LOCUS       sequence-232426-        9437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S291D into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, 
            Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA,
            Golshani A, Babu M
  TITLE     The conserved Tpk1 regulates non-homologous end joining 
            double-strand break repair by phosphorylation of Nej1, a homolog of 
            the human XLF.
  JOURNAL   Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 
            10.1093/nar/gkab585.
  PUBMED    34244791
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585."
COMMENT     Sequence Label: pLEX307_CR-PDHA2S291D
FEATURES             Location/Qualifiers
     source          1..9437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1500..1511
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2470..3058
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2523..2543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2941..2952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3130..3363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3441..3562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3478..3497)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3532..3551
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3602..3737
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3757..3776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3758..3776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3786..3803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3786..3802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3795..3817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3944..4399
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4031..4050)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4241..4262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4425..4529
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4530..4598,4599..5390)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4748..4767)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5561..6149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6050..6069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6303..6320
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6437..6458
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6473..6478,6479..6496,6497..6503)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6511..6527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6516..6538
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6535..6551
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6535..6551
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6570..6590
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6572..6590
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6618..6844
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6845..7025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7072..7197
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7690..7923
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8108..8152
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8383..8593
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8405..8601
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8452..8587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8633..9235
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8633..8652)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9129..9149
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9278..9395
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catgctggcc gccttcatct cccgcgtgtt gaggcgagtt
     1321 gcccagaaat cagctcgcag agtgctggtg gcatcccgta actcctcaaa tgacgctaca
     1381 tttgaaatta agaaatgtga tctttatctg ttggaagagg gtccccctgt cactacagtg
     1441 ctcactaggg cggaggggct taaatactac aggatgatgc tgactgttcg ccgcatggaa
     1501 ttgaaggcag atcagctgta caaacagaaa ttcattcgcg gtttctgtca cctgtgcgat
     1561 ggtcaggaag cttgttgcgt gggccttgag gccggcataa atccctcgga tcacgtcatt
     1621 acatcctata gggctcatgg tgtgtgctat actcggggac tttctgtccg atccattctc
     1681 gcagagctga cgggaagaag aggaggttgt gctaaaggaa aaggaggatc gatgcatatg
     1741 tataccaaga acttctatgg gggcaatggc atcgtcggtg cacagggccc cctgggcgct
     1801 ggcattgctc tggcctgtaa atataaagga aacgatgaga tctgtttgac tttatatggg
     1861 gatggcgctg cgaatcaggg gcagatagcc gaagctttca atatggcagc tttatggaaa
     1921 ttaccttgtg ttttcatctg tgagaataac ctatatggaa tgggaacatc tactgagaga
     1981 gcagcagcca gccctgatta ctacaagagg ggcaatttta tccctgggct aaaggtcgat
     2041 ggaatggatg ttctgtgtgt tcgtgaggca acaaaatttg cagctaacta ctgtagatct
     2101 ggaaaggggc ccatactgat ggagctgcaa acctaccgtt atcatggaca cgacatgagt
     2161 gatcctggag tcagttatcg tacacgagaa gaaattcagg aagtaagaag taagagggat
     2221 cctataataa ttctccaaga tagaatggta aacagcaagc tcgccactgt ggaagaatta
     2281 aaggaaattg gggctgaggt gaggaaagaa attgatgatg ctgcccagtt tgctaccact
     2341 gatcctgagc cacatttgga agaattaggc catcacatct acagcagtga ttcatctttt
     2401 gaagttcgtg gtgcaaatcc atggatcaag tttaagtccg tcagttaaac tagtacgcgt
     2461 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     2521 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     2581 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     2641 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     2701 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     2761 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     2821 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     2881 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     2941 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3001 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     3061 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     3121 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     3181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     3241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     3301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     3361 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     3421 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     3481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3541 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     3601 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     3661 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     3721 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     3781 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     3841 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     3901 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     3961 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4021 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4081 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4141 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4201 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4261 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     4321 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4381 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     4441 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4501 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     4561 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4621 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4681 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4741 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4801 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4861 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4921 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4981 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     5041 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     5101 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     5161 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     5221 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     5281 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     5341 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     5401 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5461 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5701 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6241 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     6301 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     6361 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     6421 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     6481 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     6541 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     6601 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     6661 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     6721 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     6781 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     6841 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6901 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6961 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7021 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     7081 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     7141 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     7201 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     7261 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     7321 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     7381 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     7441 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     7501 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     7561 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     7621 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     7681 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     7741 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     7801 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     7861 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     7921 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     7981 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     8041 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     8101 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     8161 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     8221 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     8281 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     8341 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8401 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     8461 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8521 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8581 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     8641 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     8701 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     8761 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     8821 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     8881 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8941 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     9001 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     9061 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     9121 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     9181 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     9241 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     9301 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     9361 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     9421 atccactttg gcgccgg
//
