LOCUS       sequence-23252-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Pusl T, Wu JJ, Zimmerman TL, Zhang L, Ehrlich BE, Berchtold MW, Hoek
            JB, Karpen SJ, Nathanson MH, Bennett AM
  TITLE     Epidermal growth factor-mediated activation of the ETS domain 
            transcription factor Elk-1 requires nuclear calcium.
  JOURNAL   J Biol Chem. 2002 Jul 26. 277(30):27517-27.
  PUBMED    11971908
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2002 Jul 26. 277(30):27517-27."
COMMENT     Sequence Label: pCMV-PV-NLS-CDEF-GFP
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             399..419
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             423..443
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             447..467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             480..509
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(545..562)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    551..662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cacgtggaaa ttaatacgac gtggccacca tggcccaggt gcagctgcag gtcgacatgt
       61 cgatgacaga cttgctcagc gctgaggaca tcaagaaggc gataggagcc tttactgctg
      121 cagactcctt cgaccacaaa aagttcttcc agatggtggg cctgaagaaa aagagtgcgg
      181 atgatgtgaa gaaggtgttc cacattctgg ccaaagacaa aagtggcttc attgaggagg
      241 atgtgctggg gtccattctg aagggcttct cctcagatgc cagagacttg tctgctaagg
      301 aaacaaagac gctgatggct gctggagcca aggacgggga cggcaagatt ggggttgaag
      361 tgtggtccac tctggtggcc gaaagcgcgg ccgcagatcc aaaaaagaag agaaaggtag
      421 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat acggccgcag
      481 aacaaaaact catctcagaa gaggatctga atggggccgc atagtctaga agctcgctga
      541 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      601 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      661 tcgcattgtc tgagtaggtg tcat
//
