaacg gcagctgaga gaatgtaccc
     6001 gatgggcaga cgccgagtgc gaggaaatcc ccgggaggtg gattacccgc tccacacccc
     6061 ctgaaggatc agacagcaca gcaccatcta ctcaggagcc agaagcacca ccagagcagg
     6121 atctgatcgc aagtactgtc gccggcgtgg tcactaccgt gatggggagc tcccagcccg
     6181 tggtcacaag aggcacaact gataacctga tccctgtgta ctgctctatt ctggcagctg
     6241 tggtcgtggg actggtggca tatattgctt tcaaaaggtg actcgagacc tagaaaaaca
     6301 tggagcaatc acaagtagca atacagcagc taacaatgct gcttgtgcct ggctagaagc
     6361 acaagaggag gaagaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     6421 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6481 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     6541 cttccctgat tggcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     6601 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     6661 gaacaccagc ttgttacacc ctgtgagcct gcatggaatg gatgaccctg agagagaagt
     6721 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     6781 ggagtacttc aagaactgct gacatcgagc ttgctacaag ggactttccg ctggggactt
     6841 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat gctgcatata
     6901 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7081 agtcagtgtg gaaaatctct agcaggcgtt tcatgtgagc aaaaggccag caaaaggcca
     7141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7861 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7921 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7981 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8041 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8101 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8161 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8221 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8281 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8341 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8401 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8461 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8521 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8581 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8641 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8701 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8761 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8821 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8881 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
