LOCUS       sequence-232446-        6188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for heterologous protein secretion in Lactobacillus 
            plantarum and L. sakei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Mathiesen G, Sveen A, Brurberg MB, Fredriksen L, Axelsson L, Eijsink
            VG
  TITLE     Genome-wide analysis of signal peptide functionality in 
            Lactobacillus plantarum WCFS1.
  JOURNAL   BMC Genomics. 2009 Sep 10;10:425. doi: 10.1186/1471-2164-10-425.
  PUBMED    19744343
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2009-09-10"; volume: "10:425. doi"; pages: " 
            10.1186/1471-2164-10-425"
COMMENT     Sequence Label: pLp_3050sNuc
FEATURES             Location/Qualifiers
     source          1..6188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      917..1504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1405..1424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1658..1675
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atatgaaaaa atttaacttt aaaaccatgt tgctattagt tttggctagt tgtgtcttcg
       61 gggtcgtcgt taacgtgact actagtcttg gaccacaaac cgcaatcacc gcccaggcct
      121 ccaaggtcga cccattgtca actaaaaaat tacataaaga acctgcgact ttaattaaag
      181 cgattgatgg tgatacggtt aaattaatgt acaaaggtca accaatgaca ttcagactat
      241 tattggttga tacacctgaa acaaagcatc ctaaaaaagg tgtagagaaa tatggtcctg
      301 aagcaagtgc atttacgaaa aaaatggtag aaaatgcaaa gaaaattgaa gtcgagtttg
      361 acaaaggtca aagaactgat aaatatggac gtggcttagc gtatatttat gctgatggaa
      421 aaatggtaaa cgaagcttta gttcgtcaag gcttggctaa agttgcttat gtttacaaac
      481 ctaacaatac acatgaacaa catttaagaa aaagtgaagc acaagcgaaa aaagagaaat
      541 taaatatttg gagcgaagac aacgctgatt caggtcaata agaattcggt accccgggtt
      601 cgaaggcgcc aagcttcaaa ttacagcacg tgttgctttg attgatagcc aaaaagcagc
      661 agttgataaa gcaattactg atattgctga aaaattgtaa tttataaata aaaatcacct
      721 tttagaggtg gtttttttat ttataaatta ttcgtttgat ttcgctttcg atagaacaat
      781 caaagcgaga ataaggaaga taaatcccat aagggcggga gcagaatgtc cgagactaat
      841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1381 gaggcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatc
     1501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1561 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1621 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgagaagga
     1681 ttattcggct ggttgagacg ttaaaatgat aaaggttgta ttaatcttat attacggtta
     1741 taatgtactc aacttaataa atgaacgcaa aaaaaagaac cctcaactta gcagagttag
     1801 gattcacgac ttatcagcac aacctgataa gattttcgat agcaagtact accaatacaa
     1861 gctatctaac ttggtactat tataacatgt aggctaagtt tttcaaccat tgatacttaa
     1921 agtaaacggt tgttatcggg aatcttaaca gaaacctgat agcaaccgtt tttttgttat
     1981 tcaatggtta gcaaccatca aagcaactaa aggctggaaa cctgttctta gctagtaaaa
     2041 cctcccgtga gtgtcgttcg tgaccccgct tgcagttaac aacataggta tgctaaacct
     2101 tgtcgagatc aacgcgacta aagacgtggc tggaagacta ggaaatgata cggacaggct
     2161 aactattaac gcagattatt cgggttgctg ctaaaaccaa ctctaataat agttagtgca
     2221 agggctggtt gagcttaaat tgtctgataa agagttctct ctttatactg caaaagaagc
     2281 gcagttattc acgattagga taactgtttg agagagccta agggcttgac ccttgatggt
     2341 ttaagcaccg ctatgcgtgc gggatcccct tagaagcaaa cttaagagtg tgttgatagt
     2401 gcattatctt aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatagga
     2461 attgaagtta aattagatgc taaaaatttg taattaagaa ggagggattc gtcatgttgg
     2521 tattccaaat gcgtaatgta gataaaacat ctactgtttt gaaacagact aaaaacagtg
     2581 attacgcaga taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt
     2641 ttaaacagat aactaaaatt acaaacaaat cgtttaactt caggagagat tacatgaaca
     2701 aaaatataaa tatctcaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac
     2761 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
     2821 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
     2881 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
     2941 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttaca
