LOCUS       sequence-232438-       10056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  enables eukaryotic expression of human EDB (or EIIIB) containing 
            fibronectin based on plasma fibronectin sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Kraft S, Klemis V, Sens C, Lenhard T, Jacobi C, Samstag Y, Wabnitz 
            G, Kirschfink M, Wallich R, Hansch GM, Nakchbandi IA
  TITLE     Identification and characterization of a unique role for EDB 
            fibronectin in phagocytosis.
  JOURNAL   J Mol Med (Berl). 2016 May;94(5):567-81. doi: 
            10.1007/s00109-015-1373-0. Epub 2015 Dec 5.
  PUBMED    26637426
REFERENCE   2  (bases 1 to 10056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00109-015-1373-0"; journalName: "J Mol Med (Berl)"; date: 
            "2016-05"; volume: "94"; issue: "5"; pages: "567-81"
COMMENT     Sequence Label: Fibronectin-human-EDB in pMAX
FEATURES             Location/Qualifiers
     source          1..10056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          813..945
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(8229..8248)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    8258..8339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8283..8302
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8537..9125)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8617..8636)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9206..10000)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(9206..10000)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9317..9336)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9927..9946
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
      721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
      841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
      901 cacctattgg tcttactgac atccactttg cctttctctc cacaggggta ccgccatcat
      961 gaagtttaaa caagcttgaa ttctctagag atatcctgca gagatctgga tccctcgagg
     1021 ctagctgtca acatgcttag gggtccgggg cccgggctgc tgctgctggc cgtccagtgc
     1081 ctggggacag cggtgccctc cacgggagcc tcgaagagca agaggcaggc tcagcaaatg
     1141 gttcagcccc agtccccggt ggctgtcagt caaagcaagc ccggttgtta tgacaatgga
     1201 aaacactatc agataaatca acagtgggag cggacctacc taggcaatgc gttggtttgt
     1261 acttgttatg gaggaagccg aggttttaac tgcgagagta aacctgaagc tgaagagact
     1321 tgctttgaca agtacactgg gaacacttac cgagtgggtg acacttatga gcgtcctaaa
     1381 gactccatga tctgggactg tacctgcatc ggggctgggc gagggagaat aagctgtacc
     1441 atcgcaaacc gctgccatga agggggtcag tcctacaaga ttggtgacac ctggaggaga
     1501 ccacatgaga ctggtggtta catgttagag tgtgtgtgtc ttggtaatgg aaaaggagaa
     1561 tggacctgca agcccatagc tgagaagtgt tttgatcatg ctgctgggac ttcctatgtg
     1621 gtcggagaaa cgtgggagaa gccctaccaa ggctggatga tggtagattg tactcgcctg
     1681 ggagaaggca gcggacgcat cacttgcact tctagaaata gatgcaacga tcaggacaca
     1741 aggacatcct atagaattgg agacacctgg agcaagaagg ataatcgagg aaacctgctc
     1801 cagtgcatct gcacaggcaa cggccgagga gagtggaagt gtgagaggca cacctctgtg
     1861 cagaccacat cgagcggatc tggccccttc accgatgttc gtgcagctgt ttaccaaccg
     1921 cagcctcacc cccagcctcc tccctatggc cactgtgtca cagacagtgg tgtggtctac
     1981 tctgtgggga tgcagtggct gaagacacaa ggaaataagc aaatgctttg cacgtgcctg
