LOCUS       sequence-232429-        9437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S291A into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pLEX307_CR-PDHA2S291A
FEATURES             Location/Qualifiers
     source          1..9437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1405..1416
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2375..2963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2428..2448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2846..2857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3035..3268
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3346..3467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3383..3402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3437..3456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3507..3642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3662..3681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3663..3681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3691..3708)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3691..3707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3700..3722)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3849..4304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3936..3955)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4146..4167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4330..4434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4435..4503,4504..5295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4653..4672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5466..6054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5955..5974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6208..6225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6342..6363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6378..6383,6384..6401,6402..6408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6416..6432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6421..6443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6440..6456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6440..6456
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6475..6495
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6477..6495
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6523..6749
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6750..6930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6977..7102
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7595..7828
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8013..8057
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8288..8498
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8310..8506
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8357..8492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8538..9140
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8538..8557)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9034..9054
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9183..9300
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatgc tggccgcctt
     1201 catctcccgc gtgttgaggc gagttgccca gaaatcagct cgcagagtgc tggtggcatc
     1261 ccgtaactcc tcaaatgacg ctacatttga aattaagaaa tgtgatcttt atctgttgga
     1321 agagggtccc cctgtcacta cagtgctcac tagggcggag gggcttaaat actacaggat
     1381 gatgctgact gttcgccgca tggaattgaa ggcagatcag ctgtacaaac agaaattcat
     1441 tcgcggtttc tgtcacctgt gcgatggtca ggaagcttgt tgcgtgggcc ttgaggccgg
     1501 cataaatccc tcggatcacg tcattacatc ctatagggct catggtgtgt gctatactcg
     1561 gggactttct gtccgatcca ttctcgcaga gctgacggga agaagaggag gttgtgctaa
     1621 aggaaaagga ggatcgatgc atatgtatac caagaacttc tatgggggca atggcatcgt
     1681 cggtgcacag ggccccctgg gcgctggcat tgctctggcc tgtaaatata aaggaaacga
     1741 tgagatctgt ttgactttat atggggatgg cgctgcgaat caggggcaga tagccgaagc
     1801 tttcaatatg gcagctttat ggaaattacc ttgtgttttc atctgtgaga ataacctata
     1861 tggaatggga acatctactg agagagcagc agccagccct gattactaca agaggggcaa
     1921 ttttatccct gggctaaagg tcgatggaat ggatgttctg tgtgttcgtg aggcaacaaa
     1981 atttgcagct aactactgta gatctggaaa ggggcccata ctgatggagc tgcaaaccta
     2041 ccgttatcat ggacacgcca tgagtgatcc tggagtcagt tatcgtacac gagaagaaat
     2101 tcaggaagta agaagtaaga gggatcctat aataattctc caagatagaa tggtaaacag
     2161 caagctcgcc actgtggaag aattaaagga aattggggct gaggtgagga aagaaattga
     2221 tgatgctgcc cagtttgcta ccactgatcc tgagccacat ttggaagaat taggccatca
     2281 catctacagc agtgattcat cttttgaagt tcgtggtgca aatccatgga tcaagtttaa
     2341 gtccgtcagt taaactagta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     2401 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2461 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2521 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2581 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2641 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2701 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2761 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2821 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2881 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2941 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     3001 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3061 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     3121 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     3181 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     3241 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     3301 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     3361 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3421 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     3481 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3541 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3601 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     3661 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     3721 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3781 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3841 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3901 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3961 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4021 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4081 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4141 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4201 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4261 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     4321 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4381 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4441 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4501 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4561 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4621 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4681 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4741 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4801 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4861 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4921 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4981 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5041 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5101 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5161 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5221 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5281 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5341 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5401 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5461 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5521 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5581 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5641 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5701 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5761 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5821 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5881 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5941 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6001 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6061 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6121 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6181 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6241 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6301 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6361 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6421 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     6481 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     6541 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     6601 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     6661 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     6721 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6901 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6961 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7021 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7081 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     7141 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     7201 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     7261 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     7321 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     7381 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7441 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     7501 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7561 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7621 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7681 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7741 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7801 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7861 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7921 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7981 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     8041 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     8101 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     8161 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     8221 cgaggggacc catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8281 ctccccagca ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc
     8341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg
     8521 taccaccgga gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     8581 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     8641 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     8701 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg
     8761 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     8821 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     8881 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     8941 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     9001 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     9061 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     9121 gcaagcccgg tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac
     9181 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     9241 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     9301 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc
     9361 aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct
     9421 tgaaaggagt gggaatt
//