     3001 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc
     3061 tgactgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
     3121 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
     3181 ctaaaccaaa agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
     3241 ataaatattg gaagctatat aagtactttg tttcaaaatg ggtcaatcga gaatatcgtc
     3301 aactgtttac taaaaatcag tttcgtcaag caatgaaaca cgccaaagta aacaatttaa
     3361 gtaccattac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
     3421 ggaaataatt ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat
     3481 ggagaccggg gcccttcaat agagttctta acgttaatcc gaaaaaaact aacgttaata
     3541 ttaaaaaata agatccgctt gtgaattatg tataatttga ttagactaaa gaataggaga
     3601 aagtatgatg atatttaaaa aactttctcg ttaagatagg ttgttggtga gcatgttata
     3661 tacggatgta tcggtttcct taatgcaaaa ttttgttgct atcttattaa tttttctatt
     3721 atatagatat attcaaagaa agataacatt taaacggatc atattagata ttttaatagc
     3781 gattattttt tcaatattat atctgtttat ttcagatgcg tcattacttg taatggtatt
     3841 aatgcgatta gggtggcatt ttcatcaaca aaaagaaaat aagataaaaa cgactgatac
     3901 agctaattta attctaatta tcgtgatcca gttattgtta gttgcggttg ggactattat
     3961 tagtcagttt accatatcga ttatcaaaag tgatttcagc caaaatatat tgaacaatag
     4021 tgcaacagat ataactttat taggtatttt ctttgctgtt ttatttgacg gcttgttctt
     4081 tatattattg aagaataagc ggactgaatt acaacattta aatcaagaaa tcattgaatt
     4141 ttcgttagaa aaacaatatt ttatatttat atttatttta tttatagtaa tagaaattat
     4201 tttagcagtt gggaatcttc aaggagtaac agccacgata ttattaacca ttatcattat
     4261 tttttgtgtc cttatcggga tgactttttg gcaagtgatg ctttttttga aggcttattc
     4321 gattcgccaa gaagccaatg accaattggt ccggaatcaa caacttcaag attatctagt
     4381 caatatcgaa cagcagtaca ccgaattacg gcgatttaag catgattatc aaaacatctt
     4441 attatcgttg gagagttttg ccgaaaaggg cgatcagcaa cagtttaagg cgtattacca
     4501 agaattatta gcacaacggc caattcaaag tgaaatccaa ggggcagtca ttgcacaact
     4561 cgactacttg aaaaatgatc ctattcgagg attagtcatt caaaagtttt tggcagccaa
     4621 acaggctggt gttactttaa aattcgaaat gaccgaacca atcgaattag caaccgctaa
     4681 tctattaacg gttattcgga ttatcggtat tttattagac aatgcgattg aacaagccgt
     4741 tcaagaaacc gatcaattgg tgagttgtgc tttcttacaa tctgatggtt taatcgaaat
     4801 tacgattgaa aatacggcca gtcaagttaa gaatctccaa gcattttcag agttaggcta
     4861 ttcaacgaaa ggcgctggtc gggggactgg tttagctaat gtgcaggatt tgattgccaa
     4921 acaaaccaat ttattcttag aaacacagat tgaaaataga aagttacgac agacattgat
     4981 gattacggag gaaacttaat ttgtatcccg tttatttatt agaggatgat ttacagcaac
     5041 aagcgattta tcagcaaatt atcgcgaata cgattatgat taacgaattt gcaatgactt
     5101 taacatgcgc tgccagtgat actgagacat tgttggcggc aattaaggat cagcaacgag
     5161 gtttattctt tttggatatg gaaattgagg ataaccgcca agccggttta gaagtggcaa
     5221 ctaagattcg gcagatgatg ccgtttgcgc aaattgtctt cattacaacc cacgaggaac
     5281 tgacattatt aacgttagaa cgaaaaatag cgcctttaga ttacattctc aaggaccaaa
     5341 caatggctga aatcaaaagg caattgattg atgatctatt gttagctgag aagcaaaacg
     5401 aggcggcagc gtatcaccga gaaaatttat ttagttataa aataggtcct cgctttttct
     5461 cattaccatt aaaggaagtt gtttatttat atactgaaaa agaaaatccg ggtcatatta
     5521 atttgttagc cgttaccaga aaggttactt ttccaggaaa tttaaatgcg ctggaagccc
     5581 aatatccaat gctctttcgg tgtgataaaa gttacttagt taacctatct aatattgcca
     5641 attatgacag taaaacacgg agtttaaaat ttgtagatgg cagtgaggca aaagtctcgt
     5701 tccggaaatc acgggaacta gtggccaaat taaaacaaat gatgtagcgc ctgcagcacg
     5761 ccaaatgatc ccagtaaaaa gccacccgca tggcgggtgg ctttttatta gccctagaag
     5821 ggcttcccac acgcatttca gcgccttagt gccttagttt gtgaatcata ggtggtatag
     5881 tcccgaaata cccgtctaag gaattgtcag ataggcctaa tgactggctt ttataatatg
     5941 agataatgcc gactgtactt tttacagtcg gttttctaat gtcactaacc tgccccgtta
     6001 gttgaagaag gtttttatat tacagctcca gatctaccgg tgggcccata ttaacgttta
     6061 accgataaag ttgaacgtta atattttttt tgcgcagaaa tggtaaattg aagcataata
     6121 gtcttgtaag gtatttagct ggctggcgta aagtatgctt tataaaataa tatataggag
     6181 tatgattc
//
