LOCUS       sequence-232411-        9968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Tn7 transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1039
FEATURES             Location/Qualifiers
     source          1..9968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1470..1826
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            1877..1985
                     /note="incP origin of transfer"
     primer_bind     2226..2243
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2360..2381
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2396..2401,2402..2419,2420..2426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2434..2450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2439..2461
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2458..2474
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2458..2474
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        join(2499..2504,2505..2521,2522..2527)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     mobile_element  3038..3262
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     primer_bind     complement(9574..9591)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9574..9590)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9583..9605)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9799..9818)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9918..9940
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagccggtt gtcagccgtt aagtgttcct gtgtcactca
     1501 aaattgcttt gagaggctct aagggcttct cagtgcgtta catccctggc ttgttgtcca
     1561 caaccgttaa accttaaaag ctttaaaagc cttatatatt cttttttttc ttataaaact
     1621 taaaacctta gaggctattt aagttgctga tttatattaa ttttattgtt caaacatgag
     1681 agcttagtac gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc
     1741 catgagggtt tagttcgtta aacatgagag cttagtacgt taaacttgag agcttagtac
     1801 gtgaaacatg agagcttagt acgtactatc aacaggttga actgcccatg ttctttcctg
     1861 cgttatcaga gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
     1921 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
     1981 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
     2041 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
     2101 agggttatgc agcggaaagt ataccttaaa tgttctttcc tgcgttatcc cctgattctg
     2161 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2221 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2281 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2341 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2401 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2461 gaaacagcta tgacatgatt acgaattcac gaacccagtt gacataagcc tgttcggttc
     2521 gtaaactgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2581 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgtac
     2641 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     2701 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaggcagcc
     2761 gttgtgctgg tgctttctga tagttgttgt ggggtaggca gtcagagctc gatttgcttg
     2821 tcgccataat agattcacaa gaaggattcg acccggggat cctctagagt cgagatgccg
     2881 catgtggaag aggtgattgc accgatcttc tacaccgttc cgctgcagct gctggcttac
     2941 catgtcgcgc tgatcaaagg caccgacgtt gaccagccgc gtaacctggc aaaatcggtt
     3001 acggttgagt aataaatgga tgccctgcgt aagcgggtgt gggcggacaa taaagtctta
     3061 aactgaacaa aatagatcta aactatgaca ataaagtctt aaactagaca gaatagttgt
     3121 aaactgaaat cagtccagtt atgctgtgaa aaagcatact ggacttttgt tatggctaaa
     3181 gcaaactctt cattttctga agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa
     3241 gggcatggta aagactatat tccatggcta acagtacaag aagttccttc ttcaggtcgt
     3301 tcccaccgta tttattctca taagacggga cgagtccatc atttgctatc tgacttagag
     3361 cttgctgttt ttctcagtct tgagtgggag agcagcgtgc tagatatacg cgagcagttc
     3421 cccttattac ctagtgatac caggcagatt gcaatagata gtggtattaa gcatcctgtt
     3481 attcgtggtg tagatcaggt tatgtctact gattttttag tggactgcaa agatggtcct
     3541 tttgagcagt ttgctattca agtcaaacct gcagcagcct tacaagacga gcgtacctta
