LOCUS       sequence-23239-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: pCGB42
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..252
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /label=B42 transcriptional activator
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
     CDS             259..285
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(311..327)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(311..327)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(538..562)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 agaaaggtag ctggtatcaa taaagatatc gaggagtgca atgccatcat tgagcagttt
       61 atcgactacc tgcgcaccgg acaggagatg ccgatggaaa tggcggatca ggcgattaac
      121 gtggtgccgg gcatgacgcc gaaaaccatt cttcacgccg ggccgccgat ccagcctgac
      181 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
      241 ctgagtggag atgcctccta cccttatgat gtgccagatt atgcctctcc cgaattcctg
      301 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ttggacttct
      361 tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa
      421 tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat
      481 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
      541 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttaatt cttgagtnac
      601 tctttcctgt aggtcaggtt gct
//
