LOCUS       sequence-232381-        6956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full length of Catulin-GFP (Mus musculus). Contains CMV 
            and T3 promoter for mammalian expression and mRNA production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Gingras J, Gawor M, Bernadzki KM, Grady RM, Hallock P, Glass DJ, 
            Sanes JR, Proszynski TJ
  TITLE     Alpha-Dystrobrevin-1 recruits Grb2 and alpha-catulin to organize 
            neurotransmitter receptors at the neuromuscular junction.
  JOURNAL   J Cell Sci. 2016 Mar 1;129(5):898-911. doi: 10.1242/jcs.181180. Epub
            2016 Jan 14.
  PUBMED    26769899
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.181180"; journalName: "J Cell Sci"; date: "2016-03-1- 
            1"; volume: "129"; issue: "5"; pages: "898-911"
COMMENT     Sequence Label: pEGFP-N1-CATULIN
FEATURES             Location/Qualifiers
     source          1..6956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        634..652
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2902..2904,2905..2907,2908..3621)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2902..2904,2905..2907,2908..3618)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2947..2968)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3208..3227)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3555..3576
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3744..3865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3781..3800)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3835..3854
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3872..4327)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4009..4030)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4221..4240
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4354..4458
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4460..4817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4460..4651
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4483..4503)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4668..4803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4852..5646
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4852..5646
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4906..4925)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5516..5535
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5834..5853)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5878..5925
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6254..6842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6743..6762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attaattaac cctcactaaa ggacaccatg
      661 gccgcttctc cagtcccggg cggtggcggt gcgggagctg tccactcctc caacgcggct
      721 ggcttcacct tcgactcggg actggagatc aggacgcgct cggtggaaca gactcttctg
      781 cccctggttt ctcagatcac cacacttata aaccataaag ataataccaa aaagtctgat
      841 aaaaccctgc aagcgattca gcgtgtggga caagcagtca acttggcagt tggaagattt
      901 gttaaagtag gggaagctat agccaacgaa aactgggatt tgaaagaaga aattaacatc
      961 gcctgcatcg aagccaagca agcaggtgaa acaatcgcct cactcacaga tgtgactaaa
     1021 cggagccatt tggaatcaga tggtcaggtc acgattctga cagacaaaac aggcgtcgtg
     1081 caggccgccc ggctgctgct ctcctcagtg acaaaagtgc tgctgctggc agatcgtgta
     1141 gtcatcaagc agatagtgac atcgagaaac aagattctcg caaccatgga aaggctagag
     1201 aaagtaaaca gctttcagga atttgttcag atattcagcc agtttggaaa cgaaatggtg
     1261 gagtttgcac atctaacggg agaccgacaa aatgatttga aagatgagaa gaaaaaggcc
     1321 agaatggccg tggcgagggc cgtgcttgaa aagggtacga tgatgctcct cacagcctca
     1381 aagacatgtc tcaggcatcc cagctgtgag tcagcccaca caaacaaaga aggagtgttt
     1441 gaccggatga gagtggccct ggagaaggtc acggagatcg tgactgactg cagactcagc
     1501 ggagagaccg acagctcatc tgtcagcatt tttactggga ttaaagagtt gaaggtgaat
     1561 attgaagctc ttcgggagaa tgtttgcttt gagtccaaag agaacctttc tgccgcattg
     1621 gaggccgtct tggagcatgt ggaggacttc actgattctg cctacaccag ccacgagcac
     1681 agggaacgga tcttggagct gtcttctcag gcgaggacag agctgcagca gctcctttcc
     1741 gtgtggatgc aaacccaaag caggaagaca aagagcgccg ccgaggagct ggaactcaca
     1801 gttctgaaga tcagccacag cctggacgag ctgaggagag aactccattg tacagccatg
     1861 cagctggcag ccgacctgtt aaaattccat gctgatcatg tggttttaaa agcattaaaa
     1921 gttactggag tagaaggaaa tttggaagct ttggctgagt atgcctgtaa actctctgag
     1981 cagaaagaac agcttgtgga gacatgtcga ttgttgcggc atatatccgg cacagaaccc
     2041 cttgagataa cgtgcataca tgcagaggag acatttcagg tgactggcca acagatcatt
     2101 tctgctgctg aaacgttgac gttgcatccg tctagcaaaa tcgctaagga aaacctggat
     2161 gtgttttgtg aagcttggga gtctcagatg agtgacatgg cgacgctgct aagggaaatc
     2221 agtgatgtgt ttgaaggaag acgaggagag aggtgcgacc atctttcact tccaaagccc
     2281 acgaagaata gtgcaaacct gaagtcacta aagcccgaca aacctgactc ggaggagcaa
     2341 gccaagatag caaagctcgg gctgaagctg ggattgctct cctctgatgc tgactgtgaa
     2401 atcgagaaat gggaagatga agagaacgag atcgttcggc atggacgcaa tatgtccagg
     2461 atggcctatt ctctgtattt atttactaga ggagaggggc cactgaaaac ttcccaggac
     2521 ttaattcact tcctagaggt ttttgctgca gagggattaa agcttacttc aagtgtacag
     2581 tcattttcaa aacagctaaa agatgatgac aaacttatgc ttctcctgga aataaacaaa
     2641 cttattcctc tatgccacca gcttcagaca ataactaaga catctttgca gagtaaagtc
     2701 tttctaaagg ttgacaagtg tattacaaag ataagatcca tgatgactct tgtggtccag
     2761 ctcctctcac tttgctataa actactgaag aagatggaaa ataacagatg gggctctgcg
     2821 acgaacaaag ataccatgga tggtcaaaac cgaattctgc agtcgacggt accgcgggcc
     2881 cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     2941 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3001 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3061 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3121 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3181 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3241 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3301 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3361 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3421 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3481 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3541 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3601 atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
     3661 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     3721 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     3781 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3841 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     3901 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     3961 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4021 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4081 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4141 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4201 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4261 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4321 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4381 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4441 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     4501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4561 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4621 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4681 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4741 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4801 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     4861 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4921 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4981 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     5041 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5101 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5161 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5221 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5281 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5341 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     5401 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5461 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5521 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5581 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5641 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5701 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5761 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     5821 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     5881 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     5941 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     6001 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     6061 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     6121 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6181 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6421 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
