LOCUS       sequence-23253-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Sano T, Pandori MW, Chen X, Smith CL, Cantor CR
  TITLE     Recombinant core streptavidins. A minimum-sized core streptavidin 
            has enhanced structural stability and higher accessibility to 
            biotinylated macromolecules.
  JOURNAL   J Biol Chem. 1995 Nov 24. 270(47):28204-9.
  PUBMED    7499314
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1995 Nov 24. 270(47):28204-9."
COMMENT     Sequence Label: pTSA-13
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             431..463
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(517..535)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      531..578
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttttgtttac tttaagaagg agatatacat agtgggcatc accggcacct ggtacaacca
       61 gctcggctcg accttcatcg tgaccgcggg cgccgacggc gccctgaccg gaacctacga
      121 gtcggccgtc ggcaacgccg agagccgcta cgtcctgacc ggtcgttacg acagcgcccc
      181 ggccaccgac ggcagcggca ccgccctcgg ttggacggtg gcctggaaga ataactaccg
      241 caacgcccac tccgcgacca cgtggagcgg ccagtacgtc ggcggcgccg aggcgaggat
      301 caacacccag tggctgctga cctccggcac caccgaggcc aacgcctgga agtccacgct
      361 ggtcggccac gacaccttca ccaaggtgta gccgtccgcc gcctccatcg acgcggcgaa
      421 gaaggcccat atggctagca tgactggtgg acagcaaatg ggtcgcggat ccggctgcta
      481 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      541 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      601 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
      661 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctc
//
