LOCUS       sequence-23238-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: pCALex
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      229..416
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(284..308)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(568..587)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(569..587)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(594..611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(594..610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(603..625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 acggcgactg gctggaattc agatctacta gtgcggccgc taagtaagta agacgtcgag
       61 ctctaagtaa gtaacggccg ccaccgcggt ggagctttgg acttcttcgc cagaggtttg
      121 gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg
      181 gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta
      241 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
      301 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
      361 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
      421 ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg ctaactccag
      481 caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaaca cctgttgtaa
      541 tcgttcttcc acacggatcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
      601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacntaa ancgccntgc
      661 agcacatccc cctttcgcca gct
//
