LOCUS       sequence-232382-        9461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction intermediate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1106
FEATURES             Location/Qualifiers
     source          1..9461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            8..116
                     /note="incP origin of transfer"
     rep_origin      385..773
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(join(1074..1865,1866..1934))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1697..1716
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1935..2039)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2107..2125
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2163..2185)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2285..2304
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2718..2734
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2719..2735
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2763..2857
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      2960..3046
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    3097..3144
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3212..3448)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(3774..3779,3780..3796,3797..3802))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    3893..3940
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        complement(join(4336..4342,4343..4359,4360..4365))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             join(4787..4813,4814..4816,4817..7303,7304..7306,
                     7307..8890)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8900..8989
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     mobile_element  9145..9310
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 agcttatcgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
       61 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
      121 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
      181 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
      241 cagcggaaag tataccttaa ggaatcccct gataacgcag gaaagaacat gtgagcaaaa
      301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcactcc ctgcctctgt catcacgata
      361 ctgtgatgcc atggctaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      421 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      481 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      541 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      601 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
      661 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
      721 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
      781 ccggacgcat cgtggccgga tcttgcggcc gcaaaaatta aaaatgaagt tttaaatcaa
      841 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      901 caataactgc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
      961 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1021 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1081 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1141 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1201 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1261 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1321 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1381 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1441 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1501 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1561 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1621 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1681 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1741 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1801 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1861 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1921 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2041 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2101 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga cgacggtgaa
     2161 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     2221 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     2281 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     2341 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     2401 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagga ccagccgcgt
