LOCUS       sequence-232405-        4102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli attTn7 site with B. subtilis flanking sequence cloned into 
            pACYC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1273
FEATURES             Location/Qualifiers
     source          1..4102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1072..1174
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1175..1834
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1241..1260)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
      901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
      961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1861 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     1921 gaaagcaaat tcgacccggt cgtcggttca gggcagggtc gttaaatgtt atgcgcaaaa
     1981 tcatccaaac gtatcaagat gaaaacggca agctgtctgt gcctggcgat atcgctgccg
     2041 ctgtagcgga agcggaccgc atctatatca ttggctgcgg aacaagctac catgcaggac
     2101 ttgtcggtaa acaatatatt gaaatgtggg caaacgtgcc ggttgaagtg catgtagcga
     2161 gtgaattctc ctacaacatg ccgcttctgt ctaagaaacc gctcttcatt ttcctttctc
     2221 aaagcggaga aacagcagac agccgcgcgg tactcgttca agtcaaagcg ctcggacaca
     2281 aagccctgac aatcacaaac gtacctggat caacgctttc tcgtgaagct gactatacat
     2341 tgctgcttca tgcaggccct gagatcgctg ttgcgtcaac gaaagcatac actgcacaaa
     2401 tcgcagttct ggcggttctt gcttctgtgg ctgctgacaa aaatggcatc aatatcggat
     2461 ttgacctcgt caaagaactc ggtatcgctg caaacgcaat ggaagctcta tgcgaccaga
     2521 aagacgaaat ggaaatgatc gctcgtgaat acctgactgt atccagaaat gctttcttca
     2581 tcggacgcgg ccttgactac ttcgtatgtg tcgaaggcgc actgaagctg aaagagattt
     2641 cttacatcca ggcagaaggt tttgccggcg gtgagctaaa gcacggaacg attgccttga
     2701 tcgaacaagg aacaccagta ttcgcactgg caactcaaga gcatgtaaac ctaagcatcc
     2761 gcggaaacgt caaagaagtt gctgctcgcg gagcaaacac atgcatcatc tcactgaaag
     2821 gcctagacga tgcggatgac agattcgtat tgccggaagt aaacccagcg cttgctccgt
     2881 tggtatctgt tgttccattg cagctgatcg cttactatgc tgcactgcat cgcggctgtg
     2941 atgtggataa acctcgtaac ctggcaaaat cggttacggt tgagtaataa atgtttaacc
     3001 cctttggata agattatcta aaggggtgtt tttatgtcca aaagcgaaag aacgtctaca
     3061 gtggaaaaat ggattaaaga agttgatggt acaggacccg actatcgatc gaccatggct
     3121 atagatttaa gatgtttcct cattagatcg gtcaatcagg ttaaaaggca ttaaaacttg
     3181 gagagaaact gcttttgcct aactgagttt tctaatgtta atacagatat aagagagcag
     3241 ttcctcatta agatacgctt ttgatagcag ttggaattta gcatgctaga aatccgaaaa
     3301 ccggagagcc tattggaatg ataacgtgtt tttttattga caattaataa agggcaaaga
     3361 attctagaag tcgcacttac aatgtacttg tgggattagg ttttaaagtt cattcttttt
     3421 ttattgagta cattccggtt aaagttgaaa tgattcagta aaggacacgc cataaataaa
     3481 aggtagcgta tgattgccat tctttccatg gaaccgatat ttctaagatt tcatctttcg
     3541 taggctttcg gttcatatta cgtaatattt ttatcgaatg aataagaccc acatcatcga
     3601 ttggaaaagc tgtcgggaac ctaaggcagc gcattagaac ataattggct gtccaaggac
     3661 cgattcctcg tattttaatt aagttttttt cagcatcttt aaagttcatt ttcattaatt
     3721 tttccctcga taattctcca cttgccatta atctggctat tccaatgata tattcacttt
     3781 ttttcaccgt cattttaata tctgccaggt cggtaggggt taaccgtgca attcgctcgt
     3841 acggtgggaa cacccaatac tttttaccat tccattcaat agaatcgcca aatgcttcta
     3901 caaattgctt ctttaaggag tacgcgaagg ctaagttaat ttgttgccct aaaactcccc
     3961 aacataaagc ttcaaataaa tcgggaatgc cgatcactcg caacccataa aattttcggg
     4021 ggctgacttc aggtgctaca tttgaagaga taaattgcac tgaaatctag aaatatttta
     4081 tctgattaat aagatgatct tc
//
