LOCUS       sequence-232449-       10556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Lerner AM, Yumerefendi H, Goudy OJ, Strahl BD, Kuhlman B
  TITLE     Engineering Improved Photoswitches for the Control of 
            Nucleocytoplasmic Distribution.
  JOURNAL   ACS Synth Biol. 2018 Nov 29. doi: 10.1021/acssynbio.8b00368.
  PUBMED    30441907
REFERENCE   2  (bases 1 to 10556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Nov 29. doi: 10.1021/acssynbio.8b00368."
COMMENT     Sequence Label: pNIA-CEN-FLAG-LINXa4(RLR)-Bre1
FEATURES             Location/Qualifiers
     source          1..10556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             759..782
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             834..1262
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTERLRDAAEREGVMLIKKTAENIDEAAKEL"
     terminator      3426..3673
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3442..3460)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3691..3710)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3692..3710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3720..3737)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3720..3736)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3729..3751)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3872..3889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4043..4631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4123..4142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4802..5593,5594..5662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5425..5444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5663..5767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5835..5853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5873..6278
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6279..7373
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    7735..8897
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      7735..8572
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     protein_bind    9635..9656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9671..9676,9677..9694,9695..9701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9709..9725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9714..9736
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9733..9749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9733..9749
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9768..9788
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9770..9788
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactccaag cttgaattaa
      721 ttccgggcgg aatgaaagcg ttaacgtctg gcggccgcga ttacaaggac gacgatgaca
      781 agtctagagc cgccgccaag agggtgaagc tggacggttc tggaagtggg tccttggcta
      841 ctacacttga acgtattgag aagaactttg tcattactga cccaagattg ccagataatc
      901 ccattatatt cgcgtccgat agtttcttgc agttgacaga atatagccgt gaagaaattt
      961 tgggaagaaa ctgcaggttt ctacaaggtc ctgaaactga tcgcgcgaca gtgagaaaaa
     1021 ttagagatgc catagataac caaacagagg tcactgttca gctgattaat tatacaaaga
     1081 gtggtaaaaa gttctggaac ctctttcact tgcagcctat gcgagatcag aagggagatg
     1141 tccagtactt tattggggtt cagttggatg gaactgagcg actgcgagat gctgcagaga
     1201 gagagggagt catgctgatt aagaaaactg cagaaaatat tgatgaagcg gcaaaagagc
     1261 tggccgggct ggatctgggg tctggaagtg ggtcggggtc ccggggaacg gccgagcctg
     1321 ctactgccgc tatcgcacta gaattaagcg atcctagtga accgcttact caatctgatg
     1381 tgatagcgtt tcaaaaagaa gctttgttta gatgtataaa tcgtcgaaga gtcgactttg
     1441 aagccttgcg aaagcaatat gaactttcaa gaagggagtg catagatgtt tcccgcaagt
     1501 tggcaaatat tatggcgctg atagtaactt tagctaggtt tatcgaaaca ttctgcacag
     1561 acgccaacga gaagcaacta tgtagggaaa tagcccaagg cgatgagacg ttgatagttc
     1621 agcgcagtga ttctttcatg aagctactga ctaaatatgg gaagccaaac acgacagata
     1681 gtaatactaa tagtaatgct tcggatcata ttcaagaatt aacaacggag ttaaaaaact
     1741 tgcggaaaag taaagaggag ctcttttatg aaaattcgca attgacagag gagatttctg
     1801 ctttgaagga atattataca aatataattc gcaagtatga tagagatgaa tcctttacta
     1861 taaaacgtgt ttttaaggaa gataagactg atgctgtaaa ggaattacgg gaagatgaaa
     1921 aagaatctaa tgaaaacaat ataaaatctg gaaataaaga ctcctctgca attaatggtg
     1981 ataatacatc caaaaaatcc gagaaaggtg atgaacttgt gcaggcggag gatgaacgaa
     2041 aagaagatgc tgagaatgaa aaattagaat tggatctgaa attttctgat ttacgggcag
