LOCUS       sequence-232409-        6749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Septin 11 isoform II mammalian expression (EGFP-tag at the 
            N-terminus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Li X, Serwanski DR, Miralles CP, Nagata K, De Blas AL
  TITLE     Septin 11 is present in GABAergic synapses and plays a functional 
            role in the cytoarchitecture of neurons and GABAergic synaptic 
            connectivity.
  JOURNAL   J Biol Chem. 2009 Jun 19;284(25):17253-65. doi: 
            10.1074/jbc.M109.008870. Epub 2009 Apr 20.
  PUBMED    19380581
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.008870"; journalName: "J Biol Chem"; date: 
            "2009-06-19- 19"; volume: "284"; issue: "25"; pages: "17253-65"
COMMENT     Sequence Label: EGFP-Septin 11  isoform II
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3537..3658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3574..3593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3628..3647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3665..4120)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3802..3823)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4014..4033
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4147..4251
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4253..4610
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4253..4444
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4276..4296)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4461..4596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4645..5439
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4645..5439
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4699..4718)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5309..5328
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5627..5646)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5671..5718
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6047..6635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6536..6555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctatggcc gtggcggtgg ggagaccgag taatgaagaa
     1381 ctgcgaaact tatcactttc tggccatgtt ggatttgaca gtctccctga ccagctggtc
     1441 aacaaatcca cttctcaagg attctgtttc aacatcctgt gtgtgggaga gacaggtatt
     1501 ggcaaatcca cattgatgga cactttattc aacaccaaat ttgaaagtga cccagctact
     1561 cacaacgaac caggtgtccg gttaaaagcc agaagttacg aactgcagga aagcaacgta
     1621 cggctgaagc taaccattgt tgacacagtg ggatttggag accagattaa taaagacgac
     1681 agctataagc ccatagtgga gtacattgac gcccagtttg aggcctactt gcaagaggaa
     1741 ttgaaaatta agcgttctct cttcaactac cacgacacga ggattcacgc ctgcctttac
     1801 tttatcgccc ccacgggaca ttcactgaag tccctggacc tggtcaccat gaagaagctg
     1861 gacagcaagg tgaacatcat cccaatcatt gcgaaagccg acaccatcgc caagaacgaa
     1921 ttgcacaagt tcaagagtaa gatcatgagt gaactcgtga gcaacggagt ccagatatat
     1981 cagttcccca ccgatgagga aacggtggcc gagattaatg caacgatgag tgtccatctc
     2041 ccatttgcag tggttggcag taccgaagag gtgaagattg gcaacaagat ggcgaaggcc
     2101 aggcagtacc cttggggtgt ggtgcaggtt gagaacgaaa accactgtga ttttgtgaag
     2161 ctgagggaga tgctgatccg agtgaacatg gaggacctgc gagagcagac gcacactcgc
     2221 cactacgagt tgtatcggcg ctgtaagctc gaggagatgg gcttcaagga cactgaccct
     2281 gacagcaagc ccttcagtct tcaggagaca tatgaagcaa agaggaatga gttcctggga
     2341 gagctgcaga agaaggagga ggagatgaga cagatgtttg tcatgagagt gaaggagaaa
     2401 gaggcggagc tcaaggaggc ggagaaagag cttcatgaga agtttgacct cctgaagcgg
     2461 actcaccaag aggagaagaa aaaggtggaa gacaagaaga aggagcttga ggaggaggtg
     2521 agcaacttcc agaagaagaa ggcggcggct cagctcttgc agtcgcaggc ccagcagtct
     2581 ggggcccagc agaccaagaa ggacaaggat aagaaaaata gtccctggct ctgcacggaa
     2641 taaacgatac tctcaaatct aactggacgt gctttcgcct tcgcatcttc ttctttatgt
     2701 agccatcacg ttgccaagcc ctactctctc ttaatggaca gcattagaag agcgggagct
     2761 gtctgttcac gagttgggga agagttacct catctccacc atcgcctccc attctttaaa
     2821 gtctgggtca aacattgcac tgctgtttgt aattgtcaat gtctgtttac aaaccaccac
     2881 cctcagtggc taccgaagtg acttcaacta ccaaacaaaa taacaataat attaagagtg
     2941 gaatagaaaa tgtaggccca aacctgggga cttgggctgg aatcctcttg cttattagct
     3001 gccatttctt gaacctcctg atctaagacc ttatggaccg aagagagcat gtacctcact
     3061 tgaggttgtc atcacctgca gggcaaacat agcacaaggt accccctggt ctctatccta
     3121 tgggcaaatt tcaccaaccc catcaggatc taaagctctt agcaggtcat cagagcgggg
     3181 caacccttgc tgcctcctat tggttttgtg ggttgttctg gtgtgggatt ttagaaaagg
     3241 aaaaaaaaaa aaagaaaggt attgcatgca cagaggtgcc tgcagttttc cctctggaac
     3301 gtcctgttga tacggtgttt ttatttttca gctgaccatc tgcctcttga gagaaagaag
     3361 tgggcatcca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggat
     3421 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3481 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3541 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3601 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac
     3661 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     3721 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3781 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3841 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3901 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3961 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4021 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4081 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     4141 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg
     4261 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4321 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4381 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4441 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4501 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4561 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa
     4621 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4681 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4741 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4801 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     4861 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4921 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     4981 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     5041 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     5101 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     5161 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     5221 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     5281 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     5341 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     5401 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     5461 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     5521 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5581 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg
     5641 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg
     5701 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     5761 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5821 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5881 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5941 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6001 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6061 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6121 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6181 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6241 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6301 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6361 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6421 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6481 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6541 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6601 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6661 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6721 ctgtggataa ccgtattacc gccatgcat
//