     3601 gaaaaactag aactagagcg tcgctattgg cagcaaaagc aaattccttg gttcattttt
     3661 actgataaag aaataaatcc cgtagtaaaa gaaaatattg aatggcttta ttcagtgaaa
     3721 acagaagaag tttctgcgga gcttttagca caactatccc cattggccca tatcctgcaa
     3781 gaaaaaggag atgaaaacat tatcaatgtc tgtaagcagg ttgatattgc ttatgatttg
     3841 gagttaggca aaacattgag tgagatacga gccttaaccg caaatggttt tattaagttc
     3901 aatatttata agtctttcag ggcaaataag tgtgcagatc tctgtattag ccaagtagtg
     3961 aatatggagg agttgcgcta tgtggcaaat taatgaggtt gtgctatttg ataatgatcc
     4021 gtatcgcatt ttggctatag aggatggcca agttgtctgg atgcaaataa gcgctgataa
     4081 aggagttcca caagctaggg ctgagttgtt gctaatgcag tatttagatg aaggccgctt
     4141 agttagaact gatgaccctt atgtacatct tgatttagaa gagccgtctg tagattctgt
     4201 cagcttccag aagcgcgagg aggattatcg aaaaattctt cctattatta atagtaagga
     4261 tcgtttcgac cctaaagtca gaagcgaact cgttgagcat gtggtccaag aacataaggt
     4321 tactaaggct acagtttata agttgttacg ccgttactgg cagcgtggtc aaacgcctaa
     4381 tgcattaatt cctgactaca aaaacagcgg tgcaccaggg gaaagacgtt cagcgacagg
     4441 aacagcaaag attggccgag ccagagaata tggtaagggt gaaggaacca aggtaacgcc
     4501 cgagattgaa cgccttttta ggttgaccat agaaaagcac ctgttaaatc aaaaaggtac
     4561 aaagaccacc gttgcctata gacgatttgt ggacttgttt gctcagtatt ttcctcgcat
     4621 tccccaagag gattacccaa cactacgtca gtttcgttat ttttatgatc gagaataccc
     4681 taaagctcag cgcttaaagt ctagagttaa agcaggggta tataaaaaag acgtacgacc
     4741 cttaagtagt acagccactt ctcaggcgtt aggccctggg agtcgttatg agattgatgc
     4801 cacgattgct gatatttatt tagtggatca tcatgatcgc caaaaaatca taggaagacc
     4861 aacgctttac attgtgattg atgtgtttag tcggatgatc acgggctttt atatcggctt
     4921 tgaaaatccg tcttatgtgg tggcgatgca ggcttttgta aatgcttgct ctgacaaaac
     4981 ggccatttgt gcccagcatg atattgagat tagtagctca gactggccgt gtgtaggttt
     5041 gccagatgtg ttgctagcgg accgtggcga attaatgagt catcaggtcg aagccttagt
     5101 ttctagtttt aatgtgcgag tggaaagtgc tccacctaga cgtggcgatg ctaaaggcat
     5161 agtggaaagc acttttagaa cactacaagc cgagtttaag tcctttgcac ctggcattgt
     5221 agagggcagt cggatcaaaa gccatggtga aacagactat aggttagatg catctctgtc
     5281 ggtatttgag ttcacacaaa ttattttgcg tacgatctta ttcagaaata accatctggt
     5341 gatggataaa tacgatcgag atgctgattt tcctacagat ttaccgtcta ttcctgtcca
     5401 gctatggcaa tggggtatgc agcatcgtac aggtagttta agggctgtgg agcaagagca
     5461 gttgcgagta gcgttactgc ctcgccgaaa ggtctctatt tcttcatttg gcgttaattt
     5521 gtggggtttg tattactcgg ggtcagagat tctgcgtgag ggttggttgc agcggagcac
     5581 tgatatagct agacctcaac atttagaagc ggcttatgac ccagtgctgg ttgatacgat
     5641 ttatttgttt ccgcaagttg gcagccgtgt attttggcgc tgtaatctga cggaacgtag
     5701 tcggcagttt aaaggtctct cattttggga ggtttgggat atacaagcac aagaaaaaca
     5761 caataaagcc aatgcgaagc aggatgagtt aactaaacgc agggagcttg aggcgtttat
     5821 tcagcaaacc attcagaaag cgaataagtt aacgcccagt actactgagc ccaaatcaac
     5881 acgcattaag cagattaaaa ctaataaaaa agaagccgtg acctcggagc gtaaaaaacg
     5941 tgcggagcat ttgaagccaa gctcttcagg tgatgaggct aaagttattc ctttcaacgc
     6001 agtggaagcg gatgatcaag aagattacag cctacccaca tacgtgcctg aattatttca
     6061 ggatccacca gaaaaggatg agtcatgagt gctacccgga ttcaagcagt ttatcgtgat
     6121 acgggggtag aggcttatcg tgataatcct tttatcgagg ccttaccacc attacaagag
     6181 tcagtgaata gtgctgcatc actgaaatcc tctttacagc ttacttcctc tgacttgcaa
     6241 aagtcccgtg ttatcagagc tcataccatt tgtcgtattc cagatgacta ttttcagcca
     6301 ttaggtacgc atttgctact aagtgagcgt atttcggtca tgattcgagg tggctacgta
     6361 ggcagaaatc ctaaaacagg agatttacaa aagcatttac aaaatggtta tgagcgtgtt
     6421 caaacgggag agttggagac atttcgcttt gaggaggcac gatctacggc acaaagctta
     6481 ttgttaattg gttgttctgg tagtgggaag acgacctctc ttcatcgtat tctagccacg
     6541 tatcctcagg tgatttacca tcgtgaactc aatgtagagc aggtggtgta tttgaaaata
     6601 gactgctcgc ataatggttc gctaaaagaa atctgcttga attttttcag agcgttggat
     6661 cgagccttgg gctcgaacta tgagcgtcgt tatggcttaa aacgtcatgg tatagaaacc
     6721 atgttggctt tgatgtcgca aatagccaat gcacatgctt tagggttgtt ggttattgat