     2461 aacctggcaa aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcaggtgtgg
     2521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     2581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     2641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     2701 aaagaggggc gtggggttcg aggtcgacgg tatcgataag ctagcttaat tagctgagct
     2761 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt tgttcagaac
     2821 gctcggttgc cgccgggcgt tttttattgg tgagaatcca agctagactg cgatgagtgg
     2881 cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg
     2941 actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     3001 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgctaggagc
     3061 ttgcggcccg gacgatgagc tcgaattggg gatcttgaag tacctattcc gaagttccta
     3121 ttctctagaa agtataggaa cttcagagcg cttttgaagc tgatgctcga gtgcttaaaa
     3181 acttactcaa tggaataatt ctagataatt cttaggccac acgttcaagt gcagccacag
     3241 gataaatttg cactgagcct gggtgggatt cggactcgac cgcatagcct tcaggagtga
     3301 gttttgtgca ataccaaccg acgacttgac cctgccaagc ggcaccagat ttcttgcgta
     3361 cgcgatcccc taagccaaag gtggcactca ggggaagcgc aaactgccct gcaacgggag
     3421 cgttggcttc atcgctactt tgacccatgt cgaatccttc ttgtgaatct attatggcga
     3481 caagcaaatc gagctctgac tgcctacccc acaacaacta tcagaaagca ccagcacaac
     3541 ggctgcctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     3601 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     3661 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     3721 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     3781 gaaccgaaca ggcttatgtc aactgggttc gtgaattatc cattgctgtt gacaaaggga
     3841 atcaggctcg agagttcaac agacagcctt cgttcttatg gccgttcagg aggaagttcc
     3901 tattccgaag ttcctattct ctagaaagta taggaacttc ttgagaagag aaaagaaaac
     3961 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt
     4021 tttggctgaa acgaatgacg aaaaggctgt ctgagcaaat ccaggaggtc gtttaaaccg
     4081 tattagcccg gtattttgga aatagcggaa gcactgactt ttgttatcaa taaaaaaggc
     4141 cccccgttag ggaggcctta ttgttcgtct actcggaaga gcgagagaca acagaacggt
     4201 cagccacatg aattcaaaaa aaaagcaccg actcggtgcc actttttcaa gttgataacg
     4261 gactagcctt atttaaactt gctatgctgt ttccagcata gctcttaaac agaccgctaa
     4321 actgaaagtt ccacacatta tacgagccgg atgattaatt gtcaacagct catttcagaa
     4381 tatttgccag aaccggaatt ctttcagctc agtcgatagg tagtaggcaa gagtagtcgc
     4441 acctttggtc gaaaaaaaaa gcccgcactg tcaggtgcgg gcttttttct gtgtttcccc
     4501 aaaagtaaaa acccgccgaa gcgggttttt acgtaaaaca ggtgaaactg accagacgag
     4561 aaggctttgg aggtgataat ggggctcaag gacccgggag gcagataggt aggcatggcc
     4621 cccattttca atacaagcaa cgcatgagaa agcccccgga agatcacctt ccgggggctt
     4681 ttttattgcg ctccggcaat taaaaaagcg gctaaccacg ccgctttttt tacgtctgca
     4741 gagctcatag gcaagcgaat cgtgatgcct cttagccagt actagtatgg ataagaaata
     4801 ctcaataggc ttagctatcg gcacaaatag cgtcggatgg gcggtgatca ctgatgaata
     4861 taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca gtatcaaaaa
     4921 aaatcttata ggggctcttt tatttgacag tggagagaca gcggaagcga ctcgtctcaa
     4981 acggacagct cgtagaaggt atacacgtcg gaagaatcgt atttgttatc tacaggagat
     5041 tttttcaaat gagatggcga aagtagatga tagtttcttt catcgacttg aagagtcttt
     5101 tttggtggaa gaagacaaga agcatgaacg tcatcctatt tttggaaata tagtagatga
     5161 agttgcttat catgagaaat atccaactat ctatcatctg cgaaaaaaat tggtagattc
     5221 tactgataaa gcggatttgc gcttaatcta tttggcctta gcgcatatga ttaagtttcg
     5281 tggtcatttt ttgattgagg gagatttaaa tcctgataat agtgatgtgg acaaactatt
     5341 tatccagttg gtacaaacct acaatcaatt atttgaagaa aaccctatta acgcaagtgg
     5401 agtagatgct aaagcgattc tttctgcacg attgagtaaa tcaagacgat tagaaaatct
     5461 cattgctcag ctccccggtg agaagaaaaa tggcttattt gggaatctca ttgctttgtc
     5521 attgggtttg acccctaatt ttaaatcaaa ttttgatttg gcagaagatg ctaaattaca
     5581 gctttcaaaa gatacttacg atgatgattt agataattta ttggcgcaaa ttggagatca
     5641 atatgctgat ttgtttttgg cagctaagaa tttatcagat gctattttac tttcagatat
     5701 cctaagagta aatactgaaa taactaaggc tcccctatca gcttcaatga ttaaacgcta
     5761 cgatgaacat catcaagact tgactctttt aaaagcttta gttcgacaac aacttccaga
     5821 aaagtataaa gaaatctttt ttgatcaatc aaaaaacgga tatgcaggtt atattgatgg
     5881 gggagctagc caagaagaat tttataaatt tatcaaacca attttagaaa aaatggatgg
     5941 tactgaggaa ttattggtga aactaaatcg tgaagatttg ctgcgcaagc aacggacctt
