LOCUS       sequence-23241-e         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Huang J, Schreiber SL
  TITLE     A yeast genetic system for selecting small molecule inhibitors of 
            protein-protein interactions in nanodroplets.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 9. 94(25):13396-401.
  PUBMED    9391035
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Dec 9. 94(25):13396-401."
COMMENT     Sequence Label: pCAB42
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             153..173
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             180..416
                     /codon_start=1
                     /product="yeast transcriptional activator cretaed from E. 
                     coli genomic DNA fragments (Ma and Ptashne, 1987)"
                     /label=B42 transcriptional activator
                     /translation="INKDIEECNAIIEQFIDYLRTGQEMPMEMADQAINVVPGMTPKTI
                     LHAGPPIQPDWLKSNGFHEIEADVNDTSLLLSGD"
     CDS             423..449
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(475..491)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(475..491)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ctgtcttgct atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt
       61 gttctcgttc cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc
      121 atacaatcaa ctccaagctt atgggtgctc ctccaaaaaa gaagagaaag gtagctggta
      181 tcaataaaga tatcgaggag tgcaatgcca tcattgagca gtttatcgac tacctgcgca
      241 ccggacagga gatgccgatg gaaatggcgg atcaggcgat taacgtggtg ccgggcatga
      301 cgccgaaaac cattcttcac gccgggccgc cgatccagcc tgactggctg aaatcgaatg
      361 gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatgcct
      421 cctaccctta tgatgtgcca gattatgcct ctcccgaatt cctgcagccc gggggatcca
      481 ctagttctag agcggccgcc accgcggtgg agctttggac ttcttcgcca gaggtttggt
      541 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
      601 aaagggtcaa atcgttggta gatacgttgt tgacacttct aannnagcga atttcttatg
      661 att
//