     6781 gaaattcagc atttaagccg ctctcgttcg ggtggatctc aagagatgct gaactttttt
     6841 gtgacgatgg tgaatattat tggcgtacca gtgatgttga ttggtacccc taaagcacga
     6901 gagatttttg aggctgattt gcggtctgca cgtagagggg cagggtttgg agctatattc
     6961 tgggatccta tacaacaaac gcaacgtgga aagcccaatc aagagtggat cgcttttacg
     7021 gataatcttt ggcaattaca gcttttacaa cgcaaagatg cgctgttatc ggatgaggtc
     7081 cgtgatgtgt ggtatgagct aagccaagga gtgatggaca ttgtagtaaa actttttgta
     7141 ctcgctcagc tccgtgcgct agctttaggc aatgagcgta ttaccgctgg tttattgcgg
     7201 caagtgtatc aagatgagtt aaagcctgtg caccccatgc tagaggcatt acgctcgggt
     7261 atcccagaac gcattgctcg ttattctgat ctagtcgttc ccgagattga taaacggtta
     7321 atccaacttc agctagatat cgcagcgata caagaacaaa caccagaaga aaaagccctt
     7381 caagagttag ataccgaaga tcagcgtcat ttatatctga tgctgaaaga ggattacgat
     7441 tcaagcctgt taattcccac tattaaaaaa gcgtttagcc agaatccaac gatgacaaga
     7501 caaaagttac tgcctcttgt tttgcagtgg ttgatggaag gcgaaacggt agtgtcagaa
     7561 ctagaaaagc cctccaagag taaaaaggtt tcggctataa aggtagtcaa gcccagcgac
     7621 tgggatagct tgcctgatac ggatttacgt tatatctatt cacaacgcca acctgaaaaa
     7681 accatgcatg aacggttaaa agggaaaggg gtaatagtgg atatggcgag cttatttaaa
     7741 caagcaggtt agccatgaga aactttcctg ttccgtactc gaatgagctg atttatagca
     7801 ctattgcacg ggcaggcgtt tatcaaggga ttgttagtcc taagcagctg ttggatgagg
     7861 tgtatggcaa ccgcaaggtg gtcgctacct taggtctgcc ctcgcattta ggtgtgatag
     7921 caagacatct acatcaaaca ggacgttacg ctgttcagca gcttatttat gagcatacct
     7981 tattcccttt atatgctccg tttgtaggca aggagcgccg agacgaagct attcggttaa
     8041 tggagtacca agcgcaaggt gcggtgcatt taatgctagg agtcgctgct tctagagtta
     8101 agagcgataa ccgctttaga tactgccctg attgcgttgc tcttcagcta aataggtatg
     8161 gggaagcctt ttggcaacga gattggtatt tgcccgcttt gccatattgt ccaaaacacg
     8221 gtgctttagt cttctttgat agagctgtag atgatcaccg acatcaattt tgggctttgg
     8281 gtcatactga gctgctttca gactacccca aagactccct atctcaatta acagcactag
     8341 ctgcttatat agcccctctg ttagatgctc cacgagcgca agagctttcc ccaagccttg
     8401 agcagtggac gctgttttat cagcgcttag cgcaggatct agggctaacc aaaagcaagc
     8461 acattcgtca tgacttggtg gcggagagag tgaggcagac ttttagtgat gaggcactag
     8521 agaaactgga tttaaagttg gcagagaaca aggacacgtg ttggctgaaa agtatattcc
     8581 gtaagcatag aaaagccttt agttatttac agcatagtat tgtgtggcaa gccttattgc
     8641 caaaactaac ggttatagaa gcgctacagc aggcaagtgc tcttactgag cactctataa
     8701 cgacaagacc tgttagccag tctgtgcaac ctaactctga agatttatct gttaagcata
     8761 aagactggca gcaactagtg cataaatacc aaggaattaa ggcggcaaga cagtctttag
     8821 agggtggggt gctatacgct tggctttacc gacatgacag ggattggcta gttcactgga
     8881 atcaacagca tcaacaagag cgtctggcac ccgcccctag agttgattgg aaccaaagag
     8941 atcgaattgc tgtacgacaa ctattaagaa tcataaagcg tctagatagt agccttgatc
     9001 acccaagagc gacatcgagc tggctgttaa agcaaactcc taacggaacc tctcttgcaa
     9061 aaaatctaca gaaactgcct ttggtagcgc tttgcttaaa gcgttactca gagagtgtgg
     9121 aagattatca aattagacgg attagccaag cttttattaa gcttaaacag gaagatgttg
     9181 agcttaggcg ctggcgatta ttaagaagtg caacgttatc taaagagcgg ataactgagg
     9241 aagcacaaag attcttggaa atggtttatg gggaagagtg agtggttagg ctagctacat
     9301 ttaatgacaa tgtgcaggtt gtacatattg gtcatttatt ccgtaactcg ggtcataagg
     9361 agtggcgtat ttttgtttgg tttaatccaa tgcaagaacg gaaatggact cgatttactc
     9421 atttgccttt attaagtcga gctaaggtgg ttaacagtac aacaaagcaa ataaataagg
     9481 cggatcgtgt gattgagttt gaagcatcgg atcttcaacg agccaaaata atcgggatcc
     9541 tctagagtcg acctgcaggc atgcaagctt ggcactggcc gtcgttttac aacgtcgtga
     9601 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     9661 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     9721 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     9781 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     9841 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     9901 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     9961 acgcgcga
//