     6001 tgacaacggc tctattcccc atcaaattca cttgggtgag ctgcatgcta ttttgagaag
     6061 acaagaagac ttttatccat ttttaaaaga caatcgtgag aagattgaaa aaatcttgac
     6121 ttttcgaatc ccttattatg ttggtccatt ggcgcgtggc aatagtcgtt ttgcatggat
     6181 gactcggaag tctgaagaaa caattacccc atggaatttt gaagaagttg tcgataaagg
     6241 tgcttcagct caatcattta ttgaacgcat gacaaacttt gataaaaatc ttccaaatga
     6301 aaaagtacta ccaaaacata gtttgcttta tgagtatttt acggtttata acgaattgac
     6361 aaaggtcaaa tatgttactg aaggaatgcg aaaaccagca tttctttcag gtgaacagaa
     6421 gaaagccatt gttgatttac tcttcaaaac aaatcgaaaa gtaaccgtta agcaattaaa
     6481 agaagattat ttcaaaaaaa tagaatgttt tgatagtgtt gaaatttcag gagttgaaga
     6541 tagatttaat gcttcattag gtacctacca tgatttgcta aaaattatta aagataaaga
     6601 ttttttggat aatgaagaaa atgaagatat cttagaggat attgttttaa cattgacctt
     6661 atttgaagat agggagatga ttgaggaaag acttaaaaca tatgctcacc tctttgatga
     6721 taaggtgatg aaacagctta aacgtcgccg ttatactggt tggggacgtt tgtctcgaaa
     6781 attgattaat ggtattaggg ataagcaatc tggcaaaaca atattagatt ttttgaaatc
     6841 agatggtttt gccaatcgca attttatgca gctgatccat gatgatagtt tgacatttaa
     6901 agaagacatt caaaaagcac aagtgtctgg acaaggcgat agtttacatg aacatattgc
     6961 aaatttagct ggtagccctg ctattaaaaa aggtatttta cagactgtaa aagttgttga
     7021 tgaattggtc aaagtaatgg ggcggcataa gccagaaaat atcgttattg aaatggcacg
     7081 tgaaaatcag acaactcaaa agggccagaa aaattcgcga gagcgtatga aacgaatcga
     7141 agaaggtatc aaagaattag gaagtcagat tcttaaagag catcctgttg aaaatactca
     7201 attgcaaaat gaaaagctct atctctatta tctccaaaat ggaagagaca tgtatgtgga
     7261 ccaagaatta gatattaatc gtttaagtga ttatgatgtc gatgccattg ttccacaaag
     7321 tttccttaaa gacgattcaa tagacaataa ggtcttaacg cgttctgata aaaatcgtgg
     7381 taaatcggat aacgttccaa gtgaagaagt agtcaaaaag atgaaaaact attggagaca
     7441 acttctaaac gccaagttaa tcactcaacg taagtttgat aatttaacga aagctgaacg
     7501 tggaggtttg agtgaacttg ataaagctgg ttttatcaaa cgccaattgg ttgaaactcg
     7561 ccaaatcact aagcatgtgg cacaaatttt ggatagtcgc atgaatacta aatacgatga
     7621 aaatgataaa cttattcgag aggttaaagt gattacctta aaatctaaat tagtttctga
     7681 cttccgaaaa gatttccaat tctataaagt acgtgagatt aacaattacc atcatgccca
     7741 tgatgcgtat ctaaatgccg tcgttggaac tgctttgatt aagaaatatc caaaacttga
     7801 atcggagttt gtctatggtg attataaagt ttatgatgtt cgtaaaatga ttgctaagtc
     7861 tgagcaagaa ataggcaaag caaccgcaaa atatttcttt tactctaata tcatgaactt
     7921 cttcaaaaca gaaattacac ttgcaaatgg agagattcgc aaacgccctc taatcgaaac
     7981 taatggggaa actggagaaa ttgtctggga taaagggcga gattttgcca cagtgcgcaa
     8041 agtattgtcc atgccccaag tcaatattgt caagaaaaca gaagtacaga caggcggatt
     8101 ctccaaggag tcaattttac caaaaagaaa ttcggacaag cttattgctc gtaaaaaaga
     8161 ctgggatcca aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt
     8221 ggttgctaag gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg
     8281 gatcacaatt atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa
     8341 aggatataag gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga
     8401 gttagaaaac ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga
     8461 gctggctctg ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt
     8521 gaagggtagt ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta
     8581 tttagatgag attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc
     8641 caatttagat aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca
     8701 agcagaaaat attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa
     8761 atattttgat acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc
     8821 cactcttatc catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct
     8881 aggaggtgac gcggccgcgg agcagaaact catctctgaa gaagatctgg aacaaaagtt
     8941 gatttcagaa gaagatctgg aacagaagct catctctgag gaagatctgt aataaggcgc
     9001 gcctccttaa tgggacttgc agcctcggta ccaaattcca gaaaagacac ccgaaagggt
     9061 gttttttcgt tttggtccca cagaatgagc atcatggctc tagtcgacat cggagaagag
     9121 tacggctctt ttaaccgcct caagaaccag ataagtgaaa tctagttcca aactattttg
     9181 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     9241 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     9301 tccgcccaca gcggggcatt tttcttcctg ttatgtttgg gcgctgcatt aatgaatcgg
     9361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     9421 cccgctgcgc tcggtcgttc ggctgcggcg agcggtatca g
//