     2101 agataaactc cttgagttcc actataaaag accttgaaaa tatcagaagg ggaaacgagg
     2161 aagagctcat caaaacacgt tctgaggtat ccaatttaaa aaagcaacag atagcagcag
     2221 cagaccaaga cccagatttc aagtcatatg accatgagtc cctgttagca aaaattcaac
     2281 atttgactga acaaaacgca gaattatctg agatcaatag ttccttttta tcaaaatttc
     2341 aagttttagc caaagagaaa gaaatatata cgaataaggt ccgagaagaa ttccagaaaa
     2401 gtctcgactc cctagtagaa atgaacagtt cattggaaaa ggacgttgta agaataagaa
     2461 cggctcgtga tgatttacta agcaaaatcg ccattttgga agctgaaaaa tccaagactg
     2521 aagtgttgag cgatttacaa catgcaattg atattttgaa gaagcaatgg acaaaaattg
     2581 atcaaagatc aaacgataca aaatcctcgt cgactcaaga tgcattaata aaggaaattc
     2641 aagacttaga gaagggtttt agagagttat ccgacttaac tcataaaaag tactctgaga
     2701 ttattaatca cgaatctgtt atatccaagc ttaccgtaga aaaaactaag gctgatcaga
     2761 aatattttgc cgcgatgagg tccaaagatt caattttaat tgaaatcaaa actctttcta
     2821 aaagcctcag taaatcaaat gagcttattc tacagttaaa agactctgat agattgctac
     2881 agcaaaaaat aggaaattta cataaacaac tggatctttc tcaaaataat gaacgcaggt
     2941 taattgattc cagtaaaaca gagacactaa aaataattga tttaaataat acatccacta
     3001 aactgaaaag gtcattagaa aagctacaag aagaaagtaa caaaagcatt gcagatatga
     3061 cgcatctaga aactaaatta aatgatacag aaatagaatt gaagcacttc aagcagaagg
     3121 catctcatct agaatccaag tgtgaaaaac tacatgatac attgttccgt ggtaacaaca
     3181 aaaataaggg ctcgagcgat gaagcactcg tagaagaact agcaaatttc cgtacacttg
     3241 tttattgttc cctgtgttct aaaaattgga aaaacatggc tattaaaacc tgtggccatg
     3301 tcttttgtga gaattgctgt aaggaacgat tggcagcgag aatgagaaaa tgccctacat
     3361 gcaacaaagc attcagttcc aatgatttat tgacagtgca cttgtaagga tccgtcgacc
     3421 tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca catccgctct
     3481 aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt tttttatagt
     3541 tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc tgtacagacg
     3601 cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg gacgctcgaa
     3661 ggctttaatt tgcggccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     3721 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3781 cttgcagcac atcccccttt cgccagctgc attaatgaat cggccaacgc gcggggagag
     3841 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     3901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     3961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4441 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4681 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     4921 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     4981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5581 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5641 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5701 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5761 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     5821 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttaactgtgg
     5881 gaatactcag gtatcgtaag atgcaagagt tcgaatctct tagcaaccat tatttttttc
     5941 ctcaacataa cgagaacaca caggggcgct atcgcacaga atcaaattcg atgactggaa
     6001 attttttgtt aatttcagag gtcgcctgac gcatatacct ttttcaactg aaaaattggg
     6061 agaaaaagga aaggtgagag cgccggaacc ggcttttcat atagaataga gaagcgttca
     6121 tgactaaatg cttgcatcac aatacttgaa gttgacaata ttatttaagg acctattgtt
     6181 ttttccaata ggtggttagc aatcgtctta ctttctaact tttcttacct tttacatttc
     6241 agcaatatat atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg
     6301 tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta
     6361 aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg
     6421 gtgctgctat cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga
     6481 aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtaca ggtagtgtta
     6541 gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac
     6601 catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta
     6661 aaggtactga cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa
     6721 aggaagacga tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc
     6781 aaagaatcac aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt
     6841 ggtccttgga taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg
     6901 aaaccatcaa gaacgaattt cctacattga aggttcaaca tcaattgatt gattctgccg
     6961 ccatgatcct agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt
     7021 ttggtgatat catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat
     7081 ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc
     7141 acggttctgc tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg
     7201 ctgcaatgat gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg
     7261 cagttaaaaa ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta
     7321 ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt
     7381 ctcttttttt atgatatttg tacataaact ttataaatga aattcataat agaaacgaca
     7441 cgaaattaca aaatggaata tgttcatagg gtagaattaa ttctcatgtt tgacagctta
     7501 tcatcgatcg tccaactgca tggagatgag tcgtggcaag aataccaaga gttcctcggt
     7561 ttgccagtta ttaaaagact cgtatttcca aaagactgca acatactact cagtgcagct
     7621 tcacagaaac ctcattcgtt tattcccttg tttgattcag aagcaggtgg gacaggtgaa
     7681 cttttggatt ggaactcgat ttctgactgg gttggaaggc aagagagccc cgagagctta
     7741 cattttatgt tagctggtgg actgacgcca gaaaatgttg gtgatgcgct tagattaaat
     7801 ggcgttattg gtgttgatgt aagcggaggt gtggagacaa atggtgtaaa agactctaac
     7861 aaaatagcaa atttcgtcaa aaatgctaag aaataggtta ttactgagta gtatttattt
     7921 aagtattgtt tgtgcacttg cctgcaagcc ttttgaaaag caagcataaa agatctaaac
     7981 ataaaatctg taaaataaca agatgtaaag ataatgctaa atcatttggc tttttgattg
     8041 attgtacagg aaaatataca tcgcaggggg ttgactttta ccatttcacc gcaatggaat
     8101 caaacttgtt gaagagaatg ttcacaggcg catacgctac aatgacccga ttcttgctag
     8161 ccttttctcg gtcttgcaaa caaccgccgg cagcttagta tataaataca catgtacata
     8221 cctctctccg tatcctcgta atcattttct tgtatttatc gtcttttcgc tgtaaaaact
     8281 ttatcacact tatctcaaat acacttatta accgctttta ctattatctt ctacgctgac
     8341 agtaatatca aacagtgaca catattaaac acagtggttt ctttgcataa acaccatcag
     8401 cctcaagtcg tcaagtaaag atttcgtgtt catgcagata gataacaatc tatatgttga
     8461 taattagcgt tgcctcatca atgcgagatc cgtttaaccg gaccctagtg cacttacccc
     8521 acgttcggtc cactgtgtgc cgaacatgct ccttcactat tttaacatgt ggaattaatt
     8581 ctcatgtttg acagcttatc atcgaactct aagaggtgat acttatttac tgtaaaactg
     8641 tgacgataaa accggaagga agaataagaa aactcgaact gatctataat gcctattttc
     8701 tgtaaagagt ttaagctatg aaagcctcgg cattttggcc gctcctaggt agtgcttttt
     8761 ttccaaggac aaaacagttt ctttttcttg agcaggtttt atgtttcggt aatcataaac
     8821 aataaataaa ttatttcatt tatgtttaaa aataaaaaat aaaaaagtat tttaaatttt
     8881 taaaaaagtt gattataagc atgtgacctt ttgcaagcaa ttaaattttg caatttgtga
     8941 tttaggcaaa agttactatt tctggctcgt gtaatatatg tatgctaatg tgaactttta
     9001 caaagtcgat atggacttag tcaaaagaaa ttttcttaaa aatatatagc actagccaat
     9061 ttagcacttc tttatgagat atattataga ctttattaag ccagatttgt gtattatatg
     9121 tatttacccg gcgaatcatg gacatacatt ctgaaatagg taatattctc tatggtgaga
     9181 cagcatagat aacctaggat acaagttaaa agctagtact gttttgcagt aatttttttc
     9241 ttttttataa gaatgttacc acctaaataa gttataaagt caatagttaa gtttgatatt
     9301 tgattgtaaa ataccgtaat atatttgcat gatcaaaagg ctcaatgttg actagccagc
     9361 atgtcaacca ctatattgat caccgatatt aggacttcca caccaactag taatatgaca
     9421 ataaattcaa gatattcttc atgagaatgg cccagcgatt atgcggtgtg aaataccgca
     9481 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
     9541 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcac gacaggtttc
     9601 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9661 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     9721 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     9781 actaaaggga acaaaagctg gagctcgccg ggatcgaaga aatgatggta aatgaaatag
     9841 gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa ataaagtgaa
     9901 aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta cgcaattgca
     9961 caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata acgctgggcg
    10021 tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac cctgcgctaa
    10081 ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg acgaccacga
    10141 caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa catgagtata
    10201 ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga acaatagagc
    10261 gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag aggaaacagc
    10321 aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat ggttgtctgt
    10381 ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata tggaagggaa
    10441 ctttacactt ctcctatgca catatattaa ttaaagtcca atgctagtag agaagggggg
    10501 taacacccct ccgcgctctt ttccgatttt tttctaaacc gtggaatatt tcggat
//