     2041 ggcaacggag tcagctgcca agagacagct gtaacccaga cttacggtgg caactcaaat
     2101 ggagagccat gtgtcttacc attcacctac aatggcagga cgttctactc ctgcaccaca
     2161 gaagggcgac aggacggaca tctttggtgc agtacaactt cgaattatga gcaggaccag
     2221 aaatactctt tctgcacaga ccacactgtt ttggttcaga ctcgaggagg aaattccaat
     2281 ggtgccttgt gccacttccc cttcctatac aacaaccaca attacactga ttgcacttct
     2341 gagggcagaa gagacaacat gaagtggtgt gggaccacac agaactatga tgccgaccag
     2401 aagtttgggt tctgccccat ggctgcccac gaggaaatct gcacaaccaa tgaaggggtc
     2461 atgtaccgca ttggagatca gtgggataag cagcatgaca tgggtcacat gatgaggtgc
     2521 acgtgtgttg ggaatggtcg tggggaatgg acatgcattg cctactcgca gcttcgagat
     2581 cagtgcattg ttgatgacat cacttacaat gtgaacgaca cattccacaa gcgtcatgaa
     2641 gaggggcaca tgctgaactg tacatgcttc ggtcagggtc ggggcaggtg gaagtgtgat
     2701 cccgtcgacc aatgccagga ttcagagact gggacgtttt atcaaattgg agattcatgg
     2761 gagaagtatg tgcatggtgt cagataccag tgctactgct atggccgtgg cattggggag
     2821 tggcattgcc aacctttaca gacctatcca agctcaagtg gtcctgtcga agtatttatc
     2881 actgagactc cgagtcagcc caactcccac cccatccagt ggaatgcacc acagccatct
     2941 cacatttcca agtacattct caggtggaga cctaaaaatt ctgtaggccg ttggaaggaa
     3001 gctaccatac caggccactt aaactcctac accatcaaag gcctgaagcc tggtgtggta
     3061 tacgagggcc agctcatcag catccagcag tacggccacc aagaagtgac tcgctttgac
     3121 ttcaccacca ccagcaccag cacacctgtg accagcaaca ccgtgacagg agagacgact
     3181 cccttttctc ctcttgtggc cacttctgaa tctgtgaccg aaatcacagc cagtagcttt
     3241 gtggtctcct gggtctcagc ttccgacacc gtgtcgggat tccgggtgga atatgagctg
     3301 agtgaggagg gagatgagcc acagtacctg gatcttccaa gcacagccac ttctgtgaac
     3361 atccctgacc tgcttcctgg ccgaaaatac attgtaaatg tctatcagat atctgaggat
     3421 ggggagcaga gtttgatcct gtctacttca caaacaacag cgcctgatgc ccctcctgac
     3481 ccgactgtgg accaagttga tgacacctca attgttgttc gctggagcag accccaggct
     3541 cccatcacag ggtacagaat agtctattcg ccatcagtag aaggtagcag cacagaactc
     3601 aaccttcctg aaactgcaaa ctccgtcacc ctcagtgact tgcaacctgg tgttcagtat
     3661 aacatcacta tctatgctgt ggaagaaaat caagaaagta cacctgttgt cattcaacaa
     3721 gaaaccactg gcaccccacg ctcagataca gtgccctctc ccagggacct gcagtttgtg
     3781 gaagtgacag acgtgaaggt caccatcatg tggacaccgc ctgagagtgc agtgaccggc
     3841 taccgtgtgg atgtgatccc cgtcaacctg cctggcgagc acgggcagag gctgcccatc
     3901 agcaggaaca cctttgcaga agtcaccggg ctgtcccctg gggtcaccta ttacttcaaa
     3961 gtctttgcag tgagccatgg gagggagagc aagcctctga ctgctcaaca gacaaccaaa
     4021 ctggatgctc ccactaacct ccagtttgtc aatgaaactg attctactgt cctggtgaga
     4081 tggactccac ctcgggccca gataacagga taccgactga ccgtgggcct tacccgaaga
     4141 ggccagccca ggcagtacaa tgtgggtccc tctgtctcca agtaccccct gaggaatctg
     4201 cagcctgcat ctgagtacac cgtatccctc gtggccataa agggcaacca agagagcccc
     4261 aaagccactg gagtctttac cacactgcag cctgggagct ctattccacc ttacaacacc
     4321 gaggtgactg agaccaccat tgtgatcaca tggacgcctg ctccaagaat tggttttaag
     4381 ctgggtgtac gaccaagcca gggaggagag gcaccacgag aagtgacttc agactcagga
     4441 agcatcgttg tgtccggctt gactccagga gtagaatacg tctacaccat ccaagtcctg
     4501 agagatggac aggaaagaga tgcgccaatt gtaaacaaag tggtgacacc attgtctcca
     4561 ccaacaaact tgcatctgga ggcaaaccct gacactggag tgctcacagt ctcctgggag
