LOCUS       sequence-232460-        5342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  precusor outer membrane protein A, ompA, truncated after amino acid 
            176.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Bariya P, Randall LL
  TITLE     Co-assembly of SecYEG and SecA fully restores the properties of the 
            native translocon.
  JOURNAL   J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18.
  PUBMED    30275279
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18."
COMMENT     Sequence Label: pAL832
FEATURES             Location/Qualifiers
     source          1..5342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(100..105,106..122,123..129)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    137..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     142..164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     sig_peptide     330..392
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     primer_bind     complement(1433..1450)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1603..1689
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1781..1808
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1827..1918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1919..1987,1988..2779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2137..2156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2950..3538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3439..3458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3692..3709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3724..3864
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3790..3809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3950..3972
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        4050..4127
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4128..5210
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4147..4166)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
       61 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatccggct
      121 cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag accatggaat
      181 tcccagactt tacatcgcca ggggtgctcg gcataagccg aagatatcgg tagagttaat
      241 attgagcaga tcccccggtg aaggatttaa ccgtgttatc tcgttggaga tattcatggc
      301 gtattttgga tgataacgag gcgcaaaaaa tgaaaaagac agctatcgcg attgcagtgg
      361 cactggctgg tttcgctacc gtagcgcagg ccgctccgaa agataacacc tggtacactg
      421 gtgctaaact gggctggtcc cagtaccatg acactggttt catcaacaac aatggcccga
      481 cccatgaaaa ccaactgggc gctggtgctt ttggtggtta ccaggttaac ccgtatgttg
      541 gctttgaaat gggttacgac tggttaggtc gtatgccgta caaaggcagc gttgaaaacg
      601 gtgcatacaa agctcagggc gttcaactga ccgctaaact gggttaccca atcactgacg
      661 acctggacat ctactgtcgt ctgggtggca tggtatggcg tgcagacact aaatccaacg
      721 tttatggtaa aaaccacgac accggcgttt ctccggtctt cgctggcggt gttgagtacg
      781 cgatcactcc tgaaatcgct acccgtctgg aataccagtg gaccaacaac atcggtgacg
      841 cacacacctg cggcactcgt ccggacaacg gcatgctgag cctgggtgtt tcctaccgtt
      901 tcggtcaggg cgaggcagct taataagttg ctccggctcc agctccggca ccggaagtac
      961 agaccaagca cttcactctg aagtctgacg ttctgttcaa cttcaacaaa gcaaccctga
     1021 aaccggaagg tcaggctgct ctggatcagc tgtacagcca gctgagcaac ctggatccga
     1081 aagacggttc cgtagttgtt ctgggttaca ccgaccgcat cggttctgac gcttacaacc
     1141 agggtctgtc cgagcgccgt gctcagtctg ttgttgatta cctgatctcc aaaggtatcc
     1201 cggcagacaa gatctccgca cgtggtatgg gcgaatccaa cccggttact ggcaacacct
     1261 ctgacaacgt gaaacagcgt gctgcactga tcgactgcct ggctccggat cgtcgcgtag
     1321 agatcgaagt taaaggtatc aaagacgttg taactcagcc gcaggcttaa tctagagtcg
     1381 acctgcaggc atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac
     1441 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     1501 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     1561 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     1621 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1681 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     1741 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     1801 ggcctttttg cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta
     1861 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1921 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1981 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2041 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2101 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2161 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2221 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2281 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2341 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2401 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2461 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2521 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2581 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2641 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2701 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2761 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2821 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3061 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3601 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3661 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3721 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     3781 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     3841 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     3901 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3961 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagcagat caattcgcgc
     4021 gcgaaggcga agcggcatgc atttacgttg acaccatcga atggtgcaaa acctttcgcg
     4081 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
     4141 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
     4201 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
     4261 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
     4321 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
     4381 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
     4441 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
     4501 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
     4561 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
     4621 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
     4681 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
     4741 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
     4801 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
     4861 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
     4921 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
     4981 cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     5041 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     5101 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5161 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     5221 attaatgtaa gttagcgcga attgatctgg tttgacagct tatcatcgac tgcacggtgc
     5281 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
     5341 at
//