     4621 aggagcacca ccccagacat tactggttat agaattacca caacccctac aaacggccag
     4681 cagggaaatt ctttggaaga agtggtccaa gctgatcaga gctcctgcac ttttgataac
     4741 ctgagtcccg gcctggagta caatgtcagt gtttacactg tcaaggatga caaggaaagt
     4801 gtccctatct ctgataccat catcccagag gtgccccaac tcactgacct aagctttgtt
     4861 gatataaccg attcaagcat cggcctgagg tggaccccgc taaactcttc caccattatt
     4921 gggtaccgca tcacagtagt tgcggcagga gaaggtatcc ctatttttga agattttgtg
     4981 gactcctcag taggatacta cacagtcaca gggctggagc cgggcattga ctatgatatc
     5041 agcgttatca ctctcattaa tggcggcgag agtgccccta ctacactgac acaacaaacg
     5101 gctgttcctc ctcccactga cctgcgattc accaacattg gtccagacac catgcgtgtc
     5161 acctgggctc cacccccatc cattgattta accaacttcc tggtgcgtta ctcacctgtg
     5221 aaaaatgagg aagatgttgc agagttgtca atttctcctt cagacaatgc agtggtctta
     5281 acaaatctcc tgcctggtac agaatatgta gtgagtgtct ccagtgtcta cgaacaacat
     5341 gagagcacac ctcttagagg aagacagaaa acaggtcttg attccccaac tggcattgac
     5401 ttttctgata ttactgccaa ctcttttact gtgcactgga ttgctcctcg agccaccatc
     5461 actggctaca ggatccgcca tcatcccgag cacttcagtg ggagacctcg agaagatcgg
     5521 gtgccccact ctcggaattc catcaccctc accaacctca ctccaggcac agagtatgtg
     5581 gtcagcatcg ttgctcttaa tggcagagag gaaagtccct tattgattgg ccaacaatca
     5641 acagtttctg atgttccgag ggacctggaa gttgttgctg cgacccccac cagcctactg
     5701 atcagctggg atgctcctgc tgtcacagtg agatattaca ggatcactta cggagaaaca
     5761 ggaggaaata gccctgtcca ggagttcact gtgcctggga gcaagtctac agctaccatc
     5821 agcggcctta aacctggagt tgattatacc atcactgtgt atgctgtcac tggccgtgga
     5881 gacagccccg caagcagcaa gccaatttcc attaattacc gaacagaaat tgacaaacca
     5941 tcccagatgc aagtgaccga tgttcagggc aacagcatta gtgtcaagtg gctgccttca
     6001 agttcccctg ttactggtta cagagtaacc accactccca aaaatggacc aggaccaaca
     6061 aaaactaaaa ctgcaggtcc agatcaaaca gaaatgacta ttgaaggctt gcagcccaca
     6121 gtggagtatg tggttagtgt ctatgctcag aatccaagcg gagagagtca gcctctggtt
     6181 cagactgcag taaccactat tcctgcacca actgacctga agttcactca ggtcacaccc
     6241 acaagcctga gcgcccagtg gacaccaccc aatgttcagc tcactggata tcgagtgcgg
     6301 gtgaccccca aggagaagac cggaccaatg aaagaaatca accttgctcc tgacagctca
     6361 tccgtggttg tatcaggact tatggtggcc accaaatatg aagtgagtgt ctatgctctt
     6421 aaggacactt tgacaagcag accagctcag ggtgttgtca ccactctgga gaatgtcagc
     6481 ccaccaagaa gggctcgtgt gacagatgct actgagacca ccatcaccat tagctggaga
     6541 accaagactg agacgatcac tggcttccaa gttgatgccg ttccagccaa tggccagact
     6601 ccaatccaga gaaccatcaa gccagatgtc agaagctaca ccatcacagg tttacaacca
     6661 ggcactgact acaagatcta cctgtacacc ttgaatgaca atgctcggag ctcccctgtg
     6721 gtcatcgacg cctccactgc cattgatgca ccatccaacc tgcgtttcct ggccaccaca
     6781 cccaattcct tgctggtatc atggcagccg ccacgtgcca ggattaccgg ctacatcatc
     6841 aagtatgaga agcctgggtc tcctcccaga gaagtggtcc ctcggccccg ccctggtgtc
     6901 acagaggcta ctattactgg cctggaaccg ggaaccgaat atacaattta tgtcattgcc
     6961 ctgaagaata atcagaagag cgagcccctg attggaagga aaaagacaga cgagcttccc
     7021 caactggtaa cccttccaca ccccaatctt catggaccag agatcttgga tgttccttcc
     7081 acagttcaaa agaccccttt cgtcacccac cctgggtatg acactggaaa tggtattcag
     7141 cttcctggca cttctggtca gcaacccagt gttgggcaac aaatgatctt tgaggaacat
     7201 ggttttaggc ggaccacacc gcccacaacg gccaccccca taaggcatag gccaagacca
     7261 tacccgccga atgtaggtga ggaaatccaa attggtcaca tccccaggga agatgtagac
     7321 tatcacctgt acccacacgg tccgggactc aatccaaatg cctctacagg acaagaagct
     7381 ctctctcaga caaccatctc atgggcccca ttccaggaca cttctgagta catcatttca
     7441 tgtcatcctg ttggcactga tgaagaaccc ttacagttca gggttcctgg aacttctacc
     7501 agtgccactc tgacaggcct caccagaggt gccacctaca acatcatagt ggaggcactg
     7561 aaagaccagc agaggcataa ggttcgggaa gaggttgtta ccgtgggcaa ctctgtcaac
     7621 gaaggcttga accaacctac ggatgactcg tgctttgacc cctacacagt ttcccattat
     7681 gccgttggag atgagtggga acgaatgtct gaatcaggct ttaaactgtt gtgccagtgc
     7741 ttaggctttg gaagtggtca tttcagatgt gattcatcta gatggtgcca tgacaatggt
     7801 gtgaactaca agattggaga gaagtgggac cgtcagggag aaaatggcca gatgatgagc
     7861 tgcacatgtc ttgggaacgg aaaaggagaa ttcaagtgtg accctcatga ggcaacgtgt
     7921 tatgatgatg ggaagacata ccacgtagga gaacagtggc agaaggaata tctcggtgcc
     7981 atttgctcct gcacatgctt tggaggccag cggggctggc gctgtgacaa ctgccgcaga
     8041 cctgggggtg aacccactcc cgaaggcact actggccagt cctacaacca gtattctcag
     8101 agataccatc agagaacaaa cactaatgtt aattgcccaa ttgagtgctt catgccttta
     8161 gatgtacagg ctgacagaga agattcccga gagtaaatca tctttgcggc cgcgtttaaa
     8221 cagagctcga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     8281 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     8341 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     8401 aaagcaagta aaacctctac aaatgtggta cttaagaggg ggagaccaaa gggcgagacg
     8461 ttaaggcctc acgtgacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8521 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8581 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8641 gaagctccct cgtgcgctct cctgttccga ccctgccgct tacgggatac ctgtccgcct
     8701 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8761 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8821 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8881 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8941 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9001 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9061 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9181 gttaagggat tttggtcatg ccgtctcaga agaactcgtc aagaaggcga tagaaggcga
     9241 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     9301 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     9361 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     9421 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga
     9481 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     9541 cgacaagacc ggcttccatc cgagtacgtg ctctctcgat gcgatgtttc gcttggtggt
     9601 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     9661 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     9721 atagcagcca gtcccttccc gcttcagtga caacgtcgag tacagctgcg caaggaacgc
     9781 ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg
     9841 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     9901 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     9961 cggccggaga acctgcgtgc aatccatctt gttcaatcat aatattattg aagcatttat
    10021 cagggttcgt ctcatcccgg tctcctccca tgcatg
//
